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EC number: 252-743-7 | CAS number: 35835-94-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 March 2018 - 04 April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): A mixed population of sewage treatment micro-organisms was obtained on 5 March 2018 from the final effluent stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.
The sample of effluent was filtered through coarse filter paper (first approximate 200 mL discarded) and maintained on aeration in a temperature controlled room at temperatures of between 20 and 21 ºC prior to use. - Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: as recommended in the OECD Guidelines
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: between 21 and 23 ºC
- pH: 7.4
- pH adjusted: no
- Continuous darkness: diffuse light
TEST SYSTEM
- Culturing apparatus: 500 mL in 500 mL bottles
- Number of culture flasks/concentration: 3 (test item, inoculum blank), 2 (reference item, toxicity control)
- Measuring equipment: CES Multi-Channel Aerobic Respirometer
- Other: All vessels were inoculated with the prepared inoculum at a rate of 1% v/v.
The system consists of a sample flask sealed by a sensor head/CO2 trap immersed in a temperature controlled water bath. The samples were stirred for the duration of the test with a magnetically coupled stirrer.
As biodegradation progresses, the micro-organisms convert oxygen to carbon dioxide which is absorbed into the ethanolamine solution (50% v/v).
SAMPLING
- Sampling frequency: daily Biological Oxygen Demand (BOD) values
CONTROL AND BLANK SYSTEM
- Inoculum blank: Three replicate bottles containing inoculated mineral medium
- Reference item (procedural control): Two replicate bottles containing inoculated mineral medium and the reference item, aniline, at a concentration of 100 mg/L.
- Toxicity control: Two replicate bottles containing in inoculated mineral medium plus the reference item, aniline, at a concentration of 100 mg/L and the test item at a concentration of 100 mg/L - Reference substance:
- aniline
- Parameter:
- % degradation (O2 consumption)
- Value:
- 12
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- toxicity control
- Value:
- 53
- Sampling time:
- 14 d
- Details on results:
- The test item attained 12% biodegradation after 28 days, calculated from the oxygen consumption values, and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
The toxicity control attained 53% biodegradation after 14 days and 56% biodegradation after 28 days thereby confirming that the test item was not toxic to the sewage treatment micro-organisms used in the test. - Results with reference substance:
- Aniline (procedure control) attained 76% biodegradation after 14 days and 79% biodegradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item attained 12% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
- Executive summary:
This study was performed to assess the ready biodegradability of ETPPAAc in an aerobic aqueous media. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301F, “Ready Biodegradability; Manometric Respirometry Test” referenced as method C.4-D of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (q)).
The test item at a concentration of 100 mg/L was exposed to sewage treatment micro-organisms with mineral medium in sealed culture vessels in diffuse light at temperatures of between 21 and 23 ºC for 28 days.
The biodegradation of the test item was assessed by the measurement of daily oxygen consumption values on Days 0 to 28. Control solutions with inoculum and the reference item, aniline, together with a toxicity control were used for validation purposes.
The toxicity control attained 53% biodegradation after 14 days and 56% biodegradation after 28 days thereby confirming that the test item was not toxic to the sewage treatment micro-organisms used in the test.
Aniline (procedure control) attained 76% biodegradation after 14 days and 79% biodegradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
The test item attained 12% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
Reference
Percentage Biodegradation Values
Day |
% Biodegradation |
||||
Procedure Control |
Test Item |
Toxicity Control |
|||
Replicate 1 |
Replicate 2 |
Mean |
|||
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
2 |
1 |
8 |
7 |
8 |
3 |
3 |
1 |
10 |
10 |
10 |
4 |
4 |
16 |
11 |
11 |
11 |
6 |
5 |
38 |
12 |
12 |
12 |
20 |
6 |
55 |
12 |
12 |
12 |
37 |
7 |
62 |
12 |
12 |
12 |
46 |
8 |
69 |
13 |
12 |
13 |
49 |
9 |
72 |
13 |
12 |
13 |
50 |
10 |
74 |
13 |
12 |
13 |
50 |
11 |
75 |
13 |
12 |
13 |
51 |
12 |
75 |
13 |
12 |
13 |
52 |
13 |
76 |
13 |
12 |
13 |
52 |
14 |
76 |
13 |
12 |
13 |
53 |
15 |
76 |
13 |
12 |
13 |
53 |
16 |
77 |
13 |
12 |
13 |
53 |
17 |
77 |
13 |
12 |
13 |
54 |
18 |
77 |
13 |
12 |
13 |
54 |
19 |
77 |
13 |
12 |
13 |
54 |
20 |
78 |
13 |
12 |
13 |
55 |
21 |
78 |
13 |
12 |
13 |
55 |
22 |
78 |
13 |
12 |
13 |
55 |
23 |
78 |
12 |
11 |
12 |
55 |
24 |
78 |
12 |
11 |
12 |
55 |
25 |
78 |
12 |
11 |
12 |
56 |
26 |
78 |
12 |
11 |
12 |
56 |
27 |
79 |
12 |
11 |
12 |
56 |
28 |
79 |
12 |
11 |
12 |
56 |
Validation Criteria
The mean BOD of the inoculated mineral medium (control) was 28.24 mg O2/L after 28 days and therefore satisfied the validation criterion given in the OECD Test Guidelines.
The pH of the inoculated test item vessels on Day 28 were 7.9 and hence satisfied the validation criterion given in the OECD Test Guidelines.
The difference between extremes of replicate BOD values at the end of the test was less than 20% and therefore satisfied the validation criterion given in the OECD Test Guidelines.
Description of key information
12% degradation after 28 d (OECD TG 301F)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
This study was performed to assess the ready biodegradability of ETPPAAc in an aerobic aqueous media. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No. 301F, “Ready Biodegradability; Manometric Respirometry Test” referenced as method C.4-D of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (q)).
The test item at a concentration of 100 mg/L was exposed to sewage treatment micro-organisms with mineral medium in sealed culture vessels in diffuse light at temperatures of between 21 and 23 ºC for 28 days.
The biodegradation of the test item was assessed by the measurement of daily oxygen consumption values on Days 0 to 28. Control solutions with inoculum and the reference item, aniline, together with a toxicity control were used for validation purposes.
The toxicity control attained 53% biodegradation after 14 days and 56% biodegradation after 28 days thereby confirming that the test item was not toxic to the sewage treatment micro-organisms used in the test.
Aniline (procedure control) attained 76% biodegradation after 14 days and 79% biodegradation after 28 days thereby confirming the suitability of the inoculum and test conditions.
The test item attained 12% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301F.
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