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EC number: 280-489-7 | CAS number: 83567-04-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From March 15, 1999 to March 18, 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- RA study
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EC L 133, Part C: Biodegradability: Test for inhibition of oxygen consumption
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Remarks:
- direct weighing
- Details on test solutions:
- Test concentrations: 0, 100, 1000 and 10000 mg/L
The test substance has been added to about 130 mL deionized water and stirred overnight before testing (equilbrium phase) - Test organisms (species):
- activated sludge
- Details on inoculum:
- Origin: aeration tank of a waste water plant treating predominantly domestic sewage (Wupper area water authority)
Pretreatment: none
Test concentration of the activated sludge: 560 mg/L
pH of the suspension before application: 6.8 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +/- 2°C
- pH:
- 7.1 - 8.2
- Nominal and measured concentrations:
- 0, 100, 1000 and 10000 mg/L (nominal)
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (purity: 97%; stock solution: 500 mg/L) at concentrations of 5, 10 and 20 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- There was no physico-chemical oxygen consumption at the highest test substance concentration, therefore no physico-chemical oxygen consumption has been determined at the lower concentrations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the nominal 3 h EC50 of the substance in activated sludge was determined to be greater than 10000 mg/L.
- Executive summary:
A study was conducted to determine the toxicity of the read-across substance to the respiration of activated sludge microorganisms according to a method equivalent to OECD Guideline 209. Activated sludge (at 560 mg/L suspended solids level) was exposed to the test substance at nominal concentrations of 0, 100, 1000 and 10000 mg/L for 3 h. The reference substance3,5 -dichlorophenol was run in parallel at 5, 10 and 20 mg/L. Temperature and pH were recorded at test end. Under the study conditions, the nominal 3 h EC50 of the substance in activated sludge was determined to be greater than 10000 mg/L (Müller, 1999).
Reference
Table 1. Results for the test substance
Test concentration (mg/L) | Respiratory rate test substance (mg/L*h) | Phys. chem. O2 -consumption (mg/L*h) | Respiratory rate - phys. chem. O2 - consumption (mg/L*h) | Inhibition (%) |
100 | 27.8 | 0.0 | 27.8 | 1.1 |
1000 | 28.3 | 0.0 | 28.3 | 0.0 |
10000 | 24.8 | 0.0 | 24.8 | 11.7 |
Control mean: 28.1 mg/L*h (respiratory rate)
Table 2. Results for the reference substance
Test concentration (mg/L) | Respiratory rate (mg/L*h) | Inhibition (%) | |
5 | 22.3 | 20.6 | |
10 | 15.0 | 46.6 | |
20 | 6.8 | 75.8 |
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
- EC10 or NOEC for microorganisms:
- 10 000 mg/L
Additional information
A study was conducted to determine the toxicity of the read-across substance to the respiration of activated sludge microorganisms according to a method equivalent to OECD Guideline 209. Activated sludge (at 560 mg/L suspended solids level) was exposed to the test substance at nominal concentrations of 0, 100, 1000 and 10000 mg/L for 3 h. The reference substance 3,5 -dichlorophenol was run in parallel at 5, 10 and 20 mg/L. Temperature and pH were recorded at test end. Under the study conditions, the nominal 3 h EC50 of the substance in activated sludge was determined to be greater than 10000 mg/L (Müller, 1999).
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