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EC number: 214-684-5 | CAS number: 1185-53-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
A short-term study with TRIS HCl is available for daphnia magna (6.1.3-1). Since data for toxicity to algae and fish were not available for assessment, a read-across approach from TRIS AMINO (6.1.5-1) and AEPD (6.1.1-1) were used. TRIS AMINO and AEPD are both soluble in water, non-volatile and have low potential for adsorption, thus their main target environmental compartment is water. All tests demonstrate low aquatic toxicity, with LC/EC50 values >100 mg/L for fish, daphnia and alga, respectively.
AEPD, TRIS AMINO and TRIS HCl are well soluble in water (> 600 g/L) and have a very low vapour pressure (< 0.01 Pa). The low partition coefficient (log Kow) of -2.31 (TRIS AMINO), -1.02 (AEPD) and -3.6 (TRIS HCl) indicate low potential to accumulate in biological systems. Additionally, AEPD and TRIS AMINO are rapidly biodegradable. In the ready biodegradation test (301F), TRIS AMINO reached 100% degradation within 28 days and AEPD was rapidly degraded in the inherent test (> 90% with 7 days).
The read-across from AEPD and TRIS AMINO to TRIS HCl for the endpoints short-term toxicity to fish and toxicity to algae is justified by the data comparison discussed above as well as by structural and physico-chemical similarity of the two substances.
Furthermore, both OASIS and ECOSAR classification models indicate narcosis as mode of action for TRIS HCl, TRIS AMINO and AEPD. However, it is known that for amines the toxicity can be enhanced with respect to baseline, and the mode of action can be classified as “amine narcosis”. Nevertheless, as in the general case of narcosis (i.e. with absence of specific and reactive effects – what is confirmed by the presence of only amine and alcohol functional groups), log Kow is the main toxicity trigger. This descriptor takes very low values for both substances as discussed before and this fact is reflected in high experimental acute toxicity values for fish, Daphnia and algae.
OECD QSAR Toolbox (v. 3.3.0) profiling in regard to aquatic toxicity is presented in the table below.
Table: OECD QSAR Toolbox characteristics of environmental toxicity profiles of target and source substances
Profiling | Target chemical, TRIS HCl CAS 1185-53-1 |
Source chemical, Trometamol CAS 77-86-1 |
Source chemical, AEPD CAS 115-70-8 |
Acute aquatic toxicity classification by Verhaar (modified) | Class 5 (not possible to classify according to these rules) | Class 5 (not possible to classify according to these rules) | Class 5 (not possible to classify according to these rules) |
Acute aquatic toxicity MOA by OASIS | Narcotic amine | Narcotic amine | Narcotic amine |
Aquatic toxicity classification by ECOSAR | Aliphatic amine | Aliphatic amine | Aliphatic amine |
Organic functional groups (nested) | Alcohol, aliphatic amine (primary) | Alcohol, aliphatic amine (primary) | Alcohol, aliphatic amine (primary) |
Table: Environmental fate and toxicity
Endpoint |
Target chemical, TRIS HCl CAS 1185-53-1 |
Source chemical, Trometamol CAS 77-86-1 |
Source chemical, AEPD CAS 115-70-8 |
Biodegradation |
RA CAS 77-86-1 readily biodegradable |
readily biodegradable | rapidly biodegradable |
Adsorption, log Koc |
no data available | 1.87 (QSAR) |
2.96 (QSAR) |
Short-term toxicity to fish, 96h-LC50, mg/L | RA CAS 115-70-8 460 |
RA CAS 115-70-8 460 |
460 |
Short-term toxicity to daphnia, 48h-EC50, mg/L | > 117 | > 980 | 668 |
Toxicity to algae, 72h-EC50, mg/L | RA CAS 77-86-1 397 |
397 | 548 |
Toxicity to algae, 72h-NOEC, mg/L | RA CAS 77-86-1 100 |
100 | 31.3 |
Toxicity to microorganisms, EC50, mg/L | > 1000 (3 h) | > 1000 (3 h) | 640 |
Toxicity to microorganisms, EC10, mg/L | > 1000 (3 h-NOEC) | > 1000 (3 h-NOEC) | 300 |
Terrestrial studies are not available for TRIS HCl or for potential analogue substances. However, the substances are expected to partition mainly into the aquatic compartment and to be removed quickly due to rapid biodegradation. Additionally, significant bioaccumulation is not likely due to the low Kow, and the data available for aquatic organisms indicates low toxicity concern.
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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