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EC number: 207-894-3 | CAS number: 499-83-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- the deviations can be considered uncritical
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Pyridine-2,6-dicarboxylic acid
- EC Number:
- 207-894-3
- EC Name:
- Pyridine-2,6-dicarboxylic acid
- Cas Number:
- 499-83-2
- Molecular formula:
- C7H5NO4
- IUPAC Name:
- pyridine-2,6-dicarboxylic acid
- Test material form:
- solid: crystalline
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution containing 100.2 mg/L test item in algal medium (demineralised water enriched with minerals but without algae) was prepared. Lower test concentrations were prepared by dilution of the stock solution in algal medium.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.7 – 23.5 °C
- pH:
- ca. 8.8
- Nominal and measured concentrations:
- 1 / 3.2 / 10 / 32 / 100 mg/L nominal concentration
The measured concentrations lay between 92 % and 125 % of the nominal concentrations in all treatments respectively between 92 % and 104 % in the concentrations to be used for evaluation. Therefore, the determination of the results was based on the nominal concentrations. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 107.63 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: extrapolated
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 43.98 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Any other information on results incl. tables
Two experiments were performed. In the 1stexperiment one of the validity criteria was not met. Therefore, the experiment was repeated. The results of the 1stexperiment are not stated in the report, but will be kept together with the other raw data in the GLP archive of the test facility.
The study was performed using 5 concentrations ranging from 1.0 to 100 mg/L. Incubation time (test systemDesmodesmus subspicatus)was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield[1] were determined from the cell number at the respective observation times.
In the 3 highest concentrated treatments a clear dose response relationship was observed. However in the 2 lowest concentrated treatments the cell number was strong scattering and in some replicated the algal cells were agglomerated. This effect was not caused by the test item because in the next highest test concentration no inhibition of algal growth was observed. Therefore, the two lowest treatments were not used for statistical evaluation.
At the start and at the end of the test, the content of the test item in the test solutions was determined using HPLC.
The measured concentrations lay between 92 % and 125 % of the nominal concentrations in all treatments respectively between 92 % and 104 % in the concentrations to be used for evaluation. Therefore, the determination of the results was based on the nominal concentrations.
The 72h-EC50s of potassium dichromate were determined in a separate reference test. For the estimation of the 72h-EC50s of the positive control, the fits showed sufficient statistical correspondence of the data with the dose-response-equation. The values were within the range of the laboratory.
The pH of the blank control should not fluctuate by more than 1.5 units. The change was 0.3 units in the blank control.
All validity criteria were met.
No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid.
[1]Yield (according to OECD Guideline 201) is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period. Calculation see under 8.2 Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. Algal biomass is defined as the dry weight per volume, e.g. mg algae/L test solution. However, dry weight is difficult to measure and therefore surrogate parameters are used. Of these surrogates, cell counts are most often used.
Parameter |
Value |
95% confidence interval |
NOEC (Growth Rate) 72 h |
10.00 mg/L |
-- |
NOEC (Yield) 72 h |
10.00 mg/L |
-- |
LOEC (Growth Rate) 72 h |
32.00 mg/L |
-- |
LOEC (Yield) 72 h |
32.00 mg/L |
-- |
72h ErC10 |
43.98 mg/L |
39.44 – 49.05 mg/L |
72h EyC10 |
26.03 mg/L |
21.15 – 32.02 mg/L |
72h ErC50 |
107.63 mg/L (extrapolated) |
93.37 – 122.88 mg/L |
72h EyC50 |
50.28 mg/L |
39.80 – 63.45 mg/L |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Endpoint NOEC LOEC EC10 EC50
Growth Rate 10.00 mg/L 32.00 mg/L 43.98 mg/L 107.63 mg/L
(extrapolated)
Yield 10.00 mg/L 32.00 mg/L 26.03 mg/L 50.28 mg/L - Executive summary:
Two experiments were performed. In the 1stexperiment one of the validity criteria was not met. Therefore, the experiment was repeated. The results of the 1stexperiment are not stated in the report, but will be kept together with the other raw data in the GLP archive of the test facility.
The study was performed using 5 concentrations ranging from 1.0 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield[1] were determined from the cell number at the respective observation times.
In the 3 highest concentrated treatments a clear dose response relationship was observed. However in the 2 lowest concentrated treatments the cell number was strong scattering and in some replicated the algal cells were agglomerated. This effect was not caused by the test item because in the next highest test concentration no inhibition of algal growth was observed. Therefore, the two lowest treatments were not used for statistical evaluation.
At the start and at the end of the test, the content of the test item in the test solutions was determined using HPLC.
The measured concentrations lay between 92 % and 125 % of the nominal concentrations in all treatments respectively between 92 % and 104 % in the concentrations to be used for evaluation. Therefore, the determination of the results was based on the nominal concentrations.
The 72h-EC50s of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
[1]Yield (according to OECD Guideline 201) is defined as the biomass at the end of the exposure period minus the biomass at the start of the exposure period. Calculation see under 8.2 Growth and growth inhibition are quantified from measurements of the algal biomass as a function of time. Algal biomass is defined as the dry weight per volume, e.g. mg algae/L test solution. However, dry weight is difficult to measure and therefore surrogate parameters are used. Of these surrogates, cell counts are most often used.
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