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EC number: 243-325-5 | CAS number: 19800-42-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening test, other
- Remarks:
- The substance was tested in an inherent biodegradation study
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From November 17, 1987 to November 26, 1987
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.9 (Biodegradation: Zahn-Wellens Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Test procedures:
- DOC (DIN 38 409 part 3)
- CSB (EEC 84/449 and DIN 38 409 part 41) - GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): wastewater treatment plant of Kelsterbach/Hoechst 70:30
- 12 g/L centriguged sludge equivalent to ca. 1263 mg/L dry weight - Duration of test (contact time):
- 28 d
- Initial conc.:
- 201 other: mg C/L
- Based on:
- DOC
- Initial conc.:
- 767 other: mg O2/L
- Based on:
- other: CSB
- Parameter followed for biodegradation estimation:
- DOC removal
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 59
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Executive summary:
A study was conducted to determine the biodegradability of the test substance (43.7% purity) in accordance with OECD Guideline 302 B and EU Method C.9 (Zahn-Wellens test). Non-adapted wastewater treatment plant sludge was exposed to the equivalent of 201 mg C/L of substance under aerobic conditions. Removal of dissolved organic carbon (DOC) was monitored for 28 days. A biodegradation of 59% was observed by test end. The substance is therefore considered to be inherently biodegradable under the conditions of the Zahn-Wellens test (Voelskow, 1989).
Reference
| Time | Elimination (%) according to DIN criteria | Biodegradation (%) according to EU criteria |
| 3 h | 24 | 0 |
| 1 d | 31 | 10 |
| 7 d | 50 | 35 |
| 10 d | 50 | 35 |
| 15 d | 51 | 36 |
| 28 d | 68 | 59 |
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
A study was conducted to determine the biodegradability of the test substance (43.7% purity) in accordance with OECD Guideline 302 B and EU Method C.9 (Zahn-Wellens test). Non-adapted wastewater treatment plant sludge was exposed to the equivalent of 201 mg C/L of substance under aerobic conditions. Removal of dissolved organic carbon (DOC) was monitored for 28 days. A biodegradation of 59% was observed by test end. The substance is therefore considered to be inherently biodegradable under the conditions of the Zahn-Wellens test (Voelskow, 1989).
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