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Diss Factsheets
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EC number: 200-463-0 | CAS number: 60-23-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- adopted July 17,1992
- GLP compliance:
- yes
Test material
- Reference substance name:
- Mercaptamine
- EC Number:
- 200-463-0
- EC Name:
- Mercaptamine
- Cas Number:
- 60-23-1
- Molecular formula:
- C2H7NS
- IUPAC Name:
- mercaptamine
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The freshly obtained sludge was kept under continuous aeration until further treatment
- Pretreatment: Before use, the sludge was allowed to settle (35 minutes) and the supernatant liquid was used as inoculum at the amount of 10 mL/L of mineral medium.
- Concentration of sludge: The concentration of suspended solids was determined to be 3.2 g/L in the concentrated sludge. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 1.14 other: mg CO2 mg
- Based on:
- ThCO2
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: standard under the guideline
- Test temperature: 22.2 - 23.2°C
- pH: recorded on Day 0 and Day 28 for blanks and test item (Day 14 for positive control); pH adjusted in test item and toxicity control bottles
- Aeration of dilution water: A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The synthetic air was passed through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: Test suspension: containing test item and inoculum (2 bottles); Inoculum blank: containing only inoculum (2 bottles); Positive control: containing reference item and inoculum (1 bottle).; Toxicity control: containing test item, reference item and inoculum (1 bottle).
- Method used to create aerobic conditions: the synthetic air was passed through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
- Test performed in open system: Yes
- Details of trap for CO2: Three CO2-absorbers (bottles filled with 100 mL 0.0125 M Ba(OH)2) were connected in series to the exit air line of each test bottle.
SAMPLING
- Sampling frequency and method : Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until day 28, for the inoculum blank and test item. Titrations for the positive and toxicity control were made over a period of at least 14 days.
Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers were moved one position in the direction of the test bottle. A new CO2-absorber was placed at the far end of the series. Phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator.
On the penultimate day, the pH of respective test suspensions was measured and 1 mL of concentrated HCl (37%, Merck) was added to the bottles of the inoculum blank and test suspension. The bottles were aerated overnight to drive off CO2 present in the test suspension. The final titration was made on day 15 (positive and toxicity control) and on day 29 (remaining vessels).
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
STATISTICAL METHODS: not applicable
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
- Preliminary study:
- not applicable
- Test performance:
- This study was considered to be valid.
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 5
- Sampling time:
- 28 d
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period revealed no biological relevant biodegradation of Mercaptamine (3% and 7%, based on ThCO2).
In the toxicity control, more than 25% biodegradation occurred within 14 days (38%, based on ThCO2). Therefore, the test item was assumed not to inhibit microbial activity.
BOD5 / COD results
- Results with reference substance:
- 80% degradation after Day 14. Validity criteria met.
Any other information on results incl. tables
Table1 pH Values of Different Test Media
Test medium: |
At the start of the test: |
On day 14: |
On day 28: |
Blank control (A) |
7.6 |
- |
7.7 |
Blank control (B) |
7.6 |
- |
7.7 |
Positive control |
7.6 |
7.9 |
- |
Mercaptamine (A) |
7.9 → 7.61 |
- |
7.8 |
Mercaptamine (B) |
8.0 → 7.61 |
- |
7.7 |
Toxicity control |
7.9 → 7.61 |
7.9 |
- |
¹: Adjusted using 1 M HCl (Merck, Darmstadt, Germany)
Table 2: Comparison of Biodegradation of the Test Item in Bottles A and B
Day |
Biodegradation (%) |
|||
Bottle A |
Bottle B |
Mean A and B |
∆ A-B1) |
|
1 |
0 |
1 |
1 |
1 |
4 |
1 |
1 |
1 |
0 |
6 |
1 |
2 |
2 |
1 |
8 |
1 |
3 |
2 |
2 |
11 |
1 |
3 |
2 |
2 |
15 |
1 |
4 |
3 |
3 |
18 |
1 |
4 |
3 |
3 |
22 |
1 |
5 |
3 |
4 |
25 |
2 |
5 |
4 |
3 |
292) |
2 |
6 |
4 |
4 |
292) |
2 |
7 |
5 |
5 |
292) |
3 |
7 |
5 |
4 |
1): Absolute difference in biodegradation between bottles A and B 2): Biodegradation is ended on day 28 by addition of HCl. Therefore, differences observed on day 29 are actually differences of day 28. |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- In conclusion, Mercaptamine was not readily biodegradable under the conditions of the modified Sturm test
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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