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EC number: 268-776-5 | CAS number: 68140-14-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- It is considered appropriate to address the data requirements for DTO_DETA by read-across to the available studies on the main components of DTO_DETA: AAI_DETA and Rosin.
DTO_DETA and AAI_DETA are each a mixture of constituents which include monoamide, diamide, residual amine and imidazoline (mono-, di- and tri-condensate) chemical structures. The substances therefore have common functional groups based on amide, amine and imidazoline moieties and are sufficiently similar in terms of chemical structure to support a read-across approach.
DTO_DETA contains comparatively lower levels of imidazolines and higher levels of resin acids than AAI_DETA and therefore consideration of data for resin acids is also considered necessary. The main resin acid in DTO_DETA is abietic acid, but abietic acid derivatives and other acids, such as pimaric acid, are also found in notable quantities, and the resin acids collectively are known as ‘rosin’. DTO_DETA contains upto 25% unreacted rosin, and taking into account the compositional information available for the rosin in DTO_DETA and Rosin (CAS# 8050-09-07, EC# 232-475-7), the latter was considered appropriate for read-across to DTO_DETA.
The AAI_DETA dataset is worst case with regards to biodegradability and aquatic toxicity, with the dataset for Rosin showing a lower toxicity profile. Therefore, AAI_DETA is considered to be a worst case read-across source for the DTO_DETA target substance in the context of toxicity to soil macroorganisms. - Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 56 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 56 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 944 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality at end of exposure period: After 28 days of exposure to the test item no noteworthy mortality of adult earthworms (≤ 5%) occurred in the control as well as at all test item concentrations
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: At test start the earthworms had an individual weight of 0.30 - 0.60 g. The body weights of the earthworms increased in the control and at all test item concentrations throughout the first 28 days. The biomass increase was not statistically significant compared to the control at all test item concentrations
- No. of offspring produced: The mean number of juveniles in the control was 323. The average number of juveniles in the test item concentrations ranged between 265 and 347. Compared to the control the reproduction of the earthworms was not statistically significant at 125, 250, 500 and 1000 mg/kg soil dry weight. At 62.5 mg/kg soil dry weight the earthworm reproduction rate was statistically significantly reduced compared to the control.
- Behavioural abnormalities: No symptoms or changes in the behaviour of the earthworms were observed in the control as well as at all test item concentrations after 28 days of exposure to the test item - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 1.0 mg Carbendazim/kg soil DW - Reported statistics and error estimates:
- One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to the control. A Normality Test and an Equal Variance Test were conducted prior to running the ANOVA. P-values for both Normality and Equal Variance Test are 0.05. The a-value for ANOVA test (acceptable probability of incorrectly concluding that there is a difference) is a = 0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of the target substance DTO_DETA to soil macroorganisms is estimated to be a NOEC = 944.0 mg a.i./kg soil dw, based on read-across from a study testing AAI_DETA.
- Executive summary:
The toxicity of the target substance DTO_DETA to soil macroorganisms is estimated to be a NOEC = 944.0 mg a.i./kg soil dw, based on read-across from a study testing AAI_DETA.
As explained in the justification for type of information, DTO_DETA and AAI_DETA are each a mixture of constituents which include monoamide, diamide, residual amine and imidazoline (mono-, di- and tri-condensate) chemical structures. The substances therefore have common functional groups based on amide, amine and imidazoline moieties and are sufficiently similar in terms of chemical structure to support a read-across approach.
DTO_DETA contains comparatively lower levels of imidazolines and higher levels of resin acids than AAI_DETA and therefore consideration of data for resin acids is also considered necessary. The main resin acid in DTO_DETA is abietic acid, but abietic acid derivatives and other acids, such as pimaric acid, are also found in notable quantities, and the resin acids collectively are known as ‘rosin’. DTO_DETA contains upto 25% unreacted rosin, and taking into account the compositional information available for the rosin in DTO_DETA and Rosin (CAS# 8050-09-07, EC# 232-475-7), the latter was considered appropriate for read-across to DTO_DETA.
