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EC number: 226-901-0 | CAS number: 5538-94-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 June to 27 August 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- A preliminary study was conducted to determine the approximate degradation rate and evaluate the test substance prior to defining the definitive test. The preliminary test was run using buffer solutions at pH 5, 7 and 9. At pH 7 both 'HEPES' and 'TRIS' buffers were tested. The nominal test concentration was 10 ug/mL Both silanised and non-silanised test vessels were used to evaluate adsorption. The test vessels were wrapped in foil to exclude light, placed on a mechanical shaker in an environmental chamber maintained at 25+/-1oC. Samples were taken after 0, 1, 4, 8, 21 and 96 hours for analysis by liquid scintillation counting (LSC) and thin-layer chromatography (TLC).
A 30-day hydrolysis study with C14 didecyldimethylammonium chloride was conducted at 25oC at pH 5, 7 and 9. All experiments were conducted at the nominal test concentration of 10 ug/mL under sterile conditions and in darkness. Samples were taken at 0, 4, 7, 14, 22 and 30 days and were quantified for test substance and hydrolysis products by liquid scintillation counting (LSC) and thin-layer chromatography (TLC). - GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Supplied by Lonza Inc., batch/lot no. 7499-E
- Expiration date of the lot/batch: No data
- Purity test date: No data
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity: 5.49 x 10*7 dpm/mL
- Locations of the label: on an N-methyl group
- Expiration date of radiochemical substance: No data
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
OTHER SPECIFICS: - Radiolabelling:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Preliminary study samples were taken after 0, 1, 4, 8, 21 and 96 hours
Definitive study samples were taken after 0, 4, 7, 14, 22 and 30 days
Duplicate aliquots from each sample were analysed by LSC and TLC. - Buffers:
- - pH: 5
Addition of 59.2 mL 0.2M acetic acid to 140.8mL of 0.2M sodium acetate. The solution was dluted to 400mL in water
- pH: 7 (TRIS)
Addiition of 94mL of 0.2M hydrochliric acid to 100mL of 0.2M tris(hydroxymethyl)aminomethane. The solution was diluted to 400mL in water
- pH: 7 (HEPES)
Addition of 2.38g N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES) to 1000mL water (0.01M), then adding 2M potassium hydroxide until pH7 was achieved
- pH: 9
Addition of 100mL of 0.2M boric acid to 118mL of 0.2Mborax solution. The solution was diluted to 400mL with water.
All water used to prepare buffers was Millipore Milli-Q purified, filtered through 0.2um filter. - Preliminary study:
- A preliminary test using pH 5, 7 and 9 buffers was conducted over 96 hours and indicated that DDAC was stable under the conditions of the test.
- Transformation products:
- not measured
- pH:
- 5
- Remarks on result:
- hydrolytically stable based on preliminary test
- pH:
- 7
- Remarks on result:
- hydrolytically stable based on preliminary test
- pH:
- 9
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 5
- Temp.:
- 30 °C
- DT50:
- >= 368 d
- Type:
- not specified
- Key result
- pH:
- 7
- Temp.:
- 30 °C
- DT50:
- >= 175 d
- Type:
- not specified
- Key result
- pH:
- 9
- Temp.:
- 30 °C
- DT50:
- >= 506 d
- Type:
- not specified
- Validity criteria fulfilled:
- not specified
- Conclusions:
- A preliminary test using pH 5, 7 (HEPES & TRIS buffer) and 9 buffers over 96 hours indicated that DDAC was stable under the conditions of the test. It was also determined that the test substance did not adsorb to either silanised or non-silanised glass.
A definitive test using pH 5, 7 (HEPES & TRIS buffer) and 9 buffers over 30 days gave calculated half-lives of 368, 175, 194 and 506 days, respectively - Executive summary:
A hydrolysis study was conducted according to the principles of GLP but did not follow a specific test guideline. A preliminary test using pH 5, 7 and 9 buffers was conducted over 96 hours and indicated that DDAC was stable under the conditions of the test.
A definitive test using pH 5, 7 and 9 buffers was conducted over 30 days. Calculated half-lives of 368 (pH5), 175 (pH7), 194 (pH7) and 506 (pH9) days indicated the stability of the test substance to hydrolysis. TLC analysis demonstrated a decrease in C14 activity of less than 10% in the parent test substance.
The test substance is hydrolytically stable at pHs 5, 7 and 9 at 25oC under the conditions of the test.
