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EC number: 279-349-8 | CAS number: 79916-07-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals
- Author:
- Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E.
- Year:
- 1 986
- Bibliographic source:
- Environmental Mutagenesis; Volume 8, Issue Supplement S7, pages 1–55, 1986
- Reference Type:
- publication
- Title:
- Study ID: 840758
- Author:
- NTP
- Year:
- 2 012
- Bibliographic source:
- NTP (National Toxicological Program)by Agency for Toxic Substances and Disease Registry; Division of Toxicology/Toxicology Information Branch, 1600 Clifton Road NE, E-29, Atlanta, Georgia 30333
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: refer below principle
- Principles of method if other than guideline:
- The Salmonella Ames Test: Preincubation method was performed to determine the mutagenic nature of the test compound N,N-dimethylaniline
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- N,N-dimethylaniline
- EC Number:
- 204-493-5
- EC Name:
- N,N-dimethylaniline
- Cas Number:
- 121-69-7
- Molecular formula:
- C8H11N
- IUPAC Name:
- N,N-dimethylaniline
- Details on test material:
- - Name of test material: N,N-dimethylaniline
- Molecular formula: C8H11N
- Molecular weight: 121.182 g/mol
- Substance type: Organic
- Physical state: liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: N,N-dimethylaniline
- Molecular formula: C8H11N
- Molecular weight: 121.182 g/mol
- Substance type: Organic
- Physical state: liquid
Method
- Target gene:
- histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA1535, TA1537, TA98, and TA100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- Not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- 10% RLI = induced male Sprague Dawley rat liver S9,10% HLI = induced male Syrian hamster liver S9
- Test concentrations with justification for top dose:
- 0, 3, 10, 33, 100, 333, 1000 µg/Plate
- Vehicle / solvent:
- Dimethyl Sulfoxide
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl Sulfoxide
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- in the absence of S9 activation
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- For TA98
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl Sulfoxide
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- in the absence of S9 activation
- Positive control substance:
- sodium azide
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethyl Sulfoxide
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- in the presence of S9 activation by 10% HLI/RLI
- Positive control substance:
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation method
DURATION
- Exposure duration: 48 hour - Rationale for test conditions:
- Not specified
- Evaluation criteria:
- An increase in the nuber of revertants/plate was noted.
- Statistics:
- Yes standard deviation was observed.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA1535, TA1537, TA98, and TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: The test chemical was initially tested with strain TA100 in the presence and the absence of the metabolic activation systems, over a wide dose range with an upper limit of 10 mg/plate, or less when solubility problems were encountered.
Any other information on results incl. tables
Strain: TA100
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
85 |
2.7 |
93 |
3.7 |
123 |
10 |
87 |
3.1 |
97 |
2.2 |
94 |
9 |
3 |
107 |
3.2 |
107 |
8.5 |
|
|
104 |
4.7 |
|
|
108 |
11.7 |
10 |
89 |
5.6 |
102 |
8 |
112 |
8.7 |
