Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 690-512-6 | CAS number: 1271172-98-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to OECD guidelines and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- ; with regards to temperature and stated as non-critical
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- ; with regards to temperatue and stated as non-critical
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction products of 1,3,5-triazine-2,4,6-triamine and zinc bis(dihydrogen phosphate)
- EC Number:
- 690-512-6
- Cas Number:
- 1271172-98-5
- Molecular formula:
- Zn(C3H7N6)2(P2O7)
- IUPAC Name:
- Reaction products of 1,3,5-triazine-2,4,6-triamine and zinc bis(dihydrogen phosphate)
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (control), 1, 3.2, 10, 32 and 100 mg/L.
- Sampling method: To all samples 1% KCl solution (10% in water) and 1% HCl conc. were added. On each day of measurements, calibrations were performed in the range 0.01 - 2 mg/L Zn.
- Frequency: The content of Zn in the test vessels was measured at the start, every 24 hours and at the end of test (72 hours).
- Sample storage conditions before analysis: Not detailed, but assumed to be analysed on the day of sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Before the experiment, saturated solutions of test substance were prepared. This was performed by shaking a nominal loading rate with the appropriate amount of deionised water for 24 hours, followed by membrane filration. The remaining concentrations to be tested were prepared by dilution of this solution with deionised water. Since the solutions were diluted by algal pre-culture and nutrient medium, 1.25 times the nominal amount was used in preparation of the saturated solution. The technique essentially provided a water soluble fraction of nominal loading rates, further characterised by chemical analysis.
For each treatment, 240 mL of the respective test solution was prepared and mixed with 60 mL of the test culture. In this mixture, the pH-value was measured and samples for analytical determination were taken.
The test vessels were filled with ca. 40 mL of the mixture and then incubated open for 72 hours, shaken on an orbital shaker.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: Chodat
- Family: Chlorophyceae
- Order: Chlorococcales
- Source (laboratory, culture collection): The culture used on study was obtained in February 2011 by the MBM Science-bridge GmbH and were kept as a stock culture on solid agar at 8°C.
- Method of cultivation: From an aliquot of this stock on agar, the pre-culture was prepared.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- The temperature was taken as 27°C (consistent throughout the test).
- pH:
- The pH values in the control ranged from 7.7 to 8.6
- Nominal and measured concentrations:
- Nominal: Control (0), 1, 3.2, 10, 32, and 100 mg/L.
Measured (calculated from experimentally determined Zn value based on the known composition; geometric mean of 0, 24, 48 and 72 hour results): 0.26, 0.69, 2.13, 12.33 and 31.22 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass flasks
- Material, size, headspace, fill volume: 50 mL glask flasks filled to ca. 40 mL test media.
- Aeration: No
- Initial cells density: 15148 cells/mL
- Control end cells density: 751088 cells/mL
- No. of vessels per concentration (replicates): 4 replicates for each treatment group
- No. of vessels per control (replicates): 7 replicates for the control group
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium prepared and used as per OECD 201 guidance
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: Continuous
- Light intensity and quality: 4600 - 4700 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Spectrophotometer (photometric measurement of optical density (absorption).
Microscopic observation was also conducted at the end of the test to assess the appearance of the algal cells. The EC50 in relation to growth rate, AUC and yield was calculated along with the respective NOEC and LOEC values.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.25 fold - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Remarks:
- ;calculated based on measure Zn content (Zn content in test material known)
- Basis for effect:
- growth rate
- Remarks on result:
- other: 10-17 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.69 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Remarks:
- ;calculated based on measure Zn content (Zn content in test material known)
- Basis for effect:
- growth rate
- Remarks on result:
- other: not determinable
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 2.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- element
- Remarks:
- ;calculated based on measure Zn content (Zn content in test material known)
- Basis for effect:
- growth rate
- Remarks on result:
- other: not determinable
- Details on results:
- - Exponential growth in the control (for algal test): yes (validity criteria met)
- Observation of abnormalities (for algal test): Conducted microscopically
- Other: Normal growth observed at 0 to 3.2 mg/L, fewer cells observed at 10 mg/L with no cells observed at 32 to 100 mg/L.
- Any stimulation of growth found in any treatment: Yes at 1 and 3.2 mg/L (non-significant). - Results with reference substance (positive control):
- - Results with reference substance valid?
- EC50: 1.0 mg/L (95% confidence limits 0.81-1.4 mg/L), all validity criteria met and within historical range. - Reported statistics and error estimates:
- For the treatments with the nominal concentrations 3.2 and 10 mg/L, it was tested whether the differences between treatment and control were significant. In order to select a suitable test for significance, it was checked whether equality of variance was given. The calculated value is compared with an F-Test table. If teh calculated value is smaller than teh tabular value, equality of variance is given. If equality of variance is given, the t-test is used; else, the WEIR test is used.
With the t-Test, it was checked whether the differences are significant. Significance is given if the calculated t-value is bugger than the limit of significance.
Any other information on results incl. tables
Biological results on the test substance:
Parameter |
Geometric mean of measured conc. (mg/L) |
95% confidence-interval |
NOEC (growth rate) 72 hr |
0.69 |
Not determinable |
NOEC (AUC) 72 hr |
0.69 |
Not determinable |
NOEC (Yield) 72 hr |
0.69 |
Not determinable |
LOEC (growth rate) 72 hr |
2.1 |
Not determinable |
LOEC (AUC) 72 hr |
2.1 |
Not determinable |
LOEC (Yield) 72 hr |
2.1 |
Not determinable |
72 hr ErC50 |
13 |
10 – 17 mg/L |
72 hr EbC50 |
4.4 |
2.4 – 6.5 mg/L |
72 hr EyC50 |
2.4 |
Not determinable |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 value for growth rate inhibition of the test substance (ErC50) was determined to be 13 mg/L with the associated NOEC determined to be 0.69 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.