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EC number: 422-030-7 | CAS number: 91538-82-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24.-28.04.2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- EEC Directive 92/69
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: reconstituted water
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Species: zebra fish (Danio rerio), originated West-Aquarium
Breeder: in house
Size: 2.0 + 1.0 cm
Body weight per group: 6.0 and 8.1 g
The species is internationally accepted for this type of study. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 267 mg CaCO3/L
- Test temperature:
- 24 to 26 °C
- pH:
- 7.46 - 7.99
- Dissolved oxygen:
- > 60 % saturation
- Nominal and measured concentrations:
- 100 mg/L
- Reference substance (positive control):
- not required
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: >0.0114 mg/L (limit of water solubility)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: >0.0114 mg/L (limit of water solubility)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: >0.0114 mg/L (limit of water solubility)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: >0.0114 mg/L (limit of water solubility)
- Details on results:
- No signs of toxicity have been observed throughout the study up to the limit of water solubility (0.0114 mg/L).
- Sublethal observations / clinical signs:
After exposure of zebra fish to a saturated aqueous solution of the test item, with a nominal concentration of 100 mg/L for 96 hours, the following results were obtained:
24h 48 h 72h 96h 0/10 0/10 0/10 0/10 Zebra fish exposed to an aqueous solution, with a nominal concentration of 100 mg test item per liter, were not affected. The analysis revealed, that the solubility the test item in water was < 0.0114 mg/L. Because of this low water solubility and the fact that the study was performed as a limit test, the test material concentrations in the aqueous medium at the start and the end of this study could not be quantified.
For the test material the following LC50values and no effect concentration (EC0) for zebra fish were determined.
24h LC50 > 0.0114 (nominal> 100 mg/L)
48 h LC50 > 0.0114 (nominal> 100 mg/L)
72 h LC50 > 0.0114 (nominal> 100 mg/L)
96 h LC50 > 0.0114 (nominal> 100 mg/L)
EC0 > 0.0114 (nominal> 100 mg/L)
Control
Time
3 h
24 h
48 h
72 h
96 h
Observed fish
10
10
10
10
10
Symptom
No abnormalities detected
10
10
10
10
10
Test material group
Time
3 h
24 h
48 h
72 h
96 h
Observed fish
10
10
10
10
10
Symptom
No abnormalities detected
10
10
10
10
10
In the control and the test material group no clinical findings were observed during the
experimental part.
Control
Time
3 h
24 h
48 h
72 h
96 h
Observed fish
10
10
10
10
10
Symptom
No abnormalities detected
10
10
10
10
10
Test material group
Time
3 h
24 h
48 h
72 h
96 h
Observed fish
10
10
10
10
10
Symptom
No abnormalities detected
10
10
10
10
10
In the control and the test material group no clinical findings were observed during the
experimental part.
Mortality
Concentration
24 h
48 h
72 h
96 h
Control
0/10
0/10
0/10
0/10
100
0/10
0/10
0/10
0/10
All fish survived the observation period.
LC50
24 h
48 h
72 h
96 h
Nominal
>100 mg/L
>100 mg/L
>100 mg/L
>100 mg/L
Analytical
>0.0114 mg/L
>0.0114 mg/L
>0.0114 mg/L
>0.0114 mg/L
A dose group with nominal 100 mg/L test item (maximal solubility <0.0114 mg/L analytically) revealed no aquatic toxicity in this test system.The analytical 24, 48, 72,and 96 h LC50value exceed the maximal solubility of the test material. No remarkable observations were made concerning the appearance of the solution of the test material preparation. The test medium was a clear preparation and stayed unchanged throughout the study.
- Validity criteria fulfilled:
- yes
- Conclusions:
- An aqueous solution of the test material of a nominal concentration of 100 mg/L revealed no aquatic toxicity in the test system. The 96 h LC50 value to zebra fish exceeded the maximal solubility (< 0.0114 mg/I, analytically observed) of the test material in reconstituted water and could thus not be determined.
- Executive summary:
Study design
The objective of this aquatic toxicity study in zebra fish (Danio rerio) was to provide information on environmental hazards likely to arise from exposure of the test material and to serve as a basis for classification and labeling purposes. For this purpose, 10 fish (test material group) were exposed over 96 hours, under defined conditions in a limit test. A further 10 fish were used as a control group. The fish were observed for signs of toxicity or death for 96 hours. Zebra fish of the test material group were exposed to a saturated aqueous test material solution of nominal 100 mg/L (limit test) in an open static system. The study was conducted according to Good Laboratory Practice (GLP) and followed the OECD Guidelines for the Testing of Chemicals, No. 203 Fish Acute Toxicity Test.
Results
After exposure of zebra fish to a saturated aqueous solution of test material with a nominal concentration of nominal 100 mg/L for 96 hours, the following results were obtained:
Mortality 24h 48h 72h 96 h Control Group 0/10 0/10 0/10 0/10 Test material group 0/10 0/10 0/10 0/10
Zebra fish exposed to an aqueous solution, with a nominal concentration of 100 mg test item per liter, were not affected.
The analysis revealed, that the solubility of the test material in water was < 0.0114 mg/L. Because of this low water solubility and the fact that the study was performed as a limit test, the test material concentrations in the aqueous medium at the start and the end of this study could not be quantified.
For the test material, the following LC50 values for zebra fish were determined.
24 h LC50 > 0.0114 mg/L (nominal > 100 mg/L)
48 h LC50 > 0.0114 mg/L (nominal > 100 mg/L)
72 h LC50 > 0.0114 mg/L (nominal > 100 mg/L)
96 h LC50 > 0.0114 mg/L (nominal > 100 mg/L)
Conclusion
An aqueous solution of the test material of a nominal concentration of 100 rng/L revealed no aquatic toxicity in the test system. The 96 h LC50 value to zebra fish exceeded the maximal solubility (< 0.0114 mg/I, analytically observed) of the test material in reconstituted water and could thus not be determined.
Reference
Description of key information
An aqueous solution of the test material of a nominal concentration of 100 rng/L revealed no aquatic toxicity in the test system. The 96 h LC50 value to zebra fish exceeded the maximal solubility (< 0.0114 mg/I, analytically observed) of the test material in reconstituted water and could thus not be determined.
Key value for chemical safety assessment
Additional information
The study was conducted according to Good Laboratory Practice (GLP) and followed the OECD Guidelines for the Testing of Chemicals, No. 203 Fish Acute Toxicity Test. Zebra fish exposed to an aqueous solution, with a nominal concentration of 100 mg test item per liter, were not affected. The analysis revealed, that the solubility of the test material in water was < 0.0114 mg/L. The 96 h LC50 value to zebra fish exceeded the maximal solubility (< 0.0114 mg/I, analytically observed) of the test material in reconstituted water and could thus not be determined.
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