Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-471-2 | CAS number: 51850-20-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant, guideline study, available as unpublished report, no restrictions, fully adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Hexachloroplatinic acid
- EC Number:
- 241-010-7
- EC Name:
- Hexachloroplatinic acid
- Cas Number:
- 16941-12-1
- Molecular formula:
- Cl6Pt.2H
- IUPAC Name:
- Hexachloroplatinic acid
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were taken from the control and the treatment groups (replicates R1 to R2 pooled) and the 0.010, 0.032, 0.10, 0.32 and 1.0 mg test item/L at 0 and 48 hours for quantitative analysis. Duplicate samples and samples of the 0.018, 0.056, 0.18 and 0.56 mg test item/L test groups were taken and stored frozen (approximately - 20 °C) for further analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: an amount of test material (100 mg) was dissolved in reconstituted water and the volume adjusted to 1 L to give a 100 mg/L stock solution from which a serial dilution was prepared to give a 1.0 mg/L stock solution. Aliquots (10, 18, 32, 56, 100, 180, 320 and 560 mL) of the 1.0 mg/L stock solution were each separately dispersed in reconstituted water and the volume adjusted to 1 L to give the remainder of the test series of 0.010, 0.018, 0.032, 0.056, 0.10, 0.18, 0.32 and 0.56 mg test item/L, respectively. Each stock solution and prepared concentration was inverted several times to ensure adequate mixing and homogeneity.
- Controls: dilution water without test item
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none - clear and colourless solutions throughout the duration of the study were reported for each test concentration.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: in-house laboratory cultures
- Age of test organisms: first instar, less than 24 hours od
- Feeding during test: none
ACCLIMATION
- Acclimation period: none
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
- Adult daphnids were maintained in polypropylene vessels containing approximately 2 L of reconstituted water (same as test) in a temperature controlled room at 21 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20-minute dawn and dusk transition periods. Each culture was fed with a suspension of algae (Chlorella sp.). Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young daphnids were removed from the cultures and used for testing.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 21°C throughout the test
- pH:
- 8.0 throughout the test
- Dissolved oxygen:
- 8.3 - 8.5 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 0.010, 0.018, 0.032, 0.056, 0.10, 0.18, 0.32, 0.56 and 1.0 mg test item/L
Measured concentrations within 88-112% of nominal, so considered justified to express all as nominal concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250-mL covered glass jars, containing 200 mL of test solution
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water prepared from four stock solutions: a) CaCl2.2H2O 11.76 g/L, b) MgSO4.7H2O 4.93 g/L, c) NaHCO3 2.59 g/L, d) KCl 0.23 g/L. An aliquot (25 mL) of each of the four stock solutions a-d was added to each L (final volume) of deionised water with a conductivity of < 5 μS/cm. The reconstituted water had a pH of 7.8 ± 2 °C adjusted (if necessary) with NaOH or HCl and was aerated until the dissolved oxygen concentration was approximately air-saturation value.
- Total Hardness: 250 mg/L as CaCO3
- Conductivity: < 5 μS/cm
- Intervals of water quality measurement: water temperature was recorded daily throughout the study. Dissolved oxygen concentrations and pH were recorded at the start and at the end of the test.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness with 20-minute dawn and dusk transition periods
EFFECT PARAMETERS MEASURED: any immobilisation or adverse reactions to exposure were recorded at 24 and 48 hours of exposure. Organisms were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.
RANGE-FINDING STUDY
- Test concentrations: 0.010, 0.10, 1.0, 10 and 100 mg test item/L (nominal concentrations)
- Results used to determine the conditions for the definitive study: no immobilisation was observed at the test concentration of 0.010 mg test item/L. However, immobilisation was observed at 0.10, 1.0, 10 and 100 mg test item/L. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % Confidence Interval: 0.11 - 0.16 mg test item/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.052 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % Confidence Interval: 0.044 - 0.064 mg Pt/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.056 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.022 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- element
- Remarks:
- Pt
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: not reported
- Reported statistics and error estimates:
- EC50 values at 24 and 48 hours of exposure were determined by Probit analysis (Finney, 1971). The NOEC was based upon zero immobilisation at the relevant concentration.
Any other information on results incl. tables
Table 1. Cumulative immobilisation data after 24 and 48 hours of exposure to hexachloroplatinic acid for Daphnia magna
Nominal concentration (mg test item/L) | Cumulative immobilised Daphnia (Initial population: 10 per replicate) | |||||||
24 hours | 48 hours | |||||||
Replicate 1 | Replicate 2 | Total | % | Replicate 1 | Replicate 2 | Total | % | |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0.010 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0.018 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0.032 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0.056 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0.10 | 0 | 0 | 0 | 0 | 3 | 5 | 8 | 40 |
0.18 | 0 | 0 | 0 | 0 | 7 | 6 | 13 | 65 |
0.32 | 3 | 2 | 5 | 25 | 10 | 10 | 20 | 100 |
0.56 | 5 | 5 | 10 | 50 | 10 | 10 | 20 | 100 |
1.0 | 10 | 7 | 17 | 85 | 10 | 10 | 20 | 100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-hour EC50 of hexachloroplatinic acid to Daphnia magna was determined to be 0.13 mg test item/L, corresponding to 0.052 mg Pt/L. The 48-hour NOEC was determined to be 0.056 mg test item/L (0.022 mg Pt/L).
- Executive summary:
A 48-h acute immobilisation Daphnia magna study is available for hexachloroplatinic acid. The study is reliable without restrictions, being GLP-compliant and having followed the standard test guidelines (OECD 202 and EU Method C.2).
A static test system was followed. Nine test concentrations were used and these were analysed using UV-Adsorptive stripping voltammetry. Measured test concentrations ranged from 88 % to 112 % of nominal, and therefore results were based on nominal concentrations. The 48-hour EC50 was determined to be 0.13 mg test item/L, corresponding to 0.052 mg Pt/L. The 48-hour NOEC was determined to be 0.056 mg test item/L (0.022 mg Pt/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.