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Diss Factsheets
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EC number: 210-498-3 | CAS number: 616-91-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-04-10 to 2018-06-09
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Duplicate samples from the freshly prepared test medium of the test concentration and the control were taken at the start of the test. For the determination of the stability of the test item under the test conditions and of the maintenance of the test item concentration during the test period, duplicate samples from the test medium of the test concentration and the control were collected at the end of the test (after 48 hours).
- Sample storage conditions before analysis: All samples were stored in a freezer (< - 20 °C), protected from light, until analysis was performed. Afterwards the samples were again stored deep frozen (< -20 °C) and will be kept stored up to the date of the final report. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 100 mg/L was prepared by dissolving 40 mg test item into 400 mL test water by intense stirring for 10 minutes.
- Controls: test water only
- Evidence of undissolved material: no - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna (Straus), clone 5
- Source: in-house laboratory culture
- Age of parental stock: 1.75 - 19.75 hours
- Feeding during test: no
- Breeding conditions: bred under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids was performed in Elendt M4 medium. The test organisms were not first brood progeny. Daphnids in stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus)
ACCLIMATION: was not necessary, since the test was performed in the same medium as the culturing - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
- Hardness:
- 2.5 mmol/L (= 250 mg/L) as CaCO3
- Test temperature:
- 19.9 - 20.4 °C
- pH:
- 6.3 to 8.0 at test start; 7.6 to 7.8 at test end
- Dissolved oxygen:
- 9.2 mg/L at test start; 8.8 mg/L at test end
- Salinity:
- Not applicable
- Conductivity:
- Not available
- Nominal and measured concentrations:
- Nominal: 100 mg/L
Measured: 100% of nominal (100 mg/L) - Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Material, size, fill volume, lid: glass, 100 mL nominal, filled with approximately 60 mL test medium, covered with lid
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: tes media were prepared with deionised water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h dark
- Light intensity: 670 to 820 lux
EFFECT PARAMETERS MEASURED: mobility after 24 and 48 hours
RANGE-FINDING STUDY (non-GLP)
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium hydrogen phthalate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities: no
- Observations on body length and weight: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- Relevant effect levels: EC50
- Limit test: no
- Dose-response test: yes
- EC50 (48 hours): 1.02 mg/L - Reported statistics and error estimates:
- NOEC and LOEC were determined directly from the raw data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a Immobilisation Test with Daphnia magna according to OECD TG 202 and EU Method C.2, the 48-hour EC50 of the test item was determined to be >100 mg/L.
- Executive summary:
The toxic effect of the test item to Daphnia magna was assessed in a static limit test according to OECD TG 202, EU Method C.2 and GLP principles. Young daphnids were exposed for 48 hours to the test item concentration of 100 mg/L (5 animals per vessel, 4 replicates). In parallel a negative control (test water only) was performed (5 animals per vessel, 4 replicates). The suitability of the test item was confirmed by a positive control with the reference compound potassium hydrogen phthalate. The test item concentration was verified by analysis of Total Organic Carbon to be 100% of the nominal concentration. Therefore, biological results are based on nominal test item concentration.The 48-hour EC50 was determined to be >100 mg/L. The 48-hour NOEC was determined to be at least 100 mg test item/L. The NOEC might even be higher than this concentration, but concentrations in excess of nominal 100 mg test item/L have not been tested according to the recommendations of the test guidelines. The 48-hour LOEC was higher than 100 mg test item/L. These values could not be quantified due to the absence of toxicity of the test item up to nominal 100 mg/L. All validity criteria of the guidelines were fulfilled.
Reference
Description of key information
In an Acute Immobilisation Test with Daphnia magna according to OECD TG 202 and EU Method C.2, the 48-hour EC50 of the test item was determined to be >100 mg/L (reference 6.1.3 -1).
Key value for chemical safety assessment
Additional information
The toxic effect of the test item to Daphnia magna was assessed in a static limit test according to OECD TG 202, EU Method C.2 and GLP principles. Young daphnids were exposed for 48 hours to the limit test item concentration of 100 mg/L (5 animals per vessel, 4 replicates). In parallel a negative control (test water only) was performed (5 animals per vessel, 4 replicates). The suitability of the test item was confirmed by a positive control with the reference compound potassium hydrogen phthalate. The test item concentration was verified by analysis of Total Organic Carbon to be 100% of the nominal concentration. Therefore, biological results are based on nominal test item concentration.The 48-hour EC50 was determined to be >100 mg/L. The 48-hour NOEC was found to be at least 100 mg test item/L. The NOEC might even be higher than this concentration, but concentrations in excess of nominal 100 mg test item/L have not been tested according to the recommendations of the test guidelines. The 48-hour LOEC was higher than 100 mg test item/L. These values could not be quantified due to the absence of toxicity of the test item up to nominal 100 mg/L. All validity criteria of the guidelines were fulfilled (reference 6.1.3 -1).
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