The AAI_DETA dataset is worst case with regards to biodegradability and aquatic toxicity, with the dataset for Rosin showing a lower toxicity profile. No data for the toxicity to soil macroorganisms are available for Rosin. Therefore, AAI_DETA is considered to be a worst case read-across source for the DTO_DETA target substance in the context of toxicity to soil macroorganisms.
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-01-22 to 2010-03-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, GLP, All validity criteria fulfilled, complete identification of test substance
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
- Version / remarks:
- DIN ISO 11268-2: 1998 (E), BBA Guideline (1994)
- Deviations:
- yes
- Remarks:
- Room temperature increased once for about four hours to 23 °C. . The soil moisture of all test vessels deviated by more than 10 % of the initial moisture at the end of the test. No pre-moistening of the natural soil.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Tall oil diethylenetriamine imidazoline (CAS No. 68442-97-7)
- Physical state: Liquid, amber, viscous
- Analytical purity: 94.4 %
- Purity test date: 2009-05-11
- Lot/batch No.: S-000922
- Expiration date of the lot/batch: 2017-07-02
- Water solubility: Insoluble
- Density: 980 kg/m3 at 20 °C
- pH: 10 - 12 at 75 % solution
- Storage condition of test material: Room temperature, protected from moisture and light, under nitrogen - Analytical monitoring:
- no
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): The test item was weighed out and was evenly distributed on 10 g quartz sand (vehicle) per replicate. The treated quartz sand was evenly distributed over the natural soil. Demineralised water was added to adjust the humidity of the natural soil to a moisture content of 56 % of the maximum water holding capacity.
Thereafter, the test medium was mixed for at least three minutes and about 600 g soil dry weight were filled into each test vessel.
- Controls: Natural soil with the addition of quartz sand (10 g pre replicate) without test or reference item.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): quartz sand
- Concentration of vehicle in test medium (stock solution and final test solution): 10 g per replicate
- Evaporation of vehicle before use: Not necessary - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Eisenia fetida SAVIGNY 1826, subspecies Eisenia fetida fetida
- Source: Own breeding
- Age at test initiation (mean and range, SD): synchronized earthworm culture, not deviating more than 1 month
- Weight at test initiation (mean and range, SD): 0.51 g
ACCLIMATION
- Acclimation period: 2 days prior to test start
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): healthy - Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 56 d
- Remarks:
- Removement of adults after 28 days
- Test temperature:
- 20 +/- 2°C
- pH:
- Application rate
[mg/kg soil dry weight] pH-value
day 0 day 56
Control 5.47 6.22
62.5 5.64 6.39
125 5.61 6.31
250 5.65 6.29
500 5.74 6.33
1000 5.95 6.28 - Moisture:
- Application rate
[mg/kg soil dry weight] Moisture [%] of dry weight
day 0 day 60
Control 25.5 29.3*
62.5 26.5 29.7*
125 26.6 29.5*
250 26.2 30.5*
500 25.5 28.4*
1000 24.9 29.1* - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): round plastic boxes, ID: 15 cm, bottom surface area: 177 cm3, height: 14 cm, transparent and perforated lids enabled sufficient gas exchange and light penetration and prevented the test substrate from drying
- Amount of soil or substrate: 600 g DW
- No. of organisms per container (treatment): 10 (40)
- No. of replicates per treatment group: 4
- No. of replicates per control: 8
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
A loamy sand (LUFA 2.2) soil (field fresh sampled) was used in the study. The soil was received by the LANDWIRTSCHAFTLICHE UNTERSUCHUNGS- UND FORSCHUNGSANSTALT SPAYER, Obere Langgasse 40, 67346 Speyer, Germany. The soil was sampled at January 4th, 2010 and stored at 6 ± 2 °C in the test facility until use. The soil parameters, determined before the beginning of the definitive study, are listed in Table 3. The soil history (years 2005 to 2009) is given below:
Pesticides:
Sampling year and 4 former years: None
Plants:
2009: Meadow
2008: Meadow
