Trisodium 5-[[4-chloro-6-(ethylphenylamino)-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(4-methyl-2-sulphonatophenyl)azo]naphthalene-2,7-disulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11.5.2000 - 29.6.2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: Test No. L 47-Fish, Acute Toxicity test. Chemical formulation and waste waters

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Test organisms (species):

Poecilia reticulata

Details on test organisms:

TEST ORGANISM
- Common name: Poecila reticulata
- Source: AKVA-SALON, 503 02 Neděliště 51
- Age at study initiation: 3-4 months
- Length at study initiation: 2-3 cm
- Feeding during test: no


ACCLIMATION
- Acclimation: yes
- Acclimation conditions: same as test
- Feeding frequency: 72h before test without feeding

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

22.0 ± 1.0 °C

pH:

7.8

Nominal and measured concentrations:

100.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquarium, volume of 10 L
- Type: Open
- Aeration: No
The dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1 000 mL of distilled water.
A. 117.6 g CaCl2.2H2O in 1 L of distilled water
B. 49.3 g MgSO4.7H2O in 1 L of distilled water
C. 25.9 g NaHCO3 in 1 L of distilled water
D. 2.3 g KCl in 1 L of distilled water

The stock solution: 0.1 g/L
The tested concentration: 100.2 mg/L
The volume of the test solution was 1 000 in the preliminary test and 4 000 mL in the limit test.

Preliminary test: 3 ks animals (in the one beaker – no replicates)
Verification test: 10 ks animals (in the one beaker – no replicates)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: not observed
- Observations on body length and weight: not observed
- Other biological observations:not observed
- Mortality of control: not observed


The measured values of pH solution should not change more than 1 pH unit.

Validity criteria fulfilled:

yes

Remarks:

The measured values of pH solution should not change more than 1 pH unit.

Conclusions:

The test substance, Reactive red 45:1, was tested for acute toxicity on fish Poecilia reticulata The test was performed according to method est No. L 47-Fish, Acute Toxicity test. Chemical formulation and waste waters. The test was performed as static. No mortality was observed.

Description of key information

Reactive Red 45:1 caused no mortality in Poecilia reticulata at 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L

Additional information

The test substance, Reactive Red 45:1, was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47-Fish, Acute Toxicity test. Chemical formulation and waste waters. The test was performed as static. No mortality was observed at 100 mg/L.