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EC number: 204-541-5 | CAS number: 122-40-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journal.
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- The Minimum Inhibition (MIC) effect of test chemical alpha amyl cinnamaldehyde was observed on Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): alpha-Amyl cinnamaldehyde
- Molecular formula : C14H18O
- Molecular weight : 202.2952g/mole
- Smiles notation : c1(\C=C(\CCCCC)C=O)ccccc1
- InChl : 1S/C14H18O/c1-2-3-5-10-14(12-15)11-13-8-6-4-7-9-13/h4,6-9,11-12H,2-3,5,10H2,1H3/b14-11-
- substance type: organic
- physical state: liquid - Analytical monitoring:
- not specified
- Details on sampling:
- Sampling method:
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):dimethyl sulfoxide (DMSO). - Test organisms (species):
- other: Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC))
- Details on inoculum:
- Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) from the Department of Dermatology, University of Pennsylvania.
- Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 24 h
- Remarks on exposure duration:
- No data
- Post exposure observation period:
- No data
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- No data
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Details on test conditions:
- TEST SYSTEM
Test vessel: Agar plates
No. of organisms per vessel: microbial concentration of 106CFU/ml - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: effects conc. at inoculum 105CFU/plate for Corynebacterium minutissimum (CM),Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) species
- Key result
- Duration:
- 24 h
- Dose descriptor:
- other: MIC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: Effect concentration at inoculum of 10 6 CFU/Plate for Arthrobacter sp. isolated from Lipo-66
- Details on results:
- No data
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM),Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) micro organisms species was >2000 mg/l, and for Arthrobacter sp. isolated from Lipo-66 was 1000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to alpha amyl cinnamaldehyde.
- Executive summary:
The Minimum Inhibition (MIC) effect of test chemical Benzyl phenylacetate was observed on Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated medium were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM),Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) micro organisms species was >2000 mg/l, and for Arthrobacter sp. isolated from Lipo-66 was 1000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to alpha amyl cinnamaldehyde.
Reference
Description of key information
The Minimum Inhibition (MIC) effect of test chemical Benzyl phenyl acetate was observed on Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated medium were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C. The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) microorganisms species was >2000 mg/l, and for Arthrobacter sp. isolated from Lipo-66 was 1000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to alpha amyl cinnamaldehyde.
Key value for chemical safety assessment
Additional information
Based on the various experimental data and predicted data for the target chemical and RA chemicals have been reviewed to determine the toxic nature of α-Amylcinnamaldehyde, (2-benzylideneheptanal) 122-40-7 on the microorganisms, by using the read across chemicals RA 104-55-2 cinnamaldehyde and predicted data from QSAR toolbox version 3.4. The studies are as mentioned below:
Experiment according to the key study (1993), for α-Amylcinnamaldehyde, 122-40-7,The Minimum Inhibition (MIC) effect of test chemical Benzyl phenyl acetate was observed on Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66, Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) for exposure period of 24 hrs. Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated medium were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM), Staphylococcus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) microorganisms species was >2000 mg/l, and for Arthrobacter sp. isolated from Lipo-66 was 1000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to alpha amyl cinnamaldehyde.
Similarly in the another supporting study as per the OECD QSAR toolbox version 3.4 for the target chemicalα-Amylcinnamaldehyde (122-40-7), Based on the prediction done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on microorganisms was predicted for alpha-Amyl cinnamaldehyde (CAS: 122-40-7). IGC50 value was estimated to be 15mg/l when alpha-Amyl cinnamaldehyde was exposed to Tetrahymena pyriformis for 48 hours.
By considering another study for the RA chemical Cinnamaldehyde (104-55-2), 2009 The Minimum Inhibition (MIC) effect of test chemical Cinnamaldehyde was observed on E. coli CGMCC 1.487 for exposure period of 24 hrs. By using Broth macro-dilution assay. 3200, 1600, 800, 400, 200, and 100 mg/L concentrations of chemical was added to MHB to obtain a stock solution of 3200 mg/L with Tween-80 as emulsifier culture Was maintained on Mueller–Hinton agar (MHA) (Beijing Chemical Corp., Beijing, China) slants at 4 ◦C. Cells were prepared by 16-h culture in Mueller–Hinton broth (MHB) (Beijing Chemical Corp.) at 37 ◦C. A 16-h culture was diluted with MHB, inoculum 1 × 106CFU/mL was added. The Minimum Inhibition (MIC) effect of test chemical Cinnamaldehyde on E. coli CGMCC 1.487 was 400mg/l.
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