2007: Meadow
2006: Meadow
2005: Meadow
Fertilization:
No organic fertilization
2009: None
2008: None
2007: 600 kg/ha CaO
+ 250 kg/ha MgO
2006: None
2005: None
- Composition (natural soil):
Parameter LUFA-soil 2.2
Batch-No. F 2.20210
pH-value* 5.5 ± 0.1
Soil moisture before application [%] 14.1
Maximum water capacity* [g/100 g soil dry weight] 46.5 ± 6.0
Particle size distribution acc. to DIN*
Sand:
0.63 – 2.0 mm (%) 0.7 ± 0.2
0.2 – 0.63 mm (%) 44.5 ± 2.6
0.063 – 0.2 mm (%) 35.5 ± 2.2
Silt:
0.02 – 0.063mm (%) 5.8 ± 1.1
0.006 – 0.02 mm (%) 4.0 ± 0.6
0.002 – 0.006mm (%) 3.0 ± 0.6
Clay:
< 0.002 mm (%) 6.4 ± 0.9
- Organic carbon (%): 2.09 +/- 0.4
- Maximum water holding capacity (in % dry weigth): 46.5
- CEC: 10.0 +/- 0.5 mVal/100 g
- Pretreatment of soil: none
- Storage (condition, duration): 14 days after receipt at test conditions in the dark
OTHER TEST CONDITIONS
- Photoperiod: 16 h light - 8 h dark
- Light intensity: 560 +/- 88 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Adult mortality, biomass, behaviour and pathological symptoms after 28 days
Reproduction (Number of juveniles) after 56 days
VEHICLE CONTROL PERFORMED: No
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Nominal and measured concentrations:
- Nominal application rates: 62.5 - 125 - 250 - 500 - 1000 mg test substance/kg soil dry weight (DW)
- Reference substance (positive control):
- yes
- Remarks:
- 1.0 - 2.5 - 5.0 mg Carbendazim/ kg soil DW
- Duration:
- 56 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 56 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 944 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Details on results:
- - Mortality at end of exposure period: After 28 days of exposure to the test item no noteworthy mortality of adult earthworms (≤ 5%) occurred in the control as well as at all test item concentrations
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: At test start the earthworms had an individual weight of 0.30 - 0.60 g. The body weights of the earthworms increased in the control and at all test item concentrations throughout the first 28 days. The biomass increase did not differ statistically significant compared to the control at all test item concentrations
- No. of offspring produced: The mean number of juveniles in the control was 323. The average number of juveniles in the test item concentrations ranged between 265 and 347 (Table 8). Compared to the control the reproduction of the earthworms did not statistically significant differ at 125, 250, 500 and 1000 mg/kg soil dry weight. At 62.5 mg/kg soil dry weight the earthworm reproduction rate was statistically significantly reduced compared to the control.
- Behavioural abnormalities: No symptoms or changes in the behaviour of the earthworms were observed in the control as well as at all test item concentrations after 28 days of exposure to the test item - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 1.0 mg Carbendazim/kg soil DW - Reported statistics and error estimates:
- One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to
the control. A Normality Test and an Equal Variance Test were conducted prior to running the ANOVA. P-values for both Normality and Equal Variance Test are 0.05. The a-value for ANOVA test (acceptable probability of incorrectly concluding that there is a difference) is a = 0.05. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study, Tall oil diethylenetriamine imidazoline did not induce evident mortality or pathological symptoms of adult earthworms after an exposure to the test item concentrations of 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight for 28 days. The earthworm biomass increased in the control and at all tested application rates over 28 days without any statistically significant differences. After further four weeks the earthworm reproduction rate was not statistically significantly reduced compared to the control at 125, 250, 500 and 1000 mg/kg soil dry weight, whilst at the lowest concentration of 62.5 mg/kg soil dry weight a slight significant reduction was observed. Since this effect on earthworm reproduction only occurs at the lowest test item concentration, this effect is not considered to be a test item related effect (no dose response).
