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EC number: 700-526-7 | CAS number: 1333488-95-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (1984)
- GLP compliance:
- yes
Test material
- Reference substance name:
- methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate
- EC Number:
- 700-526-7
- Cas Number:
- 1333488-95-1
- Molecular formula:
- C12H7F2NO4
- IUPAC Name:
- methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Composite samples (about 150 ml per concentration and control) of each test concentration were drawn immediately before exposure from appropriate dilution of the stock solution and after 72 hours exposure by mixing identical volumes of the test solutions taken from the approximate Center of the test vessels and kept at - 18°C to - 22°C until analysis.
Test solutions
- Details on test solutions:
- Stock solutions:
100 mg test substance were mixed with and made up to 1000 ml with test water.
Test concentrations:
Calculated amounts of the stock solution to produce the desired test concentrations were given into the water and were homogeneously distributed. The algae were then transfered into the flasks. The test solution was homogeneously distributed in the test vessels at all test times from 0.02 - 0.07 mg/l. Deposit and small parts of the test substance were swimming at the surface of the test vessels at all test times from 0.4 - 27.1 mg/l.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green Algae (Scenedesmus subspicatus)
- Source (laboratory, culture collection): CIBA-GEIGY Ltd., testing facility
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21 - 25 °C +/- 1 °C
- pH:
- 7.3 - 7.7
- Nominal and measured concentrations:
- nominal:
1.23, 3.7, 11.1, 33.3, 100 mg test substance / l
measured:
0.02, 0.07, 0.4, 1.4, 27.1 mg test substance /l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml Erlenmeyer flasks, on Lab-shaker, 50 ml test solution per flask
- Initial cells density: 4800 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Light intensity and quality: Continous illumination, cold white fluorescent light, 120 µE/m2 sec +/- 20% (approx. 8000 lux)
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 27.1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Any other information on results incl. tables
Inhibition data
Conc. Actual end [mg/l] |
A 1 |
A 2 |
A 3 |
A 4 |
A 5 |
A 6 |
Mean A |
Inhibition * IA 0-72h [%] |
Blank |
377 |
431 |
358 |
448 |
589 |
404 |
434 |
0.0 |
0.02 |
532 |
440 |
332 |
|
|
|
435 |
0.0 |
0.07 |
690 |
602 |
749 |
|
|
|
680 |
0.0 |
0.4 |
661 |
774 |
713 |
|
|
|
716 |
0.0 |
1.4 |
1120 |
1162 |
1564 |
|
|
|
1282 |
0.0 |
27.1 |
1722 |
1488 |
1547 |
|
|
|
1586 |
0.0 |
A = Areas under Growth Curves * 10000
* promotion of algal growth compared to control is indicated as 0.0
Analytical determination of test concentrations
Labelling of sample |
Nom. Conc. [mg/l] |
Conc. Determined [mg/l] |
Mean [mg/l] |
Percent of nominal conc. |
Day 0 |
Blank |
n.d. / n.d. |
- |
- |
Day 0 |
1.23 |
0.06 / 0.08 |
0.07 |
5.7 |
Day 0 |
3.7 |
0.5 / 0.6 |
0.6 |
15.1 |
Day 0 |
11.1 |
3.4 /3.7 |
3.6 |
32.4 |
Day 0 |
33.3 |
7.6 / 8.1 |
7.9 |
23.7 |
Day 0 |
100 |
33.8 /49.1 |
41.5 |
41.5 |
Day 3 |
Blank |
n.d. / n.d. |
- |
- |
Day 3 |
1.23 |
0.01 / 0.02 |
0.02 |
1.6 |
Day 3 |
3.7 |
0.05 / 0.08 |
0.07 |
1.9 |
Day 3 |
11.1 |
0.6 / 0.2 |
0.4 |
3.6 |
Day 3 |
33.3 |
1.2 / 1.6 |
1.4 |
4.2 |
Day 3 |
100 |
30.1 / 24.1 |
27.1 |
27.1 |
n.d. = not detectable (<0.01 mg/l)
Becauee of the poor water solubility of the test substance the recoveries at start of exposure range from 5.7 t o 41.5%. A lose of test substance appeared between the highest and the lowest test concentration due to the heterogenous stock solution containing non-dissolved particles.Atthe end of exposure setteled down particles are at the origin of the 4.1 to28.8%lower recoveries.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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