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EC number: 246-905-6 | CAS number: 25371-55-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7 September 2005 to 6 October 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl hydrogen octadecylphosphonate
- EC Number:
- 246-905-6
- EC Name:
- Methyl hydrogen octadecylphosphonate
- Cas Number:
- 25371-55-5
- Molecular formula:
- C19H41O3P
- IUPAC Name:
- methoxy(octadecyl)phosphinic acid
- Reference substance name:
- Octadecylphosphonic acid
- EC Number:
- 225-216-4
- EC Name:
- Octadecylphosphonic acid
- Cas Number:
- 4724-47-4
- Molecular formula:
- C18H39O3P
- IUPAC Name:
- octadecylphosphonic acid
- Reference substance name:
- Dimethyl octadecylphosphonate
- EC Number:
- 246-904-0
- EC Name:
- Dimethyl octadecylphosphonate
- Cas Number:
- 25371-54-4
- Molecular formula:
- C20H43O3P
- IUPAC Name:
- dimethyl octadecylphosphonate
- Test material form:
- solid
- Details on test material:
- Test Item Identification: X#15249Batch: 04055A001Purity: >91%Description: White waxy solid blockStorage conditions: room temperature in the dark
Constituent 1
impurity 1
impurity 2
- Specific details on test material used for the study:
- Test Item Identification: X#15249Batch: 04055A001Description: White waxy solid blockStorage conditions: room temperature in the dark
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: A mixed population of activated sewage sludge micro-organisms was obtained on 6 September 2005 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.- Preparation of Inoculum: The sample was washed two times by settlement and resuspension in mineral medium to remove any excessive amounts of dissolved organic carbon (DOC) that may have been present. The washed sample was then maintained on continuous aeration in the laboratory at a temperature of approximately 21 °C and used on the day of collection. Determination of the suspended solids level of the activated sewage sludge was carried out by filtering a sample (100 mL) of the washed activated sewage sludge by suction through pre-weighed GF/A filter paper using a Buchner funnel (rinsed three times with 10 mL deionised reverse osmosis water prior to drying in an oven). Filtration was then continued for a further 3 minutes after rinsing the filter three successive times with 10 mL of deionised reverse osmosis water. The filter paper was then dried in an oven at approximately 105 °C for at least 1 hour and allowed to cool before weighing. This process was repeated until a constant weight was attained. The suspended solids concentration was equal to 2.0 g/L prior to use.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 15.1 mg/L
- Based on:
- test mat.
- Remarks:
- equivalent to 10mgC/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- PRELIMINARY INVESTIGATIONAL WORKPreliminary investigational work determined that the substance was not soluble in water. Methods to improve solubility/dispersibility included ultrasonication, with & without heating to 65 C and high shear mixing, following ultrasonication and on its own. All methods formed a cloudy water column with large wax pieces of test material visible on the surface. As there was no difference in the observations made on the preparations between high shear mixing and ultrasonication, it was considered appropriate to use ultrasonication as a means to aid the dispersion of the test material.EXPERIMENTAL PREPARATION45.3 mg of test material was dispersed in 200 ml of culture medium and subjected to ultrasonification for 30 minutes. The volume was adjusted to 3 litres to give a final test item concentration of 15.1 mg/L, equivalent to 10 mg carbon/L. Inoculum control vessels were prepared with inoculated mineral medium and the vessels were adjusted to 3 litres with mineral media. The final concentration of suspended solids was 30 mg/L.STANDARD MATERIALSodium benzoate was used as the standard material. An initial stock solution of 1000 mg/L was prepared by dissolved the standard material directly in the culture medium. A 51.4 mL aliquot was added to the test vessel and diluted to give a final concentration of 17.1 mg/L or 10 mg carbon/L. The solution was inverted several times to ensure homogenity of the solution.TOXICITY CONTROLA toxicity control, containing the test and standard material, was prepared in order to assess any toxic effect of the test material on the sewage sludge micro-organisms.An aliquot (453.3 mg) of the test material stock solution was dispersed in 200 ml of cultured medium and subjected to ultrasonification for 30 minutes. An aliquot (51.4 mL) of sodium benzoate stock solution was also added to the test vessel. The volume was adjusted to 3 litres to give a final concentration of 15.1 mg test material/L plus 17.1 mg sodium benzoate/L, equivalent to a total of 20 mg carbon/L.PREPARATION OF TEST SYSTEMThe following preparations were