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EC number: 231-912-9 | CAS number: 7778-74-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 03, 2017 - December 13, 2017
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- adopted July 22, 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- dated May 30, 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- ISO 8192:2007
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the municipal sewage treatment plant Bensheim, Germany.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +- 2 °C
- pH:
- 7 - 8
- Dissolved oxygen:
- 6.6 - 8.7 [mg O2/L]^2
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 383.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of respiration due to nitrification
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 14.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Total respiration:
In comparison to the inoculum controls the total respiration rate of the activated sludge was moderately inhibited at the three lower test concentrations (up to 100 mg/L) and strongly inhibited for test item concentrations of 320 mg/L and 1000 mg/L. The inhibition was between 18% and 28% at test concentrations of 10 mg/L, 32 mg/L and 100 mg/L. For the test item concentrations of 320 mg/L and 1000 mg/L the inhibition rates were 39.2% and 47.4%, respectively. Concentrations exceeding 1000 mg/L nominal were not tested. No clear concentration dependant relationship could be determined.
No clear concentration dependant relationship could be determined. Due to the higher inhibition at 10 mg test item/L than 32 mg test item/L, only the EC20 could be determined.
The 3-hour EC20 for total respiration was established to be 14.8 mg/L; the NOEC was determined to be lower 10 mg/L, as the inhibition in the lowest test item concentration of 10 mg/L was already 24.9%.
Potassium perchlorate had effects on the respiration (total respiration and nitrification respiration) of activated sludge microorganisms from 10 mg/L up to 1000 mg/L; doserelated effects could not be clearly observed over all test item concentrations. Therefore, the NOEC for total respiration, heterotroph respiration and nitrification respiration is below a test item concentration of 10 mg/L. Contrariwise, the inhibition of total respiration and heterotrophic respiration by Potassium perchlorate never reached 50%, suggesting that the EC50 values are above the highest tested concentration of 1000 mg/L. - Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-dichlorophenol for the total respiration was 4.0 mg/L and therefore in the range of 2 to 25 mg/L for the used activated sludge batch.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Total respiration: The 3-hour EC20 for total respiration was established to be 14.8 mg/L; the NOEC was determined to be lower 10 mg/L, as the inhibition in the lowest test item concentration of 10 mg/L was already 24.9%.
Respiration without nitrification (Heterotrophic Respiration): The 3-hour EC20 for heterotrophic respiration was determined to be 13.7 mg/L; the NOEC was determined to be <10 mg/L.
Respiration based on nitrification: For the nitrification respiration, the 3-hour EC20 and EC50 were established to be 12.9 mg/L and 383.5 mg/L, respectively; the NOEC was determined to be <10 mg/L. - Executive summary:
The influence of the test item potassium perchlorate on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item after an incubation period of 3 hours.
Reference
Description of key information
Total respiration: The 3-hour EC20 for total respiration was established to be 14.8 mg/L; the NOEC was determined to be lower 10 mg/L, as the inhibition in the lowest test item concentration of 10 mg/L was already 24.9%.
Respiration without nitrification (Heterotrophic Respiration): The 3-hour EC20 for heterotrophic respiration was determined to be 13.7 mg/L; the NOEC was determined to be <10 mg/L.
Respiration based on nitrification: For the nitrification respiration, the 3-hour EC20 and EC50 were established to be 12.9 mg/L and 383.5 mg/L, respectively; the NOEC was determined to be <10 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 383.5 mg/L
Additional information
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