Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 244-479-6 | CAS number: 21615-47-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Mar 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 2000 mg test item (corresponding to 1000 mg a.i.) was accurately weighed and was filled up to 100 mL with purified water. A stock solution of 10000 mg a.i./L in the test item was obtained.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: no
- Name and location of sewage treatment plant where inoculum was collected: Obtained on 27 Mar 2017 from an aeration reactor of a sewage treatment plant (Kurume central sewage treatment center (Kurume-shi, Fukuoka, Japan)
- Preparation of inoculum for exposure: 5 L of activated sludge was fed with the synthetic sewage (250 mL) and cultivated for 21.5 h at 20 ± 2 °C under aerobic conditions in order to acclimate to the test temperature.
- Suspended solids concentration: 2910 mg/L; 10 mL of the activated sludge was filtrated and the residue was dried for 2 h at 110 °C and weighed (in accordance with Japanese Industrial Standards (JIS) K 0102:2016, Section 14.1) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.5 (reference substance and blank control)
7.1 (test solutions) - Nominal and measured concentrations:
- nominal: control, 1000 mg a.i./L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL glass vessels
- Material, size, headspace, fill volume: Glass, 300 mL, headspace: 50 mL, fill volume: 250 mL
- Aeration: 0.5 - 1 L/min
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per reference mixture (replicates): 1
- Nutrients provided for bacteria: synthetic sewage (according to guideline)
- Nitrification inhibitor used: none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
OTHER TEST CONDITIONS
- Adjustment of pH: The pH was adjusted to 7.5 witm 1 mol/L sodium hydroxide solution since the pH of thereference mixture and blank control was < 7.0.
- Details on termination of incubation: After 3 h of exposure, the measurement bottles were filed with the test solution with attention to avoid foaming. Afterwards the dissolved oxygen was measured continuously for 10 min.
EFFECT PARAMETERS MEASURED
- O2 consumption was measured at the end of 3 h incubation period.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not relevant, limit test
- Justification for using fewer concentrations than requested by guideline: No inhibition of respiration was recorded in the range-finding test. Thus, a limit test is justified.
- Range finding study
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: 1% inhibition at 10 mg/L, 11% inhibition at 100 mg/L, 12% inhibition at 1000 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate: 47.8, 49.2, 47.7 mg O2/L/h (at test start); 45.9, 44.4, 42.9 mg O2/L/h (at test end)
- Coefficient of variation of oxygen uptake rate in control replicates: 5.9% - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3 h): 11 mg/L - Reported statistics and error estimates:
- In the reference mixture, the percentage inhibition in each exposure levels was plotted on semi logarithmic graph against the corresponding concentration. The EC50 of 3,5-dichlorophenol was calculated from the regression formula obtained by probit analysis. The NOEC of the test item was estimated by the following statistical analyses. Regarding the oxygen consumption rate, F- test was done to determine the homogeneity of variance for the data, Aspin-Welch t-test was used to estimate the significant difference in comparison with the control.
- Validity criteria fulfilled:
- yes
Reference
Table 1: Test results
|
Nominal concentration [mg/L] |
Oxygen consumption rate [mg O2/L/h] |
Percentage inhibition of respiration [%] |
Average percentage inhibition [%] |
Test substance |
1000 |
46.3, 47.9, 48.0 |
0, -3, -4 |
-2 |
Reference substance |
4.0 |
39.4 |
15 |
- |
8.0 |
29.3 |
37 |
- |
|
16 |
13.5 |
71 |
- |
|
32 |
6.76 |
85 |
- |
|
Blank control (test start) |
- |
47.8; 49.2; 47.7 |
- |
- |
Blank control (test end) |
45.9; 44.9; 42.9 |
Description of key information
EC50 (3 h) > 1000 mg a.i./L (nominal; OECD 209)
NOEC (3 h) ≥ 1000 mg a.i./L (nominal; OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
One experimental study according to OECD 209 (GLP) is available investigating the effects of ammonium undecafluorohexanoate (CAS 21615-47-4) on respiration of activated sludge microorganisms. The study was performed as a limit test with a nominal concentration of 1000 mg a.i./L. Activated sludge from a sewage treatment plant treating predominantly domestic sewage was used as inoculum. After incubation for 3 h the oxygen consumption rate was measured in the control and exposure vessels. Since no significant inhibition compared to the control was recorded the EC50 (3 h) was derived to be > 1000 mg a.i./L and the NOEC (3 h) as ≥ 1000 mg a.i./L. Thus, the substance is not expected to inhibit the activity of activated sludge microorganisms in sewage treatment plants.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.