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Reaction mass of N,N-ethanediylbis- Hexadecanamide, N-[2-[(1-oxohexadecyl)amino]ethyl]-Octadecanamide, Hexadecanoic acid, 12-hydroxystearic acid, reaction products with ethylenediamine, N,N-ethanediylbis-Octadecanamide and Octadecanoic acid, 12-hydroxystearic acid, reaction products with ethylenediamine
EC number: 906-763-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening tests
- Type of information:
- calculation (if not (Q)SAR)
- Remarks:
- Migrated phrase: estimated by calculation
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientifically acceptable calculation
- Principles of method if other than guideline:
- BIOWIN v4.10
- Details on inoculum:
- BIOWIN v4.10 calculation
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: The micro-organisms were taken from different sources.
- Details on inoculum:
- Inoculum from specially grown non adapted micro-organisms.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 40 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days.
- Executive summary:
The ready biodegradation in the Modified MITI Test was determined with a non adapted inoculum for the test item over a period of 28 days. The study was conducted according to OECD guideline 301 C. The test item concentration selected as appropriate was 40 mg/L, corresponding to a ThOD of 108 mgO2/L in the test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation is therefore expressed as the percentage BOD and was calculated for each study day.
The oxygen depletion in the inoculum control came to 0.0 mgO2/L after 28 days.
In order to check the activity of the test system sodium benzoate was used as functional control. The functional control reached the pass level of a biodegradation > 40 % after 1 day and > 65 % biodegradation was reached after 3 days. After 17 days the degradation came to a maximum of 94 %.
The test item did not reach the 10 % level (beginning of biodegradation) until day 28. The biodegradation remained at a level of 0 – 4 %. The mean biodegradation came to 2 % after 28 days.
The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 February 1991- 22 April 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of activated sludge: Municipal sewage treatment plant, Schijndel, The Netherlands
- Preparation of inoculum: activated sludge aerated for 4 h and then left to settle at least for 1/2 h. The supernatant was decanted to provide a sufficiently large volume for a 1% inoculum for each test flask - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 20 +/- 2°C
- pH: pH blank, 6.2; pH positive control, 5.8; pH test substance 10mg/L, 5.9; pH test substance 20mg/L, 6.0
TEST SYSTEM
- Culturing apparatus: 3L bottles all-glass brown filled with 3L test medium
- Number of culture flasks/concentration: 1
- Method used to create aerobic conditions: aeration with CO2 free air at a constant rate of 50-100 mL air/min
- Details of trap for CO2: after the aeration period, three CO2-absorber bottles were filled with 80 ml 0.025 N Ba(OH)2 and connected in series to the exit air line of each test bottle.
SAMPLING
- Sampling frequency: 2,5,7,9,12,16,21,28 d
- Sampling method: CO2 was determined by titration of remaining Ba(OH)2 with 0.05 N standardized HCL, after barium carbonate precipitation (from
the reaction between CO2 and barium hydroxide). Periodically the C02—absorber nearest to the test bottle was removed for titration. The remaining
two absorbers were each moved one position in the direction of the test bottle, and a new absorber filled with 80 ml of 0025 N Ba(OH)2 (normalized
the same day) was placed at the far end of the series. All samples were stored at —20°C for three months after delivery of this report.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Reference compound: 1
- Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2.1
- Sampling time:
- 12 d
- Remarks on result:
- other: 10 mg/L test substance
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 15
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 mg/L test substance
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0.7
- Sampling time:
- 12 d
- Remarks on result:
- other: 20 mg/L test substance
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 5.5
- Sampling time:
- 28 d
- Remarks on result:
- other: 20 mg/L test substance
- Details on results:
- The test substance is not readily biodegradable under the tested conditions. The test substance was not degraded over 60% on a 10-day window
(days 2-12) for any of the tested concentrations (10 and 20 mg/L) - Results with reference substance:
- 103.5% sodium acetate was biodegraded by day 28. Degradation of >60% of reference substance was reached within 14 days
- Interpretation of results:
- other: not readily biodegradable as < 16% degradation under test conditions
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 24 Oct - 23 Dec 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: basic data given
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- yes
- Remarks:
- test duration was 2 weeks
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Details on inoculum:
- Concentration of sludge: 30 ppm- Duration of test (contact time):
- 14 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST SYSTEM
- Measuring equipment: Coulometer
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
STATISTICAL METHODS: not stated - Parameter:
- % degradation (O2 consumption)
- Value:
- 1.1
- Sampling time:
- 14 d
- Details on results:
- not biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to chapter 13
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 15
- Sampling time:
- 28 d
- Executive summary:
The ready biodegradation in the Modified MITI Test was determined with a non adapted inoculum for the test item over a period of 28 days. The study was conducted according to OECD guideline 301 C. The test item concentration selected as appropriate was 40 mg/L, corresponding to a ThOD of 108 mgO2/L in the test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation is therefore expressed as the percentage BOD and was calculated for each study day.
The oxygen depletion in the inoculum control came to 0.0 mgO2/L after 28 days.
