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Diss Factsheets
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EC number: 424-650-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: GB/T 21809-2008: Chemicals-Test Method of Earthworm Acute Toxicity Test
- Principles of method if other than guideline:
- - Principle of test: acute toxicity study to earthworms over 14 day period
- Short description of test conditions:
Artificial soil treated with the test substance
Four replicate plus control
Parameters were observed at start of test, 7th day and end of test (day 14)
- Parameters analysed / observed:
Mortality
Abnormal behaviour - GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): high-speed blender for 5 minutes
- Controls: artificial soil - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm
- Source: Shanghai Yonghe Biotech Earthworm Breeding Farm
- Age at test initiation: 3 months
- Weight at test initiation (mean and SD): 0.46 +/- 0.08g
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): healthy, no mortality observed - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 20°C +/- 2°C
- pH:
- 6.12 - 6.26
- Moisture:
- 35% (Humidity = 80%)
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): Glass 1L, perforated lid
- Amount of soil or substrate: 750g (wet weight)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 2
- No. of replicates per control: 2
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): Sphagnum Peat 10% (pH 5.5, no visible plant remains), Kaolin Clay 20% (Kaolinite content was 45%), Industrial Sand 70%
- Maximum water holding capacity (in % dry weight): 35%
OTHER TEST CONDITIONS
- Photoperiod: Continuous light
- Light intensity: 600-800 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
2 minutes after start of test, 7 days, 14 days
VEHICLE CONTROL PERFORMED: yes
- Filter paper contact test with chloroacetamide (A.R.) to deterrmine sensitivity of earthworms
- 1 earthworm/concentration
- test duration: 24 hours in dark, 20 +/-2°C
- Observation taken with 30 secs of initiation of test then 24h.
TEST CONCENTRATIONS
- Limit test: 1000 mg/kg
- Range finding study (7 days)
- Test concentrations: 0, 100, 1000 mg/kg
- Results used to determine the conditions for the definitive study: yes - Nominal and measured concentrations:
- Nominal: 1000 mg/kg
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LD50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: No
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 24h LC50 3.63 x 10-3 mg/cm2
- within historical range - 1.5 x 10-3 to 4.5 x 10-3 mg/cm2 - Validity criteria fulfilled:
- yes
- Conclusions:
- The 14d LC50 for the test material was was determined to be >1000 mg/kg. No mortality was observed in the test.
Reference
Description of key information
In acute toxicity to earthworm study conducted in accordance with GLP, the 14 day LD50 to earthworms was deterrmined to be greater than the highest test concentration (1000mg/kg)
Key value for chemical safety assessment
Additional information
It is not appropriate to include an EC50 value for the chemical safety assessment above as this is determined to be greater than the highest concentration tested
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