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EC number: 947-285-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short-term toxicity to aquatic invertebrates:
EC50 (Daphnia magna, 48 h) = 48 mg/L (geom. mean of measured conc.)
Toxicity to aquatic plants other than algae:
ErC50 (Lemna minor, 7d) = 141.40 mg/L (geom. mean of measured conc.)
Microorganisms:
IC 50 (3 h) > 100 mg/L
Additional information
Invertebrate acute toxicity
A static test on the analogue substance was performed according to the OECD Guideline 202 (2004) and the EU method C.2 of the Regulation EC 440/2008. 5 concentrations were used ranging from 4.6 to 100 mg/L (nominal concentration). For each test concentration and the blank control, 20 Daphnia were exposed to the test item for 48 hours. After 24 and 48 hours, the immobilised Daphnia were counted.
Potassium dichromate K2Cr2O7was used as positive controlin a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using photometer-determination. The concentrations determined at the start of the test were between 45 and 81 % of the nominal concentration. At the end of the test the determined concentrations were between 94 and 108 % of the nominal concentration. Therefore, the determination of the biological results was based on geometric mean of measured concentrations.
All validity criteria were met.
Aquatic Plants toxicity
A study was performed on the analogue substance in order to evaluate the toxicity of tehe test item towards Lemna minor following the OECD Guideline 221 (2006).The study was performed using 5 concentrations ranging from 1.0 to 100 mg/L (nominal concentration). Incubation time was 7 days. The frond number of each replicate was determined at the beginning, at day 2 and 5 during the test and at the end of the experiment. Additionally, the dry mass of 12 representative fronds was determined at the beginning of the experiment. At the end of the experiment the dry mass of each replicate was determined.
Growth rate µ and the yield were determined from the frond number and the drymass at the respective observation times.
At the start and at the end of the test, the content of the test item in the test solutions was determined using a Photometer.
The measured concentrations lay between 27 % and 49 % of the nominal concentrations at the beginning of the test and between 23 % and 61 % of the nominal concentrations at the end of the test. At the end of the test the measured concentration of treatment 1 mg/L was lower than the LOQ. Therefore, the determination of the results was based on the geometric mean of the measured. Half the LOQ (0.05 mg/L) of the 7d value was used to calculate the geometric mean of the 1 mg/L treatment.
The measured concentrations were much lower than the nominal concentration. It is very likely that the solubility in STEINBERG medium is lower than the solubility in demineralized water. Due to a strong colour of the stock solution at the concentration 100 mg/L, presence of undissolved test item could not be observed. This can be stated as uncritical for the outcome of the study because in the lower concentrations no undissolved test item was present and also toxicity was observed and the biological results were based on the real measured concentration.
The 7d-EC50s of 3,5-Dichlorophenol (1,3-Dichloro-5-hydroxybenzene, C6H4Cl2O, CAS-No. 591-35-5) were determined in a separate reference test. The EC50value of the growth rate lay within the desired range of 1.7 - 5.7 mg/L, mentioned in the paper “OECD Lemna Growth Inhibition Test, Development and ring testing of draft OECD test guideline”, Research and Development Technical Report EMA 003, page 34.
Additional tests
The Substance was tested for respiration inhibition test on aerobic waste-water bacteria following the OECD Guideline 209 (1984). The validity criteria were fullfilled.
The IC 50 (3 h) was determined to be > 100 mg/L.
Moreover, an old test on the substance is available: it was performed in accordance with internal procedures of the testing facility (modifizierte routine bioassay method vom 1.11.1974). Unfortunately, the reliability of the data cannot be judged because of the lacking of details about test material and testing procedures and conditions. Data is here reported only for completeness purpose.
LC0 (48 h, Salmo gairdnerii) = 20 mg/L
LC50 (48 h, Salmo gairdnerii) = 10 mg/L
LC100 (48 h, Salmo gairdnerii) = 4 mg/L
REFERENCE: Details in attachemnt.
JUSTIFICATION FOR CLASSIFICATION OR NON-CLASSIFICATION
According to the CLP Regulation (EC) No. 1272/2008, Part 4: Environmental Hazards, substances can be classified as hazardous to the aquatic environment when the following criteria are met:
A) Acute (short-term) aquatic hazard Category Acute 1: 96-hour LC50 (fish) and/or 48-hour EC50 (crustacea) and/or 72- or 96-hour ErC50 (algae or other aquatic plants) ≤ 1 mg/l.
B) Long-term aquatic hazard (iii) Substances for which adequate chronic toxicity data are not available and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).
- Category Chronic 1: 96-hour LC50 (fish) and/or 48-hour EC50 (crustacea) and/or 72- or 96-hour ErC50 (algae or other aquatic plants) ≤ 1 mg/l;
- Category Chronic 2: 96-hour LC50 (fish) and/or 48-hour EC50 (crustacea) and/or 72- or 96-hour ErC50 (algae or other aquatic plants)> 1 to ≤10 mg/l;
- Category Chronic 3: 96-hour LC50 (fish) and/or 48-hour EC50 (crustacea) and/or 72- or 96-hour ErC50 (algae or other aquatic plants) > 10 to ≤ 100 mg/l.
The substance is not rapidly degradable and the acute short-term test to invertebrates fixed effect levels which meets the criterion for the hazard Category Chronic 3 according to the CLP Regulation (EC) No. 1272/2008.
Based on the read across considerations same results and classification apply to Acid Brown 360:1.
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