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EC number: 265-352-1 | CAS number: 65060-02-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 04 April, 2012 to 26 April, 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Test substance was added to the incubation vessels using a stock solution of approximately 1.0 g/L.The stock solution of quaternary ammonium compounds, C12-14 (evennumbered)-alkylethyldimethyl, ethyl sulphates was prepared by dissolving 0.78 g C12-14 alkyl ethyl dimethyl ethyl sulphates QAS in 780 mL deionized water.
pH stock solution C12-14 alkyl ethyl dimethyl ethyl sulphates QAS =6.6 - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Unadapted secondary activated sludge was obtained (24-04-2012) from the WWTP Nieuwgraaf in Duiven. The WWTP Duiven is an activated sludge plant treating predominantly domestic wastewater. Prior to use the activated sludge was homogenized with a syringe. The dry weight of the homogenized activated sludge was determined and subsequently concentrated by settlement to the required dry weight concentration. The dry weight of the activated sludge in the incubation vessels was 1.5 g/L.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- Temperature varied from 19.8 to 20.6°C.
- pH:
- The pH of the reaction mixtures after the incubation period ranged from 7.2 to 7.4.
- Dissolved oxygen:
- Aerobic during test
- Nominal and measured concentrations:
- Test substance: 10 mg/L, 20 mg/L, 40 mg/L, 80 mg/L and 160 mg/L (nominal)
Reference compound 3,5-dichlorophenol: 2.5 mg/L, 5.0 mg/L,10.0 mg/L, 20.0 mg/L and 40.0 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 30 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: with 95% confidence limits of 13 and 72 mg/L
- Details on results:
- Test conditions
The pH of the reaction mixtures after the incubation period ranged from 7.2 to 7.4. Temperature varied from 19.8 to 20.6°C. These conditions allow
respiration of the activated sludge used.
Validity of the test
The validity of the test is demonstrated by three criteria. First, the coefficient of variation of the replicates of the control oxygen uptake rates was 10%, which fulfils the maximum prescribed variation of <30%. Secondly, the prescribed average oxygen uptake rate of > 20 mg O2/g dry weight/hour for the control measurements was achieved (30 mg O2/g dry weight/hour). The third criterion was met as shown by the EC50 of the reference compound of 15 mg/L, which is within the prescribed range of 2 to 25 mg/L.
Activated sludge respiration inhibition test
The inhibitory effect of test substance at a particular concentration is expressed as a percentage of the two controls. From the results EC values were calculated. The EC50 of test substance for activated sludge after 3 hours contact time is 30 mg/L with 95% confidence limits of 13 and 72 mg/L. The EC10, EC20 and EC80 are 9, 14 and 66 mg/L, respectively. - Results with reference substance (positive control):
- The EC50 of the reference compound was 15 mg/L at a contact time of 3 h, respectively. These EC50 values is within the prescribed range of 2 to 25 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the EC50 of test substance in sludge after 3 h of contact time was determined to be 30 mg a.i./L (nominal).
- Executive summary:
A study was conducted to determine the toxicity to microorganisms of the test substance, C12-14 ADMAES (96% active), in an activated sludge respiration inhibition testaccording to OECD 209 Guideline, in compliance with GLP. The toxicity of the test substance to activated sludge was determined at a contact time of 3 h, using various concentrations of the test substance. The inhibitory effect of the substance at a particular concentration is expressed as a percentage of the two controls. No analytical determination was performed for the test substance. The EC50 of test substance in sludge after 3 h of contact time was determined to be 30 mg/L (nominal) (equiavalent to 28.8 mg a.i./L) (95% confidence limits: 13 and 72 mg/L). The 3 h EC10, EC20, EC80 were determined to be 9, 14 and 66 mg/L (nominal) (equiavalent to 8.64, 13.44 and 63.36 mg a.i./L), respectively. The test substance was therefore considered harmful to activated sludge. The test was valid as shown by the EC50 of the reference compound 3,5 -dichlorophenol (15 mg/L), the coefficient of variation of the replicates of the control oxygen uptake rates (10%) and the average oxygen uptake rate for the control replicates (30 mg O2/g dry weight/hour). Under the study conditions, the EC50 of test substance in sludge after 3 h of contact time was determined to be 30 mg a.i./L (nominal) (Geerts, 2012).
