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EC number: 948-260-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- 1. The oxygen content measurement time was lower and higher than 8 minutes 2. The addition of the sludge to the test assay with reference substance RS6 was done accidentally 15 minutes before the scheduled time
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of (4Z,8E)-dodeca-4,8,11-trienal and (4E,8Z)-dodeca-4,8,11-trienal
- EC Number:
- 701-295-5
- Molecular formula:
- C12 H18 O
- IUPAC Name:
- Reaction mass of (4Z,8E)-dodeca-4,8,11-trienal and (4E,8Z)-dodeca-4,8,11-trienal
Constituent 1
- Specific details on test material used for the study:
- Name of test substance: Dodecatrienal
Test substance No.: 18/0072-2
Batch identification: V15 Trienal-Dest. F.4-7+V16 Trienal-Dest.F4-8.
CAS no.: 1000399-21-2
Purity: 90.1 area-% (GC, RTX-1701 capillary); 92.7 area-% (GC, DB-35 MS capillary)
Homogeneity: Homogeneous
Storage stability: Expiry date: May 2020
Date of production: May 2018
Physical state/
Appearance:
liquid / colorless, clear
Water solubility: 43 mg/L +/- 4 mg/L at T = 20.0°C +/- 0.5 °C
Storage conditions: Ambient at room temperature; under light exclusion
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test substance was added in the required amounts according to the test concentrations directly to the test vessels with 234 mL deionized water. Aliquots of the reference substance stock solution were dosed to the test vessels and made up with deionized water to a volume of 234 mL. 16 mL synthetic medium were dosed to each test vessel with test substance and reference substance afterwards. To prepare the blank control assays 234 mL of deionized water and 16 mL synthetic medium were mixed. The pH-values in all test vessels were checked. An adjustment was not necessary. After addition of 250 mL of inoculum suspension (3 g/L DW) the incubation was started by aeration of the test vessels with pressured air. The vessels for the blank control assays were prepared to the same procedure without addition of test- or reference substance. After 3 hours incubation at 20 ± 2°C the mixtures in the test vessels were placed subsequently for oxygen measuring. The temperature was measured for seven times in a separate vessel filled with aerated deionized water during incubation phase.
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Test system: municipal Activated sludge
Reason for the selection of the test system: required by the test guidelines
Age: 1 day
Origin: aeration tank of the wastewater treatment plant of Mannheim, Germany
Collection of the test system: 04 Sep 2018
Arrival in the test facility: 04 Sep 2018
After arrival of the activated sludge suspension in the test facility the suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with drinking water and the suspension was adjusted to 3 g/L Dw.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20.3 – 20.6°C
- pH:
- 7.2 - 8.2
- Dissolved oxygen:
- 7.0 - 9.0 (start of exposure)
- Nominal and measured concentrations:
- 1000, 500, 250, 125 and 62.5 mg/L as nominal concentration based on test substance
- Details on test conditions:
- Test replicates: 6 replicates for the control, 3 replicates for each test substance concentration, 2 replicates for each reference substance concentration.
Incubation time: 3 hours
Test vessels: Glas-beakers (nominal volume 1L)
Test volume: 500 mL
Synthetic medium: 16 mL/test vessel of 100-fold concentrated OECD medium
Oxygen concentration during aeration: > 2 mg/L
Oxygen concentration immediately before measurement: > 7 mg/L
Duration of the measurement of oxygen consumption: About 8 - 10 min (two deviations as described above)
Sludge concentration in the test vessel: 1.5 g/L Dw
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- EC10 = 49 mg/L, EC20 = 72 mg/L, EC50 = 150 mg/L, EC80 = 320 mg/L were found in the activated sludge respiration inhibition test with 3 hour residence time for the test substance Dodecatrienal.
Validity criteria:
The coefficient of variation of the six replicates of blank control was 10.6 % O2 consumption.
The EC50 of 3,5-dichlorophenol was 4.3 mg/L.
The mean oxygen uptake of the blank controls was 21 mg/g*h.
In conclusion, the test conformed to all validity criteria and is valid. - Results with reference substance (positive control):
- In order to verify that the microorganisms are responding normally to toxic stress, tests with a reference substance are conducted according to OECD 209 guideline. The results from the reference substance test are compared to EC50 values published in OECD 209 guideline, which represent the typical response range.
According to the test guideline OECD 209 the EC50 values of the reference substance 3,5-dichlorophenol should be in the range of 2-25 mg/L after 3 hours of incubation. The EC50 of the reference substance 3,5-dichlorophenol was in the range of 2-25 mg/L in 3
hours. These results indicate that the microorganisms are responding normally to toxicant stress. - Reported statistics and error estimates:
- The consumption rates were used for the determination of the ECx by the probit method based on Finney with the software TOXRAT Professional 2.10. The effect concentrations were given with an accuracy of 2 significant digits.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion the 3 hours effect concentrations for Dodecatrienal in the Activated Sludge
Respiration Inhibition Test are:
Toxicity Response Variable Based on nominal effect concentration [mg/L] Confidence Limits (95%) lower/upper
EC10 49 9.9/80.6
EC20 72 23.3/106.8
EC50 150 99.1/222.8
EC80 320 215.6/908.5
The value of effect concentrations of EC10 EC20 EC50 EC80 were given with an accuracy of 2
significant digits. The degree of inhibition was evaluated by Probit analysis according to Finney.
The results in this study are consistent with all validity criteria and the test is valid according to
the guidelines used for this study.
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