Thus, the No Observed Effect Concentration (NOEC) of Tall oil diethylenetriamine imidazoline with respect to mortality, body weight and reproduction of Eisenia fetida after 8 weeks of exposure in natural soil was determined to be 1000 mg/kg soil dry weight. - Executive summary:
Effects of Tall oil diethylenetriamine imidazoline (batch number S000922) on mortality, biomass and the reproductive potential of the earthworm species Eisenia fetida (Savigny) were determined according to the guidelines DIN ISO 11268-2 (1998), OECD 222 (2004) and BBA (1994) from January 22nd to March 23rd, 2010 with the definitive exposure phase from January 22nd to March 19th, 2010 at Dr.U.Noack-Laboratorien in 31157 Sarstedt, Germany. The study was conducted under static conditions over 8 weeks with five different application rates of 62.5 - 125 - 250 - 500 - 1000 mg/kg soil dry weight, which were mixed into natural soil. Eighty sexually mature earthworms were inserted into 8 control replicates (10 earthworms per replicate) and forty sexually mature earthworms were divided into 4 replicates for the test item concentrations. They had an individual body weight between 0.30 and 0.60 g.
After 28 days no biologically significant mortality was seen in the control and at all tested application rates (Table 1). Also, no pathological symptoms or changes in the behaviour of adult earthworms were seen in the control and at all tested application rates. The earthworm biomass increased in the control and at all tested application rates over 28 days without any statistically significant differences compared to the control. After further four weeks the reproduction rate (average number of juveniles) was 323 in the control and ranged between 265 and 347 at the test item application rates. The earthworm reproduction rate was not statistically significantly reduced compared to the control at 125, 250, 500 and 1000 mg/kg soil dry weight, whilst at the lowest concentration of 62.5 mg/kg soil dry weight a significant reduction was observed. Since this effect on earthworm reproduction only occurs at the lowest test item concentration, this effect is not considered to be a test item related effect (no dose response).
Overall, the NOEC of Tall oil diethylenetriamine imidazolineconcerning mortality, biomass and reproduction of earthworms was determined to be at 1000 mg/kg soil dry weight.
All validity criteria recommended by the test guidelines were fulfilled.
Summary of All Observed Effects in the Tested Doses
Effects
Tall oil diethylenetriamine imidazoline
mg/kg soil dry weight
62.5
125
250
500
1000
Mortality of adult earthworms
No
No
No
No
No
Reduction of body weight
No
No
No
No
No
Reduced reproduction rate
(Number of juveniles)
