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EC number: 205-137-1 | CAS number: 134-31-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Quinolin-8-ol
- EC Number:
- 205-711-1
- EC Name:
- Quinolin-8-ol
- Cas Number:
- 148-24-3
- Molecular formula:
- C9H7NO
- IUPAC Name:
- Quinolin-8-ol
Constituent 1
Method
- Target gene:
- Salmonella typhimunum strains TA97, TA98, TA100, TA1535, and TA1537 were obtained from Dr. Bruce Ames (University of California, Berkeley) and were stored as recommended [Maron and Ames, 1983]. Cultures were grown overnight with shaking at 37°C in Oxoid No. 2 broth, and their phenotypes were analyzed prior to their use for mutagenicity assays.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- CELLS USED
- Source of cells: University of California, Berkley (Dr. Bruse Ames)
- Suitability of cells:
- Cell cycle length, doubling time or proliferation index: cultures were grown overnight with shaking at 37oC in Oxoid No.2 broth.
- Sex, age and number of blood donors if applicable:
- Whether whole blood or separated lymphocytes were used if applicable:
- Number of passages if applicable:
- Methods for maintenance in cell culture if applicable:
- Modal number of chromosomes:
- Normal (negative control) cell cycle time:
MEDIA USED
- Type and identity of media including CO2 concentration if applicable:
- Properly maintained: [yes/no]
- Periodically checked for Mycoplasma contamination: [yes/no]
- Periodically checked for karyotype stability: [yes/no)
- Periodically 'cleansed' against high spontaneous background: [yes/no]
- Species / strain / cell type:
- S. typhimurium TA 97
- Details on mammalian cell type (if applicable):
- CELLS USED
- Source of cells: University of California, Berkley (Dr. Bruse Ames)
- Suitability of cells:
- Cell cycle length, doubling time or proliferation index: cultures were grown overnight with shaking at 37oC in Oxoid No.2 broth.
- Sex, age and number of blood donors if applicable:
- Whether whole blood or separated lymphocytes were used if applicable:
- Number of passages if applicable:
- Methods for maintenance in cell culture if applicable:
- Modal number of chromosomes:
- Normal (negative control) cell cycle time:
MEDIA USED
- Type and identity of media including CO2 concentration if applicable:
- Properly maintained: [yes/no]
- Periodically checked for Mycoplasma contamination: [yes/no]
- Periodically checked for karyotype stability: [yes/no)
- Periodically 'cleansed' against high spontaneous background: [yes/no]
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water, DMSO, 95% ethanol, acetone
Each laboratory made an independent assessment of the solvents to be used. Tests were performed at Microbiological Associates,
Inc. (MIC) and SFU International (NU);on e chemical was also tested at Case Western Reserve University (CWR). A number of chemicals were tested in more than one laboratory or at different times in the same laboratory. The laboratory repeating the test was not informed of the identity of the chemical or that it had been tested previously
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- in the absence of metabolic activation were sodium azide (TA1535 and TA 100)
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- in the absence of metabolic activation (TA97 and TA 1537)
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylenediamine
- Remarks:
- in the absence of metabolic activation (TA98)
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- positive control for metabolic activation with all strains
- Negative solvent / vehicle controls:
- yes
- Remarks:
- cuncurrent solvent: distilled water, DMSO, 95% ethanol, acetone
- Remarks:
- Concurrent solvent and positive controls were run with each trial
- Details on test system and experimental conditions:
- The preincubation assay was performed as here described: the test chemical (0.05 ml), Salmonella culture (0.10 ml), and S-9 mix or buffer (0.50 ml) were incubated at 37°C, without shaking, for 20 min. The top agar was added and the contents of the tubes were mixed and poured onto the surface of petri dishes containing Vogel-Bonner medium [Vogel and Bonner, 1956]. The histidine-independent (his+) colonies arising on these plates were counted following two days incubation at 37°C. Plates were machine counted.
The test item was tested initially in a toxicity assay to determine the appropriate dose range for the mutagenicity assay.
Four variations of protocol were used:
1)Testing in strains TA97, TA98, TA100, and TA1535, with some additional testing in strain TA1537; 10% S-9 was used.
2) The first test of the chemical was without activation and with 10% S-9 in the S-9 mix. If a positive result was obtained the test was repeated. If the tests were negative they were repeated without S-9 and with 30% S-9.
3) The order of use of 10% and 30% S-9 was reversed.
4) Initial testing was in strains TA98 and TA100 without activation and with 30% rat and hamster S-9s. If a positive result was obtained in one of these two strains it was repeated and the other strains were not used. If the tests were negative, the other strains were used with 30% and 10% S-9. A chemical was not designated non-mutagenic unless it had been tested in strains TA98, TA100, TA1535, and TA97 and/or TA1537, without activation and with 10% and 30% rat and hamster S9. Occasionally, 5% S-9 was also used in all protocol variations. - Evaluation criteria:
- Individual trials were judged mutagenic (+), weakly mutagenic (+ W), questionable (?), or nonmutagenic (-), depending on the magnitude of the increase of his+ revertants, and the shape of the dose-response. A trial was considered questionable (?) if the dose-response was judged insufficiently high to support a call of “ +W,” if only a single dose was elevated over the control, or if the increase seen was not dose related.
Results and discussion
Test results
- Species / strain:
- not specified
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The test item produced mutagenic responses.
- Executive summary:
In this published review three hundred chemicals were tested for mutagenicity (Ames test) in Salmonella typhimurium, using a preincubation protocol by the U.S. National Toxicology Program. All tests were performed by many indipendent laboratories in the absence of exogenous metabolic activation, and in the presence of liver S-9 from Aroclor-induced male Sprague-Dawley rats and Syrian hamsters.
The test item was tested in Microbial Genetics Department, SRI International, Menlo Park, California (K. M.) and produced mutagenic responses.
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