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EC number: 220-543-9 | CAS number: 2802-68-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- see attached justification
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- read-across source
Reference
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: ATCC-22662
- Source (laboratory, culture collection): National Institute Environmental Research, Korea
- Age of inoculum (at test initiation): 7 days
- Method of cultivation: The flasks then were placed on a shaker incubator at 170 rpm and 25°C, with 24 h light supplied via worm white fluorescent tubes, with an average illumination of 30 ± 5µE/m2s.
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not): Stock alga was cultivated in 250 mL Erlenmeyer flasks, containing 200 mL sterilized nitrate-enriched BBM medium prepared in triple distilled water, to avoid nitrogen limitation during the high-density culture. The culture flask was agitated on a shaker at 170 rpm, and bubbled with air (1 vvm), without sparger. Light was continuously supplied, with an average of 30 ±5 µE/m2s, using warm-white fluorescent tubes located on top of the shaker. All the flasks were maintained in the shaker incubator at 25 ± 5°C for 7 days. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 25°C
- Details on test conditions:
- TEST SYSTEM
Experiments were performed in 250 mL Erlenmeyer flasks containing 55 mL of sterilized culture medium, inoculated with 5 mL samples of 7 day cultured algae. Solutions of organic solvents were subsequently added to the test flasks. The organic solvents tested were completely miscible with water, forming a homogeneous phase. Concentrations were adjusted in the range between 0.1 M and 1.26 µM to obtain complete concentration-response curves. At each determined exposure date, the optical density of the algal biomass was estimated at 438 nm using a spectrophotometer. The dry cell weight, corresponding to the optical density, was determined from the linear relationship; dry cell weight (g/L) = 0.329 x optical density. All experiments were performed in duplicate, but the control experiments were conducted in triplicate. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 22 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Reason / purpose for cross-reference:
- read-across source
Reference
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- from 2004-06-28 to 2005-06-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
A stock solution of the test item diluted in water was prepared one hour before the beginning of the test by mixing 500 mg of boron trifluoride dihydrate in 1 L of dilution water. Volumetric flasks were filled with adequate volumes of the test item stock solution and dilution water in order to obtain the test concetrations. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4
- Source: Culture Centre of Algae and Protozoa, Ambleside, UK
- Culture conditions: 23 ± 1 °C, photoperiod 16 h illumination, 8 h darkness, continuous aeration (0.22 µm filter)
- Stock cultures are renewed every two weeks
PRECULTURE
- Period: 4 days prior to test initiation
- Culturing media same as test
- Age of inoculum (at test initiation): 4 days - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 ± 1 °C
- pH:
- 7.7 - 9.7
- Dissolved oxygen:
- 8.8 - 9.8 mg/L
- Nominal and measured concentrations:
- Nominal: 0, 102, 173, 294, 500 mg/L
Recoveries 91 - 100 % - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL erlenmeyer flasks stoppered with cotton wool wrapped in sterilised gauze
- Test volume: 50 mL
- Initial cells density: 10000 cells/mL
- Control end cells density: 1051000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes (C3)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: as described in method C.3. of directive 92/69/EEC
- Culture medium same as test medium
- Intervals of water quality measurement: pH and dissolved oxygen after 0 and 72 h
OTHER TEST CONDITIONS
- Photoperiod: constant illumination
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: using an optical microscope equipped with an haemacytometer (Malassez cell counter), recorded daily
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.7
- Range finding study: 0.1 - 100 mg/L, no effects observed - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 294 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 294 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 294 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 173 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - The increase in cell density (R), measured in the control solution between the beginning and the end of the test, was greater than a factor of 16 (R = 105).
- Microscopic observations confirmed that the algal cells appeared normal at the end of the test - Results with reference substance (positive control):
- - ErC50 = 0.93 mg/L; EbC50 = 0.34 mg/L (obtained 07-2004)
- Reason / purpose for cross-reference:
- read-across source
Reference
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Test type:
- static
- Limit test:
- no
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: Element/Boron
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 52.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- other: Element/Boron
- Basis for effect:
- growth rate
Data source
Materials and methods
Test material
- Reference substance name:
- Hydrogen trifluoromethoxyborate(1-), compound with methanol (1:1)
- EC Number:
- 220-543-9
- EC Name:
- Hydrogen trifluoromethoxyborate(1-), compound with methanol (1:1)
- Cas Number:
- 2802-68-8
- Molecular formula:
- CH4O.CH3BF3O.H
- IUPAC Name:
- hydrogen trifluoro(methanolato)borate(1-) methanol (1:1)
- Details on test material:
- Name of the test substance used in the study report: Borfluorid-Methanol
Constituent 1
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- growth rate
- Remarks on result:
- other: Value derived from effect concentrations observed in test with boric acid (value expressed as mg B/L recalculated accounting for molecular weight of the target substance)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- growth rate
- Remarks on result:
- other: Value derived from effect concentrations observed in test with boric acid (value expressed as mg B/L recalculated accounting for molecular weight of the target substance)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.