Yes*
No
No
No
No
Summary of all effects
No
No
No
No
No
*) = no test item related effect
NOEC and LOEC for Reproduction, Mortality and Body Weight
Tall oil diethylenetriamine imidazoline
Application rate
[mg/kg soil dry weight]
NOECreproduction, mortality, body weight
1000
LOECreproduction, mortality, body weight
> 1000
Referenceopen allclose all
Mortality of Adult Earthworms in [%] after 28 Days of Exposure
Application rate [mg/kg soil dry weight] |
Mortality[%] |
||||||||
Replicate |
Mean |
||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
Control |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
1.25 |
62.5 |
10 |
0 |
10 |
0 |
– |
5.00 |
|||
125 |
0 |
0 |
0 |
0 |
0.00 |
||||
250 |
0 |
0 |
0 |
0 |
0.00 |
||||
500 |
0 |
0 |
0 |
0 |
0.00 |
||||
1000 |
0 |
0 |
0 |
0 |
0.00 |
Earthworm Behaviour and Pathological Symptoms after 28 Days of Exposure
Application rate [mg/kg soil dry weight] |
Effect |
Replicate |
|||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
Control |
A) |
10/10 |
10/10 |
10/10 |
9/10 |
10/10 |
10/10 |
10/10 |
10/10 |
H) |
0/10 |
0/10 |
0/10 |
1/10 |
0/10 |
0/10 |
0/10 |
0/10 |
|
62.5 |
A) |
9/10 |
10/10 |
9/10 |
10/10 |
– |
|||
H) |
1/10 |
0/10 |
1/10 |
0/10 |
|||||
125 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
||||
250 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
||||
500 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
||||
1000 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
Body Weight Changes of the Adult Earthworms
Application rate |
Rep. |
Mean body weights per replicate |
Mean body weight change of earthworms |
|||||
Test start |
28 days |
Per replicate |
Mean±SD |
Sign.* |
||||
[g] |
[g] |
[g] |
[%] |
[g] |
[%] |
|||
Control |
1 |
0.500 |
0.675 |
0.18 |
35.0 |
0.15 ± 0.03 |
28.8 ± 5.31 |
– |
2 |
0.531 |
0.645 |
0.11 |
21.5 |
||||
3 |
0.490 |
0.625 |
0.14 |
27.6 |
||||
4 |
0.526 |
0.704 |
0.18 |
33.9 |
||||
5 |
0.511 |
0.655 |
0.14 |
28.2 |
||||
6 |
0.500 |
0.662 |
0.16 |
32.4 |
||||
7 |
0.490 |
0.593 |
0.10 |
21.0 |
||||
8 |
0.506 |
0.661 |
0.16 |
30.6 |
||||
62.5 |
1 |
0.531 |
0.710 |
0.18 |
33.7 |
0.18 ± 0.02 |
36.3 ± 3.42 |
No |
2 |
0.508 |
0.716 |
0.21 |
40.9 |
||||
3 |
0.491 |
0.671 |
0.18 |
36.7 |
||||
4 |
0.507 |
0.678 |
0.17 |
33.7 |
||||
125 |
1 |
0.513 |
0.690 |
0.18 |
34.5 |
0.15 ± 0.03 |
29.9 ± 6.82 |
No |
2 |
0.487 |
0.614 |
0.13 |
26.1 |
||||
3 |
0.509 |
0.696 |
0.19 |
36.7 |
||||
4 |
0.528 |
0.646 |
0.12 |
22.3 |
||||
250 |
1 |
0.528 |
0.671 |
0.14 |
27.1 |
0.16 ± 0.02 |
30.8 ± 4.72 |
No |
2 |
0.512 |
0.675 |
0.16 |
31.8 |
||||
3 |
0.494 |
0.677 |
0.18 |
37.0 |
||||
4 |
0.500 |
0.636 |
0.14 |
27.2 |
||||
500 |
1 |
0.503 |
0.636 |
0.13 |
26.4 |
0.16 ± 0.06 |
31.2 ± 11.9 |
No |
