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EC number: 433-180-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-04-12 to 1999-04-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline and GLP Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- -Name of test material (as cited in study report): Reaktiv-Orange DYPR934
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentration: Test concentration 100 mg/L
- Method of administration: direct weighing (sop 2030-6600401-96 D)
Analysis of the only test concentration with and without algal inoculum) plus a control at test start and after 72 hours of exposure.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: Scenedesmus subspicatus CHODAT
- Details on test organisms:
- TEST ORGANISM
- Species: Scenedesmus subspicatus (CHODAT)
- Source (laboratory, culture collection): Institute of plant physiology, University of Göttingen
ACCLIMATION
CULTIVATION (stock cultures, precultures, test cultures)
In a light chamber at 23± 2°C and with a quantum flux which equals to 120 µE/m2xS
Water for dilution: deionized water.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- The inhibition of growth and growth rate in relation to a control is determined after 72 hours of incubation.
Test conditions
- Hardness:
- 15.4 mg CaCO3/L
- Test temperature:
- 23 ± 2°C
- pH:
- 6.8 (before application)
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- STUDY CONDITIONS: (SOP 2030-7000803-98 D)
Test procedure -Erlenmeyer flasks (300mL) with stoppers
-100 mL of test medium
-Light chamber and magnetic stirrer
-Determination of the effects on the growth of Scenedesmus subspicatus within 72 h exposure period.
COUNTING:
Microcellcounter Sysmex F-300
Digitama (SOP 2030-0001903-98 D)
Method of administration: Direct weighing (SOP 2030-0001903-96 D)
Test Concentration: 100 mg/L
Solubilizer or other auxiliaries: none
INOCULUM:
Algal suspension taken from an exponentially growing preculture which serves to adjust an initial cell density of 10000 cells/mL
CONTROL: Inoculum in nutrient medium and water for dilution.
Water for dilution: deionized water.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 68.8 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 68.8 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC0
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Details on results:
- - Exponential growth in the control (for algal test): yes
Initial cell conc.: 10 000 cells/mL
Inoculum: Algal suspension taken from an exponentially growing pre culture which serves to adjust an initial cell density of 10000 cells/ml
Control: inoculum in nutrient medium an water for dilution
Water for dilution: dionized
- EC50:68.8 mg/L
Arithmetic means of analytical values:
No toxic effects against algae at 68.8 mg/L of the organic components of the test substance (equals to 101 mg/L of the test substance including the inorganic components
-NOEC/LOEC and as EC10 and EC50 values, which means at those concentrations of the test substance that cause a 10% and a 50% reduction in either growth (Ebc) or growth rate (Erc) relative to the control.
Any other information on results incl. tables
Results-Analysis
Nominal concentr. Test substance [mg/L] |
Results by start of the test and after 72 hours [mg/L]
0 hour 72 hours |
|
Control |
< 2 |
< 2 *) |
w.algae 100 |
24 |
24 *) |
100 |
24 |
26 *) |
|
|
|
Comments: W. algae: without algae *) after filtration all values refer to TOC determination. According to the relevant product informations, 1mg/L TOC equals to 2.75 mg/L of the organic components of the test substance;
Reaktiv-Orange DYPR 934
Growth (8b) [integral of biomass]
Nominal concentration Test substance [mg/L] |
Growth [integral of biomass] |
% inhibition [+] or % increase [-] of growth (control = 0 %) |
Control |
588000 |
0,0 |
100 |
595000 |
-1.2 |
|
|
|
|
|
|
Growth rate (r)
Nominal concentration. Test substance [mg/L] |
Growth rate [1/d] |
% inhibition [+] or % increase [-] of growth (control = 0 %) |
Control |
1.3 |
0.0 |
100 |
1.3 |
0.0 |
|
|
|
|
|
Comment relevant to result:
Purity of the test substance: see chapter characterization of the test material.
Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
The test result refers to a concentration of Reaktiv Orange DYPR 934 which was calculated directly from analytically determined TOC values. According to the relevant substance informations, 1 mg/L TOC equals to 2.75 mg/L of the organic components of the organic test substance. As these organic correspond to 68 % of the molecular weight of the test substance, 100 mg/L of the test substance equals to 24.7 mg/L of organic C. Thus the analytical values indicate a very good recovery rate.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Arithmetic means of analytical values:
No toxic effects against algae at 68.8 mg/l of the organic components of the test substance (equals to 101 mg/L of the test substance including the inorganic components) - Executive summary:
In a 72 hours "algal growth inhibition test", exponetially-growing cultures of a Scenedesmus subspicatus CHODAT were exposed to various concentrations of the test substance over several generations under defined conditions.
During the incubation period, during the cell density was measured every 24 hours. The inhibition of growth and growth rate in relation to a control is determined after 72 hours of incubation.
The results are expressed as NOEC/LOEC and as EC10 and EC50 values, which means at those concentrations of the test substance that cause a 10 % and a 50 % reduction in either growth (Ebc) or growth rate (Erc) relative to the control.
No toxic effects against algae at 68.8 mg/l of the organic components of the test substance (equals to 101 mg/L of the test substance including the inorganic components) were observed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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