89 |
6 |
110 |
7.9 |
108 |
4.8 |
33 |
104 |
11.3 |
97 |
10.3 |
113 |
1.8 |
104 |
3 |
110 |
6.2 |
101 |
6.2 |
100 |
109 |
12.5 |
95 |
9.9 |
102 |
9.3 |
87 |
3.3 |
113 |
4.5 |
105 |
2.6 |
333 |
102 |
3.5 |
81 |
3.7 |
122 |
12.7 |
93 |
2.7 |
119 |
11.1 |
102 |
8.1 |
1000 |
|
|
|
|
40s |
9.3 |
|
|
78s |
13.4 |
|
|
Positive Control |
382 |
7.7 |
345 |
17.7 |
1336 |
56.6 |
1132 |
40.3 |
461 |
26.6 |
441 |
9.1 |
Strain: TA1535
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
23 |
1.9 |
28 |
3.7 |
9 |
1.9 |
6 |
0.3 |
8 |
0.6 |
10 |
1.2 |
3 |
14 |
0.9 |
20 |
3.8 |
|
|
7 |
1 |
|
|
9 |
0.3 |
10 |
14 |
3.2 |
21 |
2.2 |
10 |
1.2 |
7 |
2.8 |
7 |
0.7 |
8 |
2.2 |
33 |
16 |
1.5 |
18 |
1.9 |
8 |
0.3 |
4 |
1.2 |
6 |
0 |
7 |
0.7 |
100 |
15 |
0.3 |
18 |
2.8 |
6 |
0.3 |
7 |
0.6 |
7 |
0.9 |
6 |
1 |
333 |
15 |
2.1 |
20 |
2.9 |
7 |
1.2 |
6 |
1 |
6 |
1 |
5 |
0.9 |
1000 |
|
|
|
|
2s |
1 |
|
|
3s |
1.2 |
|
|
Positive Control |
519 |
6.8 |
324 |
21.2 |
438 |
43 |
452 |
9.8 |
158 |
11.5 |
187 |
9.9 |
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; T = Toxic; c = Contamination
Strain: TA1537
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
7 |
0.9 |
7 |
2.7 |
8 |
2.3 |
6 |
1.7 |
6 |
1.9 |
3 |
0.9 |
3 |
3 |
0.3 |
5 |
1.8 |
|
|
9 |
1.8 |
|
|
7 |
0.6 |
10 |
4 |
1.5 |
5 |
0 |
3 |
0.9 |
4 |
1.2 |
5 |
0.7 |
7 |
0 |
33 |
4 |
0.3 |
4 |
0.9 |
5 |
0.6 |
5 |
0.7 |
3 |
0.6 |
4 |
0.9 |
100 |
6 |
2.2 |
3 |
0 |
7 |
0.3 |
7 |
0.9 |
5 |
1.2 |
3 |
0.7 |
333 |
2 |
1.5 |
5 |
1.7 |
5 |
0.6 |
6 |
2.1 |
5 |
0.9 |
4 |
0 |
1000 |
|
|
|
|
2s |
0.9 |
|
|
7s |
1.2 |
|
|
Positive Control |
556 |
5.2 |
154 |
23.7 |
298 |
10.1 |
339 |
18.3 |
128 |
3.9 |
105 |
6.2 |
Strain: TA98
Dose |
No Activation |
No Activation |
10% HLI |
10% HLI |
10% RLI |
10% RLI |
||||||
Protocol |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
Preincubation |
||||||
ug/Plate |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
Mean |
± SEM |
0 |
14 |
0.3 |
15 |
1.9 |
27 |
1.5 |
30 |
1.2 |
17 |
1.8 |
25 |
3.8 |
3 |
18 |
0.7 |
15 |
4.5 |
|
|
27 |
6.6 |
|
|
25 |
0.6 |
10 |
14 |
0.7 |
12 |
2.2 |
23 |
5 |
29 |
2.1 |
19 |
3.5 |
24 |
2.6 |
33 |
11 |
2.7 |
8 |
0.3 |
25 |
0.9 |
23 |
4 |
22 |
2.8 |
22 |
3.9 |
100 |
10 |
1.5 |
8 |
0.9 |
25 |
2.4 |
24 |
3 |
22 |
2 |
22 |
2.8 |
333 |
9 |
1.8 |
12 |
2.5 |
26 |
3.4 |
22 |
2.5 |
22 |
1.9 |
20 |
0.7 |
1000 |
|
|
|
|
4s |
0.3 |
|
|
8s |
4.6 |
|
|
Positive Control |
1034 |
21.2 |
354 |
30.5 |
1104 |
32.4 |
1444 |
62.5 |
402 |
17 |
404 |
29.7 |
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; T = Toxic; c = Contamination
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
Applicant's summary and conclusion
- Conclusions:
- N,N-dimethylaniline indicated negative result for genetic toxicity in Ames test conducted on to S. typhimurium TA98, TA100,TA37 and TA1535 with and without metabolic activation by 10% HLI/RLI S9 system.
- Executive summary:
Gene mutation toxicity study was performed to evaluate the mutagenic nature of the test compound N,N-diethylaniline. Salmonella Ames assay was performed by the preincubation method using the Salmonella typhimurium strains TA98, TA100,TA37 and TA1535 with and without S9 metabolic activation system. The test compound gave negative result for genetic toxicity in Ames test conducted on to S. typhimurium TA98, TA100,TA37 and TA153 with and without metabolic activation by 10% HLI/RLI S9 system. Hence, N,N-diethylaniline (RA CAS no 121 -69 -7) is not likely to classify as gene mutant in vitro.
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