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EC number: 202-544-6 | CAS number: 96-91-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from WATER RESOURCES BULLETIN
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute Toxicity of Picric Acid and Picramic Acid to Rainbow Trout, Salmo Gairdneri, and American Oyster, Crassostrea Virginica’
- Author:
- William L. Goodfellow, Jr., Dennis T. Burton, William C. Graves, Lenwood W. Hall, Jr., and Keith R. Cooper
- Year:
- 1 983
- Bibliographic source:
- Water Resources Bulletin, VOL. 19, NO. 4-641-648, AUGUST 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- Standard Methods (APHA, et al., 1981).
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2-amino-4,6-dinitrophenol
- EC Number:
- 202-544-6
- EC Name:
- 2-amino-4,6-dinitrophenol
- Cas Number:
- 96-91-3
- Molecular formula:
- C6H5N3O5
- IUPAC Name:
- 2-amino-4,6-dinitrophenol
- Test material form:
- other: Dark red needles
- Details on test material:
- - Name of test material (as cited in study report): Picramic acid
- Molecular formula (if other than submission substance): C6H5N3O5
- Molecular weight (if other than submission substance): 199.125g\mol
- Smiles notation (if other than submission substance): c1(cc(cc(c1O)N)[N+](=O)[O-])[N+](=O)[O-]
- InChl (if other than submission substance): 1S/C6H5N3O5/c7-4-1-3(8(11)12)2-5(6(4)10)9(13)14/h1-2,10H,7H2
- Substance type: Organic
- Physical state: Dark red needles
- Analytical purity: No data available.
- Lot/batch No.:38C-0117
- Storage condition of test material: The stock solutions were stored in the dark at 5°C.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Preparation of the stock solutions was accomplished by saturation of the compound in distilled water and filtration through a GN-6 Metricel 0.45 pm filter. Stock concentrations were determined spectrophotometrically at 237 nm for picramic acid.
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow Trout
- Strain: mykiss
- Source: Wytheville National Fish Hatchery, Max Meadows, Virginia.
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): (x = 41.6 _+ 8.7 mm total length
- Weight at study initiation (mean and range, SD): No data available
- Method of breeding: No data available
- Feeding during test- The fish were not fed during the 96-h assay.
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable
ACCLIMATION
- Acclimation period: 2 weeks
- Acclimation conditions (same as test or not): Yes same as test period.
- Type and amount of food: Fed commercial trout chow daily during acclimation.
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): Less than 3 percent mortality occurred in the laboratory populations of rainbow trout.
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No data available.
Test conditions
- Hardness:
- 177.0 (+ 11.9) mg/L CaCO3
- Test temperature:
- 10.8 (+0.52)°C
- pH:
- 7.9 (+ 0.04)
- Dissolved oxygen:
- Not available
- Salinity:
- Not available
- Nominal and measured concentrations:
- 0, 40, 50, 60, 70, 80, 90, and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 38 l
- Aeration: No aeration is provided.
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Renewal rate of test solution (frequency/flow rate):24 hour
- No. of organisms per vessel: Not sprecified.(25 fish)
- No. of vessels per concentration (replicates): 7 concentration (Two replicates)
- No. of vessels per control (replicates):2
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No
- Intervals of water quality measurement: Three days
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 12 hlight:12 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Analysis of the test concentrations showed that nominal and actual concentrations varied less than 2 percent.
- Test concentrations: 0, 40, 50, 60, 70, 80, 90, and 100 mg/l - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 46.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The picramic acid LC50 values were 90.9 mg/l at 24 h; 68.6 mg/l at 48 h; 56.6 mg/l at 72 h; and 46.2 mg/l at 96 h.
LC05, LC50 and LC 95 were found to be 18.5 46.2 67.8 (2.3-27.6) respectively based on 95 percent confidence limits for acute toxicity.
Applicant's summary and conclusion
- Conclusions:
- LC50 was found to be 46.2 mg/l for Picramic acid in Oncorhynchus mykiss for 96 hour.
- Executive summary:
In Short-term toxicity to fish study for Picramic acid was determined in Oncorhynchus mykiss for 96 hour. The LC50 was found to be 46.2 mg/l for Picramic acid.
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