In order to check the activity of the test system sodium benzoate was used as functional control. The functional control reached the pass level of a biodegradation > 40 % after 1 day and > 65 % biodegradation was reached after 3 days. After 17 days the degradation came to a maximum of 94 %.
The test item did not reach the 10 % level (beginning of biodegradation) until day 28. The biodegradation remained at a level of 0 – 4 %. The mean biodegradation came to 2 % after 28 days.
The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days.
Two additional studies investigating the ready biodegradability of the read Across Source substance Amides, C16-C18 (even), N,N'-ethylenebis were available. The report from Coenen (1991) was chosen as key study. This test was performed following the OECD Guideline No. 301B: Ready Biodegradability: Modified Sturm Test (1981), under GLP conditions. Activated sludge was used as inoculum and test material concentrations were 10 and 20 mg/L. On day 28 (end of the test) biodegradation values reached 15% and 5.5% at 10 and 20 mg/L test concentrations respectively. An additional supporting study was obtained from the Japanese Ministry of International Trade and Industry (MITI, 1976). This ready biodegradability test was carried out according to the OECD Guideline TG 301C: MITI (I), using activated sludge as inoculum at a concentration of 30 ppm. The test concentration used in the study was 100 ppm. After 14 days (end of the study), the test substance reached a biodegradation value of 1.1%. Thus, these results support those from the key study and Amides, C16-C18 (even), N,N'-ethylenebis can be considered as not readily biodegradable but the substance is hardly soluble in water and the very low solubility of the test substance may reduce the availability of the substance in the test medium.
Therefore, an additional calculation was used to investigate the biodegradation of Amides, C16-C18 (even), N,N'-ethylenebis. A QSAR prediction using the following models Biowin1, Biowin2, Biowin3, Biowin4, Biowin5, Biowin6 and Biowin7 shows that the substance is not persistent in the environment and the biodegradation takes place in a time frame of weeks.
Referenceopen allclose all
BIOWIN (v4.10) Program Results:
==============================
SMILES : O=C(NCCNC(=O)CCCCCCCCCCCCCCCCC)CCCCCCCCCCCCCCCCC
CHEM : Octadecanamide, N,N -1,2-ethanediylbis-
MOL FOR: C38 H76 N2 O2
MOL WT : 593.04
--------------------------- BIOWIN v4.10 Results ----------------------------
Biowin1 (Linear Model Prediction) : Biodegrades Fast
Biowin2 (Non-Linear Model Prediction): Biodegrades Fast
Biowin3 (Ultimate Biodegradation Timeframe): Weeks-Months
Biowin4 (Primary Biodegradation Timeframe): Days
Biowin5 (MITI Linear Model Prediction) : Biodegrades Fast
Biowin6 (MITI Non-Linear Model Prediction): Biodegrades Fast
Biowin7 (Anaerobic Model Prediction): Does Not Biodegrade Fast
Ready Biodegradability Prediction: NO
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin1 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.1084 | 0.2169
Frag | 2 | Amide [-C(=O)-N or -C(=S)-N] | 0.2102 | 0.4203
MolWt| * | Molecular Weight Parameter | | -0.2823
Const| * | Equation Constant | | 0.7475
============+============================================+=========+=========
RESULT | Biowin1 (Linear Biodeg Probability) | | 1.1024
============+============================================+=========+=========
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin2 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 1.8437 | 3.6874
Frag | 2 | Amide [-C(=O)-N or -C(=S)-N] | 2.6913 | 5.3826
MolWt| * | Molecular Weight Parameter | | -8.4212
============+============================================+=========+=========
RESULT | Biowin2 (Non-Linear Biodeg Probability) | | 0.9749
============+============================================+=========+=========
A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast
A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin3 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2983 | 0.5967
Frag | 2 | Amide [-C(=O)-N or -C(=S)-N] | -0.0542 | -0.1084
MolWt| * | Molecular Weight Parameter | | -1.3105
Const| * | Equation Constant | | 3.1992
============+============================================+=========+=========
RESULT | Biowin3 (Survey Model - Ultimate Biodeg) | | 2.3769
============+============================================+=========+=========
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin4 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Linear C4 terminal chain [CCC-CH3] | 0.2691 | 0.5381
Frag | 2 | Amide [-C(=O)-N or -C(=S)-N] | 0.2054 | 0.4109
MolWt| * | Molecular Weight Parameter | | -0.8556
Const| * | Equation Constant | | 3.8477
============+============================================+=========+=========
RESULT | Biowin4 (Survey Model - Primary Biodeg) | | 3.9411
============+============================================+=========+=========
Result Classification: 5.00 -> hours 4.00 -> days 3.00 -> weeks
(Primary & Ultimate) 2.00 -> months 1.00 -> longer
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin5 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Amide [-C(=O)-N or -C(=S)-N] | 0.1266 | 0.2533
Frag | 2 | Methyl [-CH3] | 0.0004 | 0.0008
Frag | 34 | -CH2- [linear] | 0.0494 | 1.6801
MolWt| * | Molecular Weight Parameter | | -1.7643
Const| * | Equation Constant | | 0.7121
