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Diss Factsheets
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EC number: 700-881-8 | CAS number: 83145-78-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014-01-15 to 2014-01-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and guideline compliant study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- potassium bisoxalatoborate KBOB
- IUPAC Name:
- potassium bisoxalatoborate KBOB
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Stored at room temperature, protected from light.
Constituent 1
Test animals / tissue source
- Species:
- other: cow
- Strain:
- not specified
Test system
- Vehicle:
- physiological saline
- Controls:
- other: not applicable; but in vitro controls
- Amount / concentration applied:
- 20 % in physiological saline (0.9 % NaCl).
- Duration of treatment / exposure:
- 4 hours ± 5 minutes
- Observation period (in vivo):
- 90 minutes
- Number of animals or in vitro replicates:
- not applicable
- Details on study design:
- The assay uses isolated corneas from animals freshly slaughtered at an abattoir. Fresh eyes were collected from the abattoir on the test day and transported in HBSS containing Pen/Strep on ice to the laboratory, where cornea preparation was initiated immediately. The eyes were carefully examined for defects and any defective eyes discarded. The tissue surrounding the eyeball was carefully pulled away and the cornea excised, leaving 2-3 mm of sclera. The isolated corneas were stored in a petri dish containing HBSS and again examined for defects before use in the test.
Negative control: physiological saline (0.9 % NaCl)
Positive control: imidazole 20 % in physiological saline (0.9 % NaCl)
Results and discussion
In vitro
Results
- Irritation parameter:
- in vitro irritation score
- Value:
- 102.43
In vivo
- Irritant / corrosive response data:
- As the mean in vitro irritation score for the test item was determined to be > 55 (102.43), the test item is identified as inducing serious eye damage.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye)
- Remarks:
- Criteria used for interpretation of results: other: UN GHS
- Conclusions:
- Based on the in vitro eye corrosion/irritation study with bovine corneas, KBOB is considered a severe eye irritant.
- Executive summary:
They eye corrosion potential of KBOB was investigated in the in vitro bovine corneal opacity and permeability assay according to OECD Technical Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants).
The test item was suspended in physiological saline (0.9 %) as a concentration of 20 %. Isolated bovine corneas were mounted in corneal holders, and after an initial incubation in RPMI medium (in both anterior and posterior chamber), their opacity was measured with an opacitometer. Three corneas were used as negative controls, positive controls and for the test item, each. 750 μL of the test item or the control substance was introduced into the anterior chamber, and after 4 hours ± 5 minutes of incubation at 32 ± 1 °C, the substances were removed and the epithelium washed with MEM media containing phenol red until free of test substance, then with RPMI, then an opacity measurement was performed. After a 90-minute incubation (32 ± 1 °C) in fresh RPMI with 1 mL of a 5 mg/mL sodium fluorescein solution added to the anterior chamber, the medium from the posterior chamber was removed and its optical density (OD490) at 490 nm was determined, using a spectrophotometer. The initial opacity reading of each cornea was subtracted from the final opacity reading to obtain the change in opacity, corrected by subtracting the average change in opacity observed for the negative controls, and a mean opacity value was calculated for each treatment. The OD490 values were similarly corrected by subtracting a mean blank-well OD490 from each OD490 for a test article, then the average corrected negative-control OD490 from each test-item or positive-control OD490, and a mean OD490 was then calculated for each treatment. The in vitro irritation score (IVIS) was then calculated as the mean opacity value – (15 x mean OC490 value).
The resulting mean in vitro irritation score for the test item was 102.43. According to the evaluation criteria, KBOB is classified into UN GHS Category 1.
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