Reference
Results
Table I Respiration rates of the activated sludge, inhibition percentages, pH and temperature at various concentrations of 3,5-dichlorophenol after 3 hours contact time
Concentration (mg/L) |
Activity (mgO2/g/min) |
Inhibition (%) |
pH at start test |
pHatend test |
Temperature at end test (°C) |
Control |
0.5222 |
- |
7.0 |
7.2 |
19.8 |
Control |
0.5222 |
- |
7.1 |
7.2 |
20.0 |
Control* |
0.4556 |
- |
7.1 |
7.2 |
20.2 |
Control* |
0.4556 |
- |
7.1 |
7.3 |
20.4 |
2.5 |
0.5000 |
4 |
7.1 |
7.3 |
20.1 |
5.0* |
0.3778 |
17 |
7.1 |
7.2 |
20.0 |
10.0 |
0.3444 |
34 |
7.1 |
7.3 |
20.0 |
20.0 |
0.1500 |
71 |
7.0 |
7.3 |
20.2 |
40.0 |
0.1143 |
78 |
7.0 |
7.3 |
20.4 |
Concentration stock solution 3,5-dichlorophenol = 999 mg/L pH stock solution 3,5-dichlorophenol = 7.1
* = corresponding control and reference concentration
Table II Respiration rates of the activated sludge, inhibition percentages, pH and temperature at various concentrations of test substance after 3 hours contact time.
Concentration (mg/L) |
Activity (mgO2/g/min) |
Inhibition (%) |
pH at start test |
pHatend test |
Temperature end test (°C) |
Control |
0.6133 |
- |
7.1 |
7.3 |
20.2 |
Control |
0.5333 |
- |
7.1 |
7.3 |
20.6 |
Control* |
0.5278 |
- |
7.1 |
7.4 |
20.5 |
Control* |
0.4889 |
- |
7.2 |
7.3 |
20.3 |
5* |
0.5048 |
1 |
7.1 |
7.3 |
20.4 |
10 |
0.4714 |
18 |
7.1 |
7.3 |
20.2 |
20 |
0.3444 |
40 |
7.2 |
7.3 |
20.0 |
40 |
0.2519 |
56 |
7.2 |
7.4 |
20.3 |
80 |
0.0778 |
86 |
7.2 |
7.4 |
20.1 |
160 |
0.0238 |
96 |
7.2 |
7.4 |
20.3 |
Concentration stock solution test substance = 1 g/L
pH stock solution of test substance = 6.6
* = corresponding control and reference concentration
Description of key information
Based on the results of the read across study, the 3 h EC50 and EC10 values for toxicity in micro-organisms is considered to be 30 and 9 mg a.i./kg; these values have been considered further for hazard/risk assessment.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 30 mg/L
- EC10 or NOEC for microorganisms:
- 9 mg/L
Additional information
A study was conducted to determine the toxicity to microorganisms of the read across substance, C12-14 ADMAES (96% active), in an activated sludge respiration inhibition testaccording to OECD 209 Guideline, in compliance with GLP. The toxicity of the read across substance to activated sludge was determined at a contact time of 3 h, using various concentrations of the read across substance. The inhibitory effect of the substance at a particular concentration is expressed as a percentage of the two controls. No analytical determination was performed for the read across substance. The EC50 of read across substance in sludge after 3 h of contact time was determined to be 30 mg/L (nominal) (equivalent to 28.8 mg a.i./L) (95% confidence limits: 13 and 72 mg/L). The 3 h EC10, EC20, EC80 were determined to be 9, 14 and 66 mg/L (nominal) (equivalent to 8.64, 13.44 and 63.36 mg a.i./L), respectively. The read across substance was therefore considered harmful to activated sludge. The test was valid as shown by the EC50 of the reference compound 3,5 -dichlorophenol (15 mg/L), the coefficient of variation of the replicates of the control oxygen uptake rates (10%) and the average oxygen uptake rate for the control replicates (30 mg O2/g dry weight/hour). Under the study conditions, the EC50 of read across substance in sludge after 3 h of contact time was determined to be 30 mg a.i./L (nominal) (Geerts, 2012). Based on the results of the read across study, similar effect levels can be considered for the test substance.
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