============+============================================+=========+=========
RESULT | Biowin5 (MITI Linear Biodeg Probability) | | 0.8821
============+============================================+=========+=========
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin6 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Amide [-C(=O)-N or -C(=S)-N] | 0.8859 | 1.7717
Frag | 2 | Methyl [-CH3] | 0.0194 | 0.0389
Frag | 34 | -CH2- [linear] | 0.4295 | 14.6028
MolWt| * | Molecular Weight Parameter | |-17.1204
============+============================================+=========+=========
RESULT |Biowin6 (MITI Non-Linear Biodeg Probability)| | 0.8604
============+============================================+=========+=========
A Probability Greater Than or Equal to 0.5 indicates --> Readily Degradable
A Probability Less Than 0.5 indicates --> NOT Readily Degradable
------+-----+--------------------------------------------+---------+---------
TYPE | NUM | Biowin7 FRAGMENT DESCRIPTION | COEFF | VALUE
------+-----+--------------------------------------------+---------+---------
Frag | 2 | Linear C4 terminal chain [CCC-CH3] | -0.3177 | -0.6355
Frag | 2 | Amide [-C(=O)-N or -C(=S)-N] | -0.5679 | -1.1358
Frag | 2 | Methyl [-CH3] | -0.0796 | -0.1591
Frag | 34 | -CH2- [linear] | 0.0260 | 0.8837
Const| * | Equation Constant | | 0.8361
============+============================================+=========+=========
RESULT | Biowin7 (Anaerobic Linear Biodeg Prob) | | -0.2106
============+============================================+=========+=========
A Probability Greater Than or Equal to 0.5 indicates --> Biodegrades Fast
A Probability Less Than 0.5 indicates --> Does NOT Biodegrade Fast
Ready Biodegradability Prediction: (YES or NO)
----------------------------------------------
Criteria for the YES or NO prediction: If the Biowin3 (ultimate survey
model) result is "weeks" or faster (i.e. "days", "days to weeks", or
"weeks" AND the Biowin5 (MITI linear model) probability is >= 0.5, then
the prediction is YES (readily biodegradable). If this condition is not
satisfied, the prediction is NO (not readily biodegradable). This method
is based on application of Bayesian analysis to ready biodegradation data
(see Help). Biowin5 and 6 also predict ready biodegradability, but for
degradation in the OECD301C test only; using data from the Chemicals
Evaluation and Research Institute Japan (CERIJ) database.
Table 1. Result of biodegradability
|
Degradability |
Result by O2 consumption |
1.1% |
Result by HPLC |
(-) % |
Description of key information
The ready biodegradation in the Modified MITI Test was determined with a non adapted inoculum for the test item over a period of 28 days. The study was conducted according to OECD guideline 301 C. The test item concentration selected as appropriate was 40 mg/L, corresponding to a ThOD of 108 mgO2/L in the test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation is therefore expressed as the percentage BOD and was calculated for each study day.
The oxygen depletion in the inoculum control came to 0.0 mgO2/L after 28 days.
In order to check the activity of the test system sodium benzoate was used as functional control. The functional control reached the pass level of a biodegradation > 40 % after 1 day and > 65 % biodegradation was reached after 3 days. After 17 days the degradation came to a maximum of 94 %.
The test item did not reach the 10 % level (beginning of biodegradation) until day 28. The biodegradation remained at a level of 0 – 4 %. The mean biodegradation came to 2 % after 28 days.
The test item must be regarded as not readily biodegradable in the 10-d-window and after 28 days.
Two additional studies investigating the ready biodegradability of the read Across Source substance Amides, C16-C18 (even), N,N'-ethylenebis were available. The report from Coenen (1991) was chosen as key study. This test was performed following the OECD Guideline No. 301B: Ready Biodegradability: Modified Sturm Test (1981), under GLP conditions. Activated sludge was used as inoculum and test material concentrations were 10 and 20 mg/L. On day 28 (end of the test) biodegradation values reached 15% and 5.5% at 10 and 20 mg/L test concentrations respectively. An additional supporting study was obtained from the Japanese Ministry of International Trade and Industry (MITI, 1976). This ready biodegradability test was carried out according to the OECD Guideline TG 301C: MITI (I), using activated sludge as inoculum at a concentration of 30 ppm. The test concentration used in the study was 100 ppm. After 14 days (end of the study), the test substance reached a biodegradation value of 1.1%. Thus, these results support those from the key study and Amides, C16-C18 (even), N,N'-ethylenebis can be considered as not readily biodegradable but the substance is hardly soluble in water and the very low solubility of the test substance may reduce the availability of the substance in the test medium.
Therefore, an additional calculation was used to investigate the biodegradation of Amides, C16-C18 (even), N,N'-ethylenebis. A QSAR prediction using the following models Biowin1, Biowin2, Biowin3, Biowin4, Biowin5, Biowin6 and Biowin7 shows that the substance is not persistent in the environment and the biodegradation takes place in a time frame of weeks.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
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