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EC number: 238-744-5 | CAS number: 14694-95-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Toxicity test: January 4 1989; Mutagenicity assay: January 25-January 30 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study was GLP compliant and was produced largely in accordance with the relevant OECD guideline. However, the study report omitted the following information: purity of the test substance, justification for the choice of solvent, and historical negative and positive control data. In addition, the study did not include a tester strain suitable for the detection of cross-linking mutagens, as recommended in OECD Guideline 471.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- the study did not include a tester strain suitable for the detection of cross-linking mutagens, as recommended in OECD Guideline 471
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tris(triphenylphosphine)rhodium (I) chloride
- EC Number:
- 238-744-5
- EC Name:
- Tris(triphenylphosphine)rhodium (I) chloride
- Cas Number:
- 14694-95-2
- Molecular formula:
- C54H45ClP3Rh
- IUPAC Name:
- tris(triphenylphosphine)rhodium (I) chloride
- Details on test material:
- - Name of test material (as cited in study report): Tris-(triphenylphosphan)-Rhodium(I)-chlorid
- Substance type: solid
- Physical state: magenta-red crystalline solid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components:
- Isomers composition:
- Purity test date: no data
- Lot/batch No.: 6868-4
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: ambient temperature
- Other: Test substance was received from Degussa AG on December 13 1988
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9
- Test concentrations with justification for top dose:
- 12.3, 37, 111.1, 333.3, and 1000µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: [DMSO (prepared “under ultrasonic wave”]
- Justification for choice of solvent/vehicle: no data
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: sodium azide 2-nitrofluorene 9-aminoacridine other: [2-aminoanthracene]
- Remarks:
- see below
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: three days
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other:
OTHER:
A preliminary toxicity test was carried out with six concentrations of the test substance in S. typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100.
Appropriate strain-specific positive controls were used in the absence of S9 metabolic activation. In the presence of S9 metabolic activation, 2-aminoanthracene was used as the sole positive control in all strains tested. The batch of S9 used was characterised in tests with the mutagens 4-aminobiphenyl and 2-aminoanthracene. - Evaluation criteria:
- A positive response was indicated by a two-fold or greater increase in the mean number of revertant colonies appearing on the test plates over and above the background spontaneous reversion rate observed on the vehicle control plates, together with evidence of dose response.
- Statistics:
- No statistical analysis were presented in the report.
Results and discussion
Test results
- Key result
- Species / strain:
- bacteria, other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: no data
- Effects of osmolality: no data
- Evaporation from medium: no data
- Water solubility: no data
- Precipitation: In the preliminary toxicity test, the test substance precipitated in the plates at dose levels of 1 and 10 mg/plate.
- Other confounding effects: no data
RANGE-FINDING/SCREENING STUDIES: A preliminary toxicity study was performed with the S. typhimurium tester strains.
COMPARISON WITH HISTORICAL CONTROL DATA: no data
ADDITIONAL INFORMATION ON CYTOTOXICITY: The results of a preliminary toxicity test found that 10 mg/plate was toxic in all tester strains both in the presence and absence of S9. At 1 mg/plate, slight toxicity was noted for TA 1535, TA 1537 (both in the presence and absence of S9), TA 1538 (in the absence of S9 only) and TA100 (in the presence of S9 only). Toxicity was not observed at dose levels of 0.1 mg/plate or lower.
Any other information on results incl. tables
Results were confirmed in an independent repeat of the mutation assay.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
“Tris-(triphenylphosphan)-Rhodium(I)-chlorid” displayed no evidence of mutagenic activity when tested at up to cytotoxic levels in Salmonella typhimurium strains TA 1535, TA 1537, TA 1538, TA 100 or TA 98, either in the presence or in the absence of a rat liver metabolic activation system (S9). - Executive summary:
In an OECD Test Guideline 471 study, conducted according to GLP, “tris-(triphenylphosphan)-rhodium(I)-chlorid” was examined for the ability to induce gene mutations in Salmonella typhimurium (strains TA 1535, TA 1537, TA 1538, TA 98, and TA 100) in the presence and absence of Aroclor-induced rat liver metabolic activation system (S9). Tester strains were exposed to five concentrations (ranging from 12.3 to 1000 µg/plate), based on observed precipitation in a range-finding study, with tests performed in triplicate using the plate incorporation method. No evidence of mutagenic activity was seen, either in the presence or absence of S9, in tests with up to cytotoxic levels. The results were confirmed in a repeat experiment.
This study was largely conducted in accordance with current OECD guidelines. However, some information was omitted from the study report, including: the purity of the test substance, justification for the choice of solvent, and historical negative and positive control data. The guidelines also indicate that it is preferable to include tester strains that are able to detect certain oxidising mutagens and/or cross-linking agents (e.g. Salmonella typhimurium TA102) or to include a DNA repair-proficient strain of Escherichia coli.
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