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EC number: 232-339-7 | CAS number: 8005-52-5 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 29000.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13.12.2002 - 18.07.2003
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: method No. L 45: “Acute Toxicity for Daphnia, immobilization test. Chemical formulations and waste waters”
- Deviations:
- no
- Principles of method if other than guideline:
- Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. The sensitivity of the test species is periodically monitored by testing with the reference substance, potassium dichromate.The sample is dissolved in the dilution water at a concentration of about 1.0 g / L and during the test formed a fine precipitate that gradually settled at the bottom. The volume of the test mixture for one dilution is 50 ml.Preliminary test was carried on with 10 organisms for every concentrations and control, a limit test was carried on with 3x10 organisms and two controls. The limit test is repeated 2x. Immobilization is monitored at 24 and 48 hours. Solutions are not aerated. The temperature of the solution during the test is 20.0 ± 1.0 °C. The oxygen content and pH values are measured in each vessel at the beginning and end of the test.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on test solutions:
- The stock solution of the test substance was prepared in the dilution water. 1000 mg of the test substance was weighed into 1000 mL of the dilution water for the preliminary test.The concentrations of solutions used in test were obtained by dilution of the stock solution with dilution water. The stock solution for the preliminary test: 1.0 g·L-1 The stock solution for the limit test No.1 and 2: 1.0 g·L-1
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna Straus were obtained by aperiodic parthenogenesis from own laboratory breeding. Daphnia were fed by a mixture of algae.The grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. The sensitivity of the test species and correctness of test performance is periodically verified by testing with the reference substance, potassium dichromate.TEST ORGANISM- Common name: Daphnia Magna- Strain: Straus- Source: own laboratory breeding- Age at study initiation: young daphnia born in 24 hours- Method of breeding: aperiodic parthenogenesis- Feeding during test: noACCLIMATIONThe grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. - Type and amount of food: a mixture of algae- Health during acclimation: healthy
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 20.0 ± 1.0 °CLimit tests at 0; 48 hoursLimit test No. 1: 19.5; 20.0 °CLimit test No. 1: 19.2; 20.1 °C
- pH:
- Limit test No. 1 at 0; 48 hours: pH of the controls: 7.98 – 7.78pH of the tested concentrations: 8.04 – 7.79Limit test No. 2 at 0; 48 hours: pH of the controls: 7.96 – 7.76pH of the tested concentrations: 7.99 – 7.81
- Dissolved oxygen:
- Limit test No. 1: Oxygen content in the control: 8.8 mg·L-1Oxygen content in the tested concentrations: 8.8 mg·L-1Limit test No. 2 at 0; 48 hours:Oxygen content in the control: 8.9; 8.8 mg·L-1Oxygen content in the tested concentrations: 8.9; 8.8 mg·L-1
- Nominal and measured concentrations:
- Preliminary test: 1000, 800, 600, 400, 200 mg/L + controlFull test 1 and 2: 3x 1000 mg/L + 2x control
- Details on test conditions:
- TEST SYSTEM- Test vessel: glass beakers (volume of the test solution is 50ml in each test beaker)- Type (delete if not applicable): open- Aeration: no- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 3- No. of vessels per control (replicates): 2Preliminary Test: 10 animals for every concentration + controlLimit test No.1: 3x10 animals + 2 controlsLimit test No.2: 3x10 animals + 2 controlsDilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1 000 mL of deionized water.A. 117.6 g CaCl2.2H20 in 1 L of deionized waterB. 49.3 g MgSO4.7H2O in 1 L of deionized waterC. 25.9 g NaHCO3 in 1 L of deionized waterD. 2.3 g KCl in 1 L of deionized waterpH 7.8, saturated with oxygen at T= 20 - 24 °C
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Executive summary:
The test substance, Saturnová žluť L4G 75 (Direct Yellow 44), was tested in acute immobilisation test on Daphnia magna.
The test was performed as static.
Test result: EC50 (48h) > 1000 mg·L-1
Reference
The test quality criteria
These quality criteria given in the current guideline were fulfilled.
1. Immobilisation in the controls must not exceed 10 % at the end of the test (there were no immobilisation)
2. The dissolved oxygen concentrations (throughout the test) should remain above 3 mg·L-1
Description of key information
48 h - EC50 > 1000.0 mg.L-1
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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