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EC number: 214-874-8 | CAS number: 1204-28-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-04-30 to 1991-07-10
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study performed according to appropriate guideline and GLP, unable to confirm conformity of replicate control respiration rates.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Trimetillic anhydride
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 100 - <= 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The 3-hour EC50 obtained from the test was between 100 and 500 mg/L.
- Results with reference substance (positive control):
- The positive control, 3,5-dichlorophenol, exhibited an EC50 of 10.2 mg/L in the range-finding test and 4.6 mg/L in the definitive test.
- Validity criteria fulfilled:
- yes
- Remarks:
- The EC50 of the reference inhibitor 3,5-DCP was 10 mg/L and 5 mg/L (rounded values) in the range-finder and definitive tests, respectively. No information presented in the report about the conformity of respiration rates in replicate control vessels.
- Conclusions:
- The respiration of activated sludge was unaffected by 3-h exposure to trimellitic anhydride at nominal concentrations of up to 100 mg/L, but profound inhibition occurred at concentrations equal to or greater than 500 mg trimellitic anhydride/L. Based on the combined observations of a range-finder and definitive test, and nominal concentrations, the 3-hour EC50 was >100, <500 mg trimellitic anhydride/L.
- Executive summary:
The toxicity of trimellitic anhydride (source substance) was determined in an activated sludge respiration inhibition test according to OECD guideline 209. Activated sludge obtained from a predominantly domestic sewage plant was exposed over a period of three hours to trimellitic anhydride at nominal concentrations of 1, 10 and 100 mg/L in a preliminary range-finder and at 500, 1000, 2000 and 4000 mg/L in the subsequent definitive test. Inhibition, relative to the control rate, was <10% in all the range-finder treatments, but >/=95% in all the definitive test treatments. The 3 -h EC50 could not be calculated reliably, but it lies in the range >100, <500 mg trimellitic anhydride/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- The justification of the read across is described in the document attached below.
- Reason / purpose for cross-reference:
- read-across source
- Specific details on test material used for the study:
- 4-chloroformylphthalic anhydride
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100 - 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The respiration of activated sludge was unaffected by 3-h exposure to trimellitic anhydride (source substance) at nominal concentrations of up to 100 mg/L, but profound inhibition occurred at concentrations equal to or greater than 500 mg trimellitic anhydride/L. Based on the combined observations of a range-finder and definitive test, and nominal concentrations, the 3-hour EC50 was between 100 and 500 mg/L.
- Executive summary:
The toxicity of trimellitic anhydride (source substance) was determined in an activated sludge respiration inhibition test according to OECD guideline 209. Activated sludge obtained from a predominantly domestic sewage plant was exposed over a period of three hours to trimellitic anhydride (source substance) at nominal concentrations of 1, 10 and 100 mg/L in a preliminary range-finder and at 500, 1000, 2000 and 4000 mg/L in the subsequent definitive test. Inhibition, relative to the control rate, was <10% in all the range-finder treatments, but >/=95% in all the definitive test treatments. The 3-h EC50 could not be calculated reliably, but it lies in the range >100, <500 mg/L.
Based on the close structural similarity of trimellitic anhydride (source substance) and trimellitic anhydride mono-chloride (target substance), these results can be taken into account for the target substance.
Referenceopen allclose all
Table 1: Effects on the respiration rate of activated sludge following 3 -h exposure to trimellitic anhydride (Range-finder data)
Trimellitic anhydride nominal concentration (mg/L) |
Respiration rate, mg O2/L*h |
Respiration inhibition (%) relative to control |
0.0 (control) |
97.8 |
- |
1 |
107.28 |
stimulation |
10 |
97.2 |
0.6 |
100 |
91.5 |
6 |
7.5 mg 3,5-DCP/L (reference inhibitor) |
62.58 |
36 |
15.0 mg 3,5-DCP/L (reference inhibitor) |
30.9 |
68 |
30.0 mg 3,5-DCP/L (reference inhibitor) |
13.92 |
86 |
Table 2: Effects on the respiration rate of activated sludge following 3 -h exposure to trimellitic anhydride (Definitive test data)
Trimellitic anhydride nominal concentration (mg/L) |
Respiration rate, mg O2/L*h |
Respiration inhibition (%) relative to control |
0.0 |
153.6 |
- |
500 |
8.16 |
95 |
1000 |
1.32 |
99 |
2000 |
0.78 |
99.5 |
4000 |
0.78 |
99.5 |
7.5 mg 3,5-DCP/L (reference inhibitor) |
51.6 |
66 |
15.0 mg 3,5-DCP/L (reference inhibitor) |
10.2 |
93 |
30.0 mg 3,5-DCP/L (reference inhibitor) |
4.5 |
97 |
Description of key information
Read-across, OECD guideline 209, GLP, key study, validity 2:
Toxicity to microorganisms: 100 mg/L< EC50 < 500 mg/L; NOEC = 100 mg/L based on nominal concentrations.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
In a reliable study of the toxicity of trimellitic anhydride to aquatic microorganisms (Lebertz, 1991b), the 3 hour EC50, based on inhibition of respiration of activated sludge, was between 100 and 500 mg trimellitic anhydride-equiv/L. Inhibition, relative to the untreated control, was observed at concentrations ≥ 500 mg/L and the NOEC was 100 mg/L.
Rapid hydrolysis of the anhydride to trimellitic acid is presumed to have occurred under the conditions of the test and it is likely that salts were formed by interaction between trimellitic acid and the mineral constituents of the synthetic sewage present in the test system.
No pH measurements were reported; consequently it is uncertain to what degree a lowering of pH may have caused or contributed to the observed effect.
Based on the close structural similarity of trimellitic anhydride (source substance) and trimellitic anhydride mono-chloride (target substance), these results can be taken into account for the target substance.
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