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EC number: 257-479-6 | CAS number: 51858-17-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Dec 2017 - 12 Jan 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
Test material
- Reference substance name:
- Dipotassium dihydrogen 4-[5-[3-carboxylato-5-hydroxy-1-(4-sulphonatophenyl)-1H-pyrazol-4-yl]penta-2,4-dienylidene]-4,5-dihydro-5-oxo-1-(4-sulphonatophenyl)-1H-pyrazole-3-carboxylate
- EC Number:
- 257-479-6
- EC Name:
- Dipotassium dihydrogen 4-[5-[3-carboxylato-5-hydroxy-1-(4-sulphonatophenyl)-1H-pyrazol-4-yl]penta-2,4-dienylidene]-4,5-dihydro-5-oxo-1-(4-sulphonatophenyl)-1H-pyrazole-3-carboxylate
- Cas Number:
- 51858-17-4
- Molecular formula:
- C25H16N4O12S2.2K
- IUPAC Name:
- dipotassium dihydrogen 4-{5-[3-carboxylato-5-oxo-1-(4-sulfonatophenyl)-1,5-dihydro-4H-pyrazol-4-ylidene]penta-1,3-dien-1-yl}-5-hydroxy-1-(4-sulfonatophenyl)-1H-pyrazole-3-carboxylate
- Test material form:
- solid: crystalline
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Aerobic activated sludge from sewage treatment plant Rossdorf, Germany
- Storage conditions: aerated overnight
- Storage length: sludge was stored overnight
- Preparation of inoculum for exposure: Activated sludge was washed by centrifugation and the supernatant liquid phase was decanted; solid material was resuspended in test water and centrifuged again and repeated three times; based on the the ration of wet sludge to its dry weight calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated overnight.
- Pretreatment: An aliquot of sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined.
- Concentration of sludge: 3.5 g dry material per litre - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 102.9 mg/L
- Based on:
- test mat.
- Initial conc.:
- 107.1 mg/L
- Based on:
- ThOD
- Remarks:
- ThODNH4
- Initial conc.:
- 144.4 mg/L
- Based on:
- ThOD
- Remarks:
- ThODNO3
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according the OECD guideline 301
- Test temperature: 22 ± 1 °C
- pH: 7.2 - 7.4 at the end of the test; 8.0 at the beginning (adjusted to 7.6 with hydrochloric acid)
- pH adjusted: Yes
- Continuous darkness: Yes
- Other: Continuous stirring
TEST SYSTEM
- Culturing apparatus and measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany (Manometric Test System, 500 mL fill volume in flasks)
- Number of culture flasks/concentration: 2
- Details of trap for CO2: potassium hydroxide solution (45%)
SAMPLING
- Sampling method: Change of pressure in the test flasks was measured by means of a manometric method each day.
CONTROL AND BLANK SYSTEM
- Inoculum control: Yes, 2 replicates
- Abiotic sterile control: Yes,1 replicate (poisoned with HgCl2)
- Toxicity control: Yes,1 replicate
- Other: procedure control: Yes, 1 replicate
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of two replicates
- Details on results:
- The test item never reached 10% biodegradation. The mean biodegradation percentage at the end of the 28-day exposure period was 1% based on ThODNH4 (1% based on ThODNO3).The degradation rate of the test item never reached 60%. Therefore, Oxonole Blue is considered to be not readily biodegradable.
In the toxicity control 53% and 47% biodegradation was noted within 14 days and 60% as well as 53% biodegradation after 28 days of incubation (based on ThODNH4 and ThODNO3). According to the test guidelines, the test item can be assumed to be not inhibitory to the aerobic activated sludge microorganisms because degradation was >25% within 14 days.
BOD5 / COD results
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 77% after 14 days and to 85% after 28 days of incubation.
Any other information on results incl. tables
Table 1: Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control based on ThODNH4
Time |
Percentage Biodegradation1 |
|||
(Days) |
Oxonole Blue1 |
Sodium Benzoate 2 |
Toxicity |
|
|
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
1 |
0 |
0 |
32 |
23 |
2 |
0 |
0 |
41 |
29 |
3 |
0 |
0 |
49 |
34 |
4 |
0 |
0 |
56 |
41 |
5 |
0 |
0 |
64 |
43 |
6 |
0 |
0 |
68 |
48 |
7 |
0 |
0 |
69 |
49 |
8 |
0 |
0 |
72 |
49 |
9 |
0 |
2 |
74 |
50 |
10 |
0 |
0 |
72 |
51 |
11 |
0 |
0 |
75 |
51 |
12 |
0 |
5 |
75 |
52 |
13 |
0 |
5 |
75 |
52 |
14 |
0 |
2 |
77 |
53 |
15 |
0 |
2 |
77 |
55 |
16 |
0 |
2 |
80 |
55 |
17 |
0 |
0 |
78 |
54 |
18 |
0 |
5 |
78 |
54 |
19 |
0 |
2 |
77 |
55 |
20 |
0 |
2 |
77 |
55 |
21 |
0 |
2 |
80 |
57 |
22 |
0 |
2 |
80 |
59 |
23 |
0 |
2 |
80 |
59 |
24 |
0 |
2 |
80 |
59 |
25 |
0 |
2 |
80 |
59 |
26 |
0 |
2 |
82 |
59 |
27 |
0 |
2 |
82 |
59 |
28 |
0 |
2 |
85 |
60 |
1 ThODNH4 of Oxonole Blue: 1.041 mg O2/mg test item
2 ThODNH4 of sodium benzoate: 1.666 mg O2/mg reference item
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
2% |
yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
>60% |
yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
27.5 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- See details in section "Any other information on results incl tables"
- Interpretation of results:
- under test conditions no biodegradation observed
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