2 |
0.491 |
0.712 |
0.22 |
45.0 |
||||
3 |
0.525 |
0.616 |
0.09 |
17.3 |
||||
4 |
0.509 |
0.692 |
0.18 |
36.0 |
||||
1000 |
1 |
0.531 |
0.639 |
0.11 |
20.3 |
0.16 ± 0.04 |
31.8 ± 9.12 |
No |
2 |
0.504 |
0.719 |
0.22 |
42.7 |
||||
3 |
0.514 |
0.681 |
0.17 |
32.5 |
||||
4 |
0.488 |
0.643 |
0.16 |
31.8 |
Reproduction Rate (Number of Juveniles after 8 Weeks)
Application rate |
Rep. |
Number of juveniles |
Mean±SD |
CV [%] |
[%] of control |
Significance |
Control |
1 |
292 |
323 ± 27.2 |
8.43 |
– |
– |
2 |
285 |
|||||
3 |
332 |
|||||
4 |
299 |
|||||
5 |
353 |
|||||
6 |
355 |
|||||
7 |
332 |
|||||
8 |
334 |
|||||
62.5 |
1 |
227 |
265 ± 39.4 |
14.9 |
82.0 |
Yes |
2 |
260 |
|||||
3 |
252 |
|||||
4 |
320 |
|||||
125 |
1 |
316 |
301 ± 26.1 |
8.65 |
93.3 |
No |
2 |
322 |
|||||
3 |
302 |
|||||
4 |
264 |
|||||
250 |
1 |
350 |
347 ± 41.3 |
11.9 |
107 |
No |
2 |
296 |
|||||
3 |
397 |
|||||
4 |
343 |
|||||
500 |
1 |
310 |
307 ± 8.04 |
2.62 |
95.1 |
No |
2 |
316 |
|||||
3 |
305 |
|||||
4 |
297 |
|||||
1000 |
1 |
259 |
291 ± 29.8 |
10.2 |
90.2 |
No |
2 |
281 |
|||||
3 |
330 |
|||||
4 |
295 |
Mortality of Adult Earthworms in [%] after 28 Days of Exposure
Application rate [mg/kg soil dry weight] |
Mortality [%] |
||||||||
Replicate |
Mean |
||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
Control |
0 |
0 |
0 |
10 |
0 |
0 |
0 |
0 |
1.25 |
62.5 |
10 |
0 |
10 |
0 |
– |
5.00 |
|||
125 |
0 |
0 |
0 |
0 |
0.00 |
||||
250 |
0 |
0 |
0 |
0 |
0.00 |
||||
500 |
0 |
0 |
0 |
0 |
0.00 |
||||
1000 |
0 |
0 |
0 |
0 |
0.00 |
Earthworm Behaviour and Pathological Symptoms after 28 Days of Exposure
Application rate [mg/kg soil dry weight] |
Effect |
Replicate |
|||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
Control |
A) |
10/10 |
10/10 |
10/10 |
9/10 |
10/10 |
10/10 |
10/10 |
10/10 |
H) |
0/10 |
0/10 |
0/10 |
1/10 |
0/10 |
0/10 |
0/10 |
0/10 |
|
62.5 |
A) |
9/10 |
10/10 |
9/10 |
10/10 |
– |
|||
H) |
1/10 |
0/10 |
1/10 |
0/10 |
|||||
125 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
||||
250 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
||||
500 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
||||
1000 |
A) |
10/10 |
10/10 |
10/10 |
10/10 |
Body Weight Changes of the Adult Earthworms
Application rate |
Rep. |
Mean body weights per replicate |
Mean body weight change of earthworms |
|||||
Test start |
28 days |
Per replicate |
Mean ± SD |
Sign.* |
||||
[g] |
[g] |
[g] |
[%] |
[g] |
[%] |
|||
Control |
1 |
0.500 |
0.675 |
0.18 |
35.0 |
0.15 ± 0.03 |
28.8 ± 5.31 |
– |
2 |
0.531 |
0.645 |
0.11 |
21.5 |
||||
3 |
0.490 |
0.625 |
0.14 |
27.6 |
||||
4 |
0.526 |
0.704 |
0.18 |
33.9 |
||||
5 |
0.511 |
0.655 |
0.14 |
28.2 |
||||
6 |
0.500 |
0.662 |
0.16 |
32.4 |
||||
7 |
0.490 |
0.593 |
