Registration Dossier
Registration Dossier
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EC number: 700-291-0 | CAS number: 1000701-92-7
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-hexyloxyethanol
- EC Number:
- 203-951-1
- EC Name:
- 2-hexyloxyethanol
- Cas Number:
- 112-25-4
- Molecular formula:
- C8H18O2
- IUPAC Name:
- 2-(hexyloxy)ethanol
- Details on test material:
- - Name of test material (as cited in study report): N-Hexylglykol
- Analytical purity: 98.23%
- Purity test date: 27 Jun 1991
- Substance No.: 91/238
- Lot/batch No.: Tank B 773
- Physical state: liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH, Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD): about 4 month
- Length at study initiation (length definition, mean, range and SD): 2.9 cm (range: 2.7 - 3.1)
- Weight at study initiation (mean and range, SD): 0.233 g (range 0.17 - 0.30)
- Feeding during test
- Food type: "Tetra Min" standard feed for aquarium fish Tetra - Werke, Melle, Germany
- Amount: ad libitum
- Frequency: 1 day before and during exposure
ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- about 2.5 mmol/L (about 250 mg/l CaCO3)
- Test temperature:
- 23 °C
- pH:
- about 8.0 - 8.6
- Dissolved oxygen:
- 7.7 - 8.2
- Nominal and measured concentrations:
- Nominal concentrations: 0, 46.4, 100.0, 215.0, 464.0 and 1000.0 mg/L
Analytical concentrations after 0 h: 0, 39.5, 88.0, 206.0, 469.0 and 1026.0 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Type: open
- Material, size, fill volume: glass, 30 cm x 22 cm x 24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- Biomass loading rate: 0.23
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water of the city of Frankenthal, not chlorinated and passed through an active carbon filter, aerated
OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 day-night regime
EFFECT PARAMETERS MEASURED:
mortality and symptoms of intoxication
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study
- Results used to determine the conditions for the definitive study: LC 50 after 96 h: between 100 and 1000 mg/L
Preparation of the test solutions:
Separately for each concentration test substance were added to the test water. Subsequently the fish were placed into the aquaria within 25 min after the test material was added.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 - 220 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 215 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Cumulative mortality after x hours
Nominal concentration (mg/L) 1 4 24 48 72 96 0 0 0 0 0 0 0 46.4 0 0 0 0 0 0 100.0 0 0 0 0 0 0 215.0 10 10 10 10 10 10 464.0 10 10 10 10 10 10 1000.0 10 10 10 10 10 10 Symptoms of intoxication after x hours
Nominal concentration (mg/L) 1 4 24 48 72 96 0 - - - - - - 46.4 - - - - - - 100.0 A A (A) (A) (A) (A)
Applicant's summary and conclusion
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