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EC number: 263-417-9 | CAS number: 62121-75-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-01-09 to 1991-01-18
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- other: "Letale Wirkung beim Zebrabärbling Brachydanio rerio", Recommeded Test Guideline from the German Federal Environment Agency
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all
- Sampling method: 100 mL taken from the middle of the aquaria at 48 and 96 h - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stirring of test item in dilution water (ISO/DIS 7346/1), homogenisation with Ultra-Turrax
- Controls: negative
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, 3422 Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): mean: 102 d
- Length at study initiation (length definition, mean, range and SD): 2.6 - 3-3 cm, mean: 2.9 cm, SD: 0.27 cm
ACCLIMATION
- Acclimation period: min. 14 d
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: TetraMin, ad libitum
- Feeding frequency during acclimation: twice per day
- Health during acclimation (any mortality observed): no mortality
FEEDING DURING TEST
- Amount: No feeding - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 21.2 - 22.9°C
- pH:
- 7.9 - 8.3
- Dissolved oxygen:
- 8.0 - 9.6 mg/L
- Nominal and measured concentrations:
- Nominal: 0 (control), 500 mg/L
Measured: No measurements for control; 500 mg/L (0 h), 500 mg/L (48 h), 499 mg/L (96 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Material, size, headspace, fill volume: volume 10L, glass, 30 x 22 x 24 cm³
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water according to ISO/DIS 7346/1
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Adjustment of pH: no, pH in dilution water was 8.2 - 8.3
- Photoperiod: 12/12
- Light intensity: 700 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, behaviour after 4, 24, 48, 72 and 96 h
TEST CONCENTRATIONS: limit test with 500 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: no mortality observed
- Details on results:
- - Behavioural abnormalities: reduced activity (all fish, 4 h); tail-orientated swimming (one fish, 4 h; some fish, 72 h); increased shock reaction (some fish, 72 h); observation was limited due to orange colour of test solution
- Mortality of control: no mortality
- Effect concentrations exceeding solubility of substance in test medium: no, solubility determined at >1000 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality was observed in a 96-h acute toxicity test with Danio rerio. The 96-h LC50 was determined >500 mg/L (analytically verified).
Reference
Description of key information
No mortality was observed in a 96-h acute toxicity test with Danio rerio. The 96-h LC50 was determined >500 mg/L (analytically verified). The results of the key study are the same as in an older supporting study performed with Leuciscus idus melanotus (96 -h LC50 > 500 mg/L).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 500 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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