made up and inoculated in 5 litre test culture vessels each containing 3 litres of solution:a) An inoculated control, in duplicate, consisting of inoculated mineral medium.b) The procedure control containing the reference material, in duplicate, in inoculated mineral medium to give a final concentration of 10 mg carbon/L.c) The test material, in duplicate, in inoculated mineral medium to give a final concentration of 10 mg carbon/L.d) The test and reference materials in inoculated mineral medium to give a final concentration of 20 mg carbon/L to act as toxicity control (one vessel only).Each test vessel was inoculated with the prepared inoculum at a final concentration of 2.5 mg suspended solids (ss)/L. The concentration of the suspended solids was determined by filtering a sample of washed activated sludge through pre-weighed GF/A filter paper and weighing the filter paper after drying in an oven at 105 oC for at least 1 hour. The test was carried out in a temperature controlled room at 22 ± 2 °C in darkness.Approximately 24 hours prior to addition of the test and reference materials, the vessels were filled with 2400 mL of mineral medium and 36 mL of inoculum and aerated overnight. On Day 0 the test and reference materials were added and the volume in all the vessels was adjusted to 3 litres by the addition of culture medium .The test vessels were sealed and CO2-free air bubbled through the solution at a rate of 40 mL/min per vessel and stirred continuously by magnetic stirrer.The CO2 produced by degradation was collected in two 500 mL Dreschel bottles containing 350 mL of 0.05 M NaOH. The CO2 absorbing solutions were prepared using purified de-gassed water.pH MEASUREMENTSThe pH of the test preparations was determined on day 28 prior to acidification with hydrochloric acid, using a WTW pH/Oxi 340I pH and dissolved oxygen meter.VALIDATION CRITERIA-The results are considered valid if, in the same test, the reference material yields 60 % degradation by day 14.-The test material may be considered to be readily biodegradable if ≥60 % degradation is attained within 28 days. This level of degradation must be reached within 10 days of biodegradation exceeding 10 %.-The toxicity control should attain ≥25 % degradation by day 14 for the test material to be considered as non-inhibitory.-The test is considered valid if the difference of the extremes of replicate values of production of CO2 is less than 20 %.-The total CO2 evolution in the control vessels at the end of the test should not normally exceed 40 mg/L medium-The IC content of the test material suspension in the mineral medium at the beginning of the test should be <5 % of the TC.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Preliminary study:
- The results obtained from the preliminary work proved the substance was not soluble in water.
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 43
- Sampling time:
- 28 d
- Remarks on result:
- other: The pH values for the two replicates R1 and R2 were 7.6 and 7.7, respectively, on day 28.
- Details on results:
- The total CO2 evolution in the inoculum control vessels on day 28 was 23.06 mg/L, satisfying the validation criteria of the OECD guidelines. The IC/TC ratioof the test material in suspension in the mineral mdicium at the start of the test was below 5%, satisfying the validation criteria of the OECD guidelines.The toxicity control attained 56 % degradation after 14 days and 65 % degradation after 28 days thereby confirming that the test material was not toxic/ inhibitory to the sewage treatment micro-organisms used in the test.Sodium benzoate attained 62 % degradation after 14 days and 78 % degradation after 28 days thereby confirming the suitability of the inoculum and test conditions.The test material attainted 43% degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms of OECD Guideline 301B.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- Under the conditions of this study, the test material attained 43 % degradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD guideline 301B,.
- Executive summary:
The ready biodegradability of the test material was assessed in accordance with the standardised guideline OECD 301B.
The test material, at a concentration of 10 mg Carbon/L, was exposed (in an aerobic aqueous medium) to activated sewage sludge microorganisms with mineral medium in sealed culture vessels in the dark at 21 °C for 28 days.
The degradation of the test material was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference material, sodium benzoate, together with a toxicity control were used for validation purposes.
Under the conditions of this study, the test material attained 43 % degradation after 28 days and therefore cannot be considered to be readily biodegradable u nder the strict terms and conditions of OECD guideline 301B.
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