0.10 |
21.0 |
||||
8 |
0.506 |
0.661 |
0.16 |
30.6 |
||||
62.5 |
1 |
0.531 |
0.710 |
0.18 |
33.7 |
0.18 ± 0.02 |
36.3 ± 3.42 |
No |
2 |
0.508 |
0.716 |
0.21 |
40.9 |
||||
3 |
0.491 |
0.671 |
0.18 |
36.7 |
||||
4 |
0.507 |
0.678 |
0.17 |
33.7 |
||||
125 |
1 |
0.513 |
0.690 |
0.18 |
34.5 |
0.15 ± 0.03 |
29.9 ± 6.82 |
No |
2 |
0.487 |
0.614 |
0.13 |
26.1 |
||||
3 |
0.509 |
0.696 |
0.19 |
36.7 |
||||
4 |
0.528 |
0.646 |
0.12 |
22.3 |
||||
250 |
1 |
0.528 |
0.671 |
0.14 |
27.1 |
0.16 ± 0.02 |
30.8 ± 4.72 |
No |
2 |
0.512 |
0.675 |
0.16 |
31.8 |
||||
3 |
0.494 |
0.677 |
0.18 |
37.0 |
||||
4 |
0.500 |
0.636 |
0.14 |
27.2 |
||||
500 |
1 |
0.503 |
0.636 |
0.13 |
26.4 |
0.16 ± 0.06 |
31.2 ± 11.9 |
No |
2 |
0.491 |
0.712 |
0.22 |
45.0 |
||||
3 |
0.525 |
0.616 |
0.09 |
17.3 |
||||
4 |
0.509 |
0.692 |
0.18 |
36.0 |
||||
1000 |
1 |
0.531 |
0.639 |
0.11 |
20.3 |
0.16 ± 0.04 |
31.8 ± 9.12 |
No |
2 |
0.504 |
0.719 |
0.22 |
42.7 |
||||
3 |
0.514 |
0.681 |
0.17 |
32.5 |
||||
4 |
0.488 |
0.643 |
0.16 |
31.8 |
Reproduction Rate (Number of Juveniles after 8 Weeks)
Application rate |
Rep. |
Number of juveniles |
Mean ± SD |
CV [%] |
[%] of control |
Significance |
Control |
1 |
292 |
323 ± 27.2 |
8.43 |
– |
– |
2 |
285 |
|||||
3 |
332 |
|||||
4 |
299 |
|||||
5 |
353 |
|||||
6 |
355 |
|||||
7 |
332 |
|||||
8 |
334 |
|||||
62.5 |
1 |
227 |
265 ± 39.4 |
14.9 |
82.0 |
Yes |
2 |
260 |
|||||
3 |
252 |
|||||
4 |
320 |
|||||
125 |
1 |
316 |
301 ± 26.1 |
8.65 |
93.3 |
No |
2 |
322 |
|||||
3 |
302 |
|||||
4 |
264 |
|||||
250 |
1 |
350 |
347 ± 41.3 |
11.9 |
107 |
No |
2 |
296 |
|||||
3 |
397 |
|||||
4 |
343 |
|||||
500 |
1 |
310 |
307 ± 8.04 |
2.62 |
95.1 |
No |
2 |
316 |
|||||
3 |
305 |
|||||
4 |
297 |
|||||
1000 |
1 |
259 |
291 ± 29.8 |
10.2 |
90.2 |
No |
2 |
281 |
|||||
3 |
330 |
|||||
4 |
295 |
Description of key information
It is considered appropriate to address the data requirements for DTO_DETA by read-across to the available studies on the main components of DTO_DETA: AAI_DETA and Rosin.
The long-term earthworm study for AAI_DETA revealed a NOEC of 944.0 mg a.i./kg soil dw. No data were available for Rosin.
The AAI_DETA dataset is worst case with regards to biodegradability and aquatic toxicity, with the dataset for Rosin showing a lower toxicity profile. No data for the toxicity to soil macroorganisms are available for Rosin. Therefore, AAI_DETA is considered to be a worst case read-across source for the DTO_DETA target substance in the context of toxicity to soil macroorganisms. The toxicity of DTO_DETA to soil macroorganisms is therefore estimated to be a NOEC = 944.0 mg a.i./kg soil dw.
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 944 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.