Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 276-127-2 | CAS number: 71873-51-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September from 01st to 05th, and from 20th to 26th, 1997 (range finding and main test, respectively).
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 17 July, 1992
- GLP compliance:
- no
- Analytical monitoring:
- no
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: guppy
- Length at study initiation: 1-3 cm
BREEDING
- Vessels: parental and young fish population was held in two 250 l glass aquaria.
- Illumination: 14 hours light.
- Temperature: 23 ± 2 °C
- Medium: continuously aerated and filtered tap water from Schlieren. The bottom of the aquaria was covered with gravel (<1.5 cm). Every month, one third of the total volume of the aquaria was replaced by fresh tap water.
- Feed: sera micropan, Sera vipan (Sera apanristic GmbH, D-52525 Heinsberg, Germany) daily. The fish were last fed 24 h before the start of the definitive test.
- Health check: during 7 days before the definitive test, mortality in the batch of which fish were used for the test was less than 5 %. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21 ± 1 °C
- pH:
- Vessels: 7.7 - 8.5
Control: 7.7 - 8.4 - Dissolved oxygen:
- Vessels: 6.8 - 8.3 mg/l
Control: 7.7 - 8.0 mg/l - Nominal and measured concentrations:
- 0.125, 0.250, 0.500, 1.00 and 2.00 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 l aquaria, all-glass.
- No. of organisms per vessel: 7 fish per test concentration.
- No. of vessels per concentration: 1 replicate.
- No. of vessels per control: 1 replicate.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water f rom Zürich-Schlieren, aerated.
OTHER TEST CONDITIONS
- Photoperiod: 14 h photoperiod a day, supplied by overhead white fluorescent tubes.
MEASUREMENTS
- Oxygen, pH, temperature: prior to the addition of fish to the test medium and after 2, 24, 48, 72 and 96 h of exposure
- Observations of the fish: made after 2, 24, 48, 72 and 96 h of exposure.
- Mortality: mortality of the fish was recorded at the same time intervals. Dead fish were removed immediately from the test medium and weight and length recorded.
RANGE FINDING TEST
- Concentration: 1, 10 and 100 mg/l
- No. of organisms per vessel: 3 fish per concentration.
- Results: 100 % mortality was observed at 100 and 10 mg/l after 2 h and at 1 mg/l after 24 h of exposure, respectively. No mortality was observed in the control after 96 h of exposure. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.707 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- ln the definitive test all fish died at the nominal concentrations 2.00 and 1.00 mg/l after 24 hours of exposure.
No mortality was observed at 0.500, 0.250 and 0.125 mg/l, respectively and in the control after 96 hours of exposure.
Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observed.
The no-observed-effect concentration (NOEC) was 0.500 mg/l.
100 % mortality was seen at the nominal concentration of 1.00 mg/l.
According to the physical data of the test substance provided by the supplier (negligible vapour pressure, sufficient stability and solubility in water) it was assumed that the nominal concentration has been satisfactorily maintained during the whole test procedure. Therefore, no analytical determination was conducted. - Sublethal observations / clinical signs:
Incidence of mortality observed.
Concentration Number of dead fish observed at different points in time after start of exposure 2 h 24 h 48 h 72 h 96 h Control 0 0 0 0 0 0.125 0 0 0 0 0 0.250 0 0 0 0 0 0.500 0 0 0 0 0 1.00 1 6 - - - 2.00 6 1 - - - - Conclusions:
- LC50 (96h): 0.707 mg/l (estimated on the basis of nominal conc.)
- Executive summary:
The acute lethal toxicity of test item to Poecilia reticulata (Guppy) was investigated under static exposure conditions over a period of 96 h. 7 fish per concentration were exposed to the test substance.
The nominal concentrations as selected by a range finding test were 0.125, 0.250, 0.500, 1.00 and 2.00 mg/l, respectively. The individual test concentrations were prepared by dilution of a stock solution. No chemical analyses of the test concentrations were conducted.
In the definitive test all fish died at the nominal concentrations 2.00 and 1.00 mg/l after 24 h of exposure. No mortality was observed at 0.500, 0.250 and 0.125 mg/l, respectively, and in the control after 96 h of exposure. Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observed.
Based on the data, the nominal lethal concentration of test item to Poecilia reticulata was calculated to be 0.707 mg/l. The no-observed-effect concentration (NOEC) was 0.500 mg/l. 100 % mortality was seen at the nominal concentration of 1.00 mg/l.
Conclusion
LC50 (96h): 0.707 mg/l (estimated on the basis of nominal conc.)
Reference
Description of key information
LC50 (96h): 0.707 mg/l (estimated on the basis of nominal conc.)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.707 mg/L
Additional information
The acute lethal toxicity of test item was investigated under static exposure conditions over a period of 96 h, using Poecilia reticulata.
The nominal concentrations were selected by a range finding test and were 0.125, 0.250, 0.500, 1.00 and 2.00 mg/l. The individual test concentrations were prepared by dilution of a stock solution. No chemical analyses of the test concentrations were conducted. In the definitive test, all fish died at the nominal concentrations 2.00 and 1.00 mg/l after 24 h of exposure. No mortality was observed at 0.500, 0.250 and 0.125 mg/l, respectively, and in the control after 96 h of exposure. Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observed. Therefore, the LC50 (96h) resulted to be between 0.50 and 1.0 mg/l; the value was estimated to be 0.707 mg/l.
A second experiment conducted on Oncorhynchus mykjss, is available. A range-finding experiment was conducted at the nominal concentrations of 0.01, 0.10, 1.0, 10 and 100 mg/l; for the main test, 0.10 mg/l was selected as limit nominal concentration. 1 deaths was recorded in both the main experiment replicates, therefore the LC50 (96h) was indicated to be higher than 0.10 mg/l (nominal).
The outcomes for the two experiments are consistent: the LC50 (96h) value resulted to be between 1.0 and 0.5 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.