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EC number: 403-830-5 | CAS number: 89331-94-2 B 290; BK 400; CK 34; DIBUTYL-N-102; DX-20; FAT NR. 40391/A; FLUORAN BLACK BD 869; FLUORAN SCHWARZ BD 869; NOIR FLUORANE BD 869; ODB-2; PSD-290; SENOR-2; TG-31; TH-108; WINCON-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 August to 08 September 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study undertaken at GLP accredited laboratory to internationally accepted guidelines.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.6 (Degradation: Chemical Oxygen Demand)
- Deviations:
- not specified
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge:The aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage.
- Laboratory culture:
- Method of cultivation: Standard nutrient medium added
- Storage conditions: 280 ml BOD bottles (darkened glass) with ground glass stoppers.
- Storage length: 0, 5, 15 and 28 days
- Preparation of inoculum for exposure:
- Pretreatment: incubated as 21 ± 1°C
- Concentration of sludge:
- Initial cell/biomass concentration:
- Water filtered: yes
- Type and size of filter used, if any: Whatman GFA filter paper - Duration of test (contact time):
- 0 - 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 5
- Sampling time:
- 28 d
- Details on results:
- The test substance attained 5% biodegradation after" 28 days and cannot, therefore be considered as readily biodegradable under the strict terms of Annex V.C6.
Sodium benzoate attained 89% degradation within 28 days. Oxygen depletions in the inoculated and non-inoculated control series were within the prescribed limits. - Validity criteria fulfilled:
- not specified
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance attained 5% biodegradation after 28 days and cannot, therefore be considered as readily biodegradable under the strict terms of Annex V.C6.
Sodium benzoate attained 89% degradation within 28 days. - Endpoint:
- biodegradation in water: screening tests
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 16 April to 14 May 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study undertaken at GLP accredited laboratory to internationally accepted guidelines.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.5 (Degradation: Biochemical Oxygen Demand)
- Deviations:
- not specified
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: The aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage.
- Laboratory culture:
- Method of cultivation:
- Storage conditions: 5 litre glass culture vessels (containing 3 litres of culture) fitted with gas-tight lids, sintered glass air stones and magnetic stirrers.
- Storage length:
- Preparation of inoculum for exposure:
- Pretreatment: The sample was aerated for 6 days at test temperature prior to use. This was to facilitate the degradation of background dissolved organic carbon in the sample and thereby, reduce the carryover into the test vessels. Immediately prior to use, the suspended solids content was determined and adjusted using nutrient media.
- Concentration of sludge: 500 mg/l
- Initial cell/biomass concentration:
- Water filtered: yes
- Type and size of filter used, if any: - Duration of test (contact time):
- 0 - 28 d
- Initial conc.:
- 40 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 28 d
- Results with reference substance:
- The standard substance, sodium benzoate, attained 61% biodegradation within 28 days, thereby confirming the suitability of the inoculum and the culture conditions.
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- ODB-2 attained only 1 - 2% biodegradation at 40 mg/l after 28 days and cannot, therefore, be termed as inherently biodegradable.
Referenceopen allclose all
Description of key information
In study 29(a), the test substance attained 5% biodegradation after 28 days and cannot, therefore be considered as readily biodegradable under the strict terms of Annex V.C6.
In study 29(b), ODB-2 attained only 1 - 2% biodegradation at 40 mg/l after 28 days and cannot, therefore, be termed as inherently biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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