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EC number: 228-995-9 | CAS number: 6388-26-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From March 14 to April 3, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- GLP compliance:
- no
- Remarks:
- in accordance with ISO/IEC 17025
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Test medium: synthetic sewage feed prepared with tap water: 16 ml of 100-fold sewage concentrate per 500 ml of final test mixture
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - inoculum: activated sludge from aeration tank of a municipal biological waste water treatment plant, non adapted, non pre-conditioned
- source: ARA Werdhölzli, CH-8048 Zürich (28 March 2007, 11 a.m.)
- concentration: 1.6 g/l dry matter in the final mixture - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 22 ± 0.5 °C
- pH:
- 7.5 ± 0.5
- Nominal and measured concentrations:
- Nominal concentrations: 125, 250, 500, 1000 and 2000 mg/l.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 ml flask
- Fill volume: 500 ml
- Aeration: yes
- No. of vessels per concentration: 1
- No. of vessels per control: 2
- Sludge concentration: 1.6 g/l dry matter
- Nutrients provided for bacteria: synthetic sewage feed
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
OTHER TEST CONDITIONS
- Adjustment of pH: to 7.5 ± 0.5 with NaOH or H2SO4, if necessary
EFFECT PARAMETERS MEASURED: oxygen consumption
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: no - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- No significant toxic effects to activated sludge were noted; respiration rates were in the same range as detremined for the 2 blank controls.
Variations between 2 controls was ± 5 %. - Results with reference substance (positive control):
- EC50 (3h) = 4.6 mg/l
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (3h) > 2000 mg/l
- Executive summary:
Method
Inhibitory effect of test substance on oxygen consumption of activated sludge microorganisms of a municipal treatment plant was tested under aerobic static conditions after 3 h of exposure. The inhibitory effect may include the effect on respiration and nitrification.
Nominal concentrations tested were 125, 200, 500, 1000 and 2000 mg/l, prepared by dilution of stock solution. No chemical analysis of test concentrations was conducted.
Results
No significant toxic effects were noted upon 3 h of exposure. Respiration rates were in the same range as determined for 2 blank controls. EC20, EC50 and EC80 > 2000 mg/l.
Reference
nominal concentration (mg/l) | O2 consumption rates (mg O2/l/h) | respiration inhbition (%) |
0 | 31.0 | - |
0 | 34.2 | - |
125 | 32.9 | -0.9 |
250 | 32.6 | 0.0 |
500 | 32.9 | -0.8 |
1000 | 31.9 | 2.3 |
2000 | 30.8 | 5.7 |
reference | ||
3 | 19.4 | 40.4 |
10 | 10.6 | 67.4 |
30 | 4.8 | 85.3 |
Description of key information
Respiration rate: EC50 (3h) > 2000 mg/l.
Key value for chemical safety assessment
Additional information
TThe substance was tested according to OECD guideline 209.
Toxicity to microorganisms was evaluated by considering oxygen consumption upon 3 h of static exposure at concentrations between 125 and 2000 mg/l.
Two further studies were available, dated 1975 and 1982 and lacking of details on procedures and results. In both cases, no toxic effect were noted at the tested concentrations of 300 mg/l and 10000 mg/l, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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