Reference
Preliminary test results
Sampling time (h) |
pH 5 (preliminary test) |
|
|||
Measured concn, non‑silanised (ug/mL) |
Measured conc, silanised (ug/mL) |
Mean measured conc (ug/mL) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
|
0 |
9.09 |
9.09 |
9.09 |
100 |
95.7 |
1 |
9.06 |
8.39 |
8.73 |
96 |
97.2 |
4 |
9.23 |
9.38 |
9.31 |
102 |
96.0 |
8 |
9.36 |
8.90 |
9.13 |
100 |
95.5 |
21 |
10.2 |
8.75 |
9.48 |
104 |
92.8 |
96 |
8.47 |
10.2 |
9.34 |
103 |
- |
Sampling time (h) |
pH 7 HEPES buffer (preliminary test) |
pH 7 TRIS buffer (preliminary test) |
||||||||
Measured concn, non‑silanised (ug/mL) |
Measured conc, silanised (ug/mL) |
Mean measured conc (ug/mL) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
Measured concn, non‑silanised (ug/mL) |
Measured conc, silanised (ug/mL) |
Mean measured conc (ug/mL) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
|
0 |
9.77 |
9.77 |
9.77 |
100 |
93.9 |
8.56 |
8.56 |
8.56 |
100 |
93.6 |
1 |
8.58 |
8.56 |
8.57 |
88 |
97.4 |
8.56 |
8.73 |
8.65 |
101 |
97.9 |
4 |
8.71 |
8.93 |
8.82 |
90 |
95.2 |
8.56 |
8.67 |
8.62 |
101 |
95.1 |
8 |
9.13 |
9.58 |
9.36 |
96 |
88.5 |
8.43 |
8.25 |
8.34 |
97 |
96.1 |
21 |
9.38 |
9.19 |
9.29 |
95 |
88.0 |
8.64 |
8.68 |
8.66 |
101 |
97.0 |
96 |
9.77 |
9.80 |
9.79 |
100 |
- |
9.57 |
8.30 |
8.94 |
104 |
- |
Sampling time (h) |
pH 9 (preliminary test) |
||||
Measured concn, non‑silanised (ug/mL) |
Measured conc, silanised (ug/mL) |
Mean measured conc (ug/mL) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
|
0 |
10.0 |
10.0 |
10.0 |
100 |
92.6 |
1 |
8.69 |
8.92 |
8.81 |
88 |
97.9 |
4 |
8.48 |
9.17 |
8.83 |
88 |
95.2 |
8 |
8.58 |
8.89 |
8.74 |
87 |
89.5 |
21 |
9.42 |
9.80 |
9.61 |
96 |
93.8 |
96 |
9.81 |
9.81 |
9.81 |
98 |
- |
Definitive test results
Sample day |
pH 5 (definitive test) |
|||
Measured concn (ug/mL as DDAC) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
Mass balance (% of initial total activity) |
|
0 |
7.98 |
100 |
92.8 |
100 |
4 |
8.26 |
104 |
92.7 |
104 |
7 |
8.23 |
103 |
92.0 |
104 |
14 |
7.98 |
100 |
92.8 |
100 |
22 |
8.13 |
102 |
95.9 |
98.6 |
30 |
7.56 |
94.8 |
92.0 |
95.6 |
Sample day |
pH 5 HEPES buffer (definitive test) |
pH 5 TRIS buffer (definitive test) |
||||||
Measured concn (ug/mL as DDAC) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
Mass balance (% of initial total activity) |
Measured concn (ug/mL as DDAC) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
Mass balance (% of initial total activity) |
|
0 |
7.98 |
100 |
94.2 |
100 |
8.33 |
100 |
95.9 |
100 |
4 |
8.47 |
106 |
93.9 |
106 |
8.32 |
99.9 |
93.5 |
102 |
7 |
7.89 |
98.9 |
92.4 |
101 |
8.09 |
97.1 |
93.1 |
100 |
14 |
7.90 |
99.0 |
94.5 |
98.7 |
8.30 |
99.6 |
95.8 |
100 |
22 |
7.45 |
93.4 |
94.0 |
93.6 |
7.67 |
92.0 |
97.9 |
90.1 |
30 |
7.45 |
93.4 |
89.9 |
97.9 |
7.52 |
90.3 |
94.5 |
91.6 |
Sample day |
pH 9 (definitive test) |
|||
Measured concn (ug/mL as DDAC) |
% recovery of initial measured concn |
TLC (% 14C as DDAC) |
Mass balance (% of initial total activity) |
|
0 |
8.28 |
100 |
95.5 |
100 |
4 |
7.88 |
95.2 |
92.0 |
98.8 |
7 |
7.80 |
94.2 |
93.5 |
96.2 |
14 |
8.12 |
98.0 |
95.7 |
97.8 |
22 |
7.74 |
93.4 |
96.7 |
92.3 |
30 |
7.80 |
94.1 |
94.6 |
95.0 |
Description of key information
There are no studies available for the determination of hydrolysis of the substance. However, there is a reliable study (Klimisch 2) available for the read-across substance, didecyldimethyl ammonium chloride (DDAC). A definitive test was conducted over 30 days at pH 5, 7 and 9. The calculated half-lives were all greater than 175 days indicating the test substance was hydrolytically stable under the conditions of the test. Supporting thin-layer chromatography (TLC) analysis indicated less than 10% decrease in C14 activity in the parent test substance. This study was conducted according to the principles of GLP but did not follow a specific test guideline.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 175 d
- at the temperature of:
- 25 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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