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EC number: 273-601-0 | CAS number: 68990-47-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Fatty acids, tall-oil, reaction products with diethylenetriamine, maleic anhydride, tetraethylenepentamine and triethylenetetramine
- EC Number:
- 273-601-0
- EC Name:
- Fatty acids, tall-oil, reaction products with diethylenetriamine, maleic anhydride, tetraethylenepentamine and triethylenetetramine
- Cas Number:
- 68990-47-6
- Molecular formula:
- The substance is a UVCB substance. One of the most likely and the smallest molecule arising from the reaction process is assumed to be: C18H33O. C18H31O. 2C4H12N3 . C4H2O2
- IUPAC Name:
- Reaction product of 2,5-Furandione with reaction products of tall-oil fatty acids, diethylenetriamine, triethylenetetramine and tetraethylenepentamine
- Details on test material:
- Test item name: Fatty acid, tall-oil, reaction products with diethylenetriamine, maleic anhydride, tetraethylenepentamine and triethylenetetramine
Batch number : TEE3316/22
Molecular weight: > 700
Declared purity: 97 %
ChemService sample code: 2901348-001
Storage conditions: Room temperature/dark conditions
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Juvenile fish were used and all of them were approximately the same age and size. In accordance with the test guidelines, the fish were held in the ChemService laboratories for more than 12 days at the same conditions regarding water quality, temperature and illumination, as during the test. During the holding period (21 days), the fish were fed with commercial fish food (Tetramin Granules) until 48 hours before the test initiation.
The used batch of fishes was accepted since no mortality and no signs of distress were observed during the acclimation period.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 10 - 250 mg/L as CaCO3
- Test temperature:
- The measured values ranged between 20.4 and 23.9 °C.
- pH:
- 7.14 – 7.34
- Dissolved oxygen:
- During the test period, the dissolved oxygen concentrations in the test medium and the control were always above 60 per cent of the air saturation value .
- Details on test conditions:
- Photoperiod: 16 hours light and 8 hours darkness daily ; light intensity was 50 - 500 lux, with 2 daily 30-minutes transition periods
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
A limit test under static conditions was performed to evaluate the effect of the test item on zebra fish (Danio rerio).
The fishes were checked for mortality and abnormalities in appearance and behaviour after 2, 24, 48, 72 and 96 hours.
In the control 0% mortality was observed during the test period. This value complies with the validity criteria of the test, according to the mentioned guidelines, that provide a maximum mortality of fishes exposed of 10% in the control medium at the end of the test.
The observed effects in the test concentration and in the control are reported in Table 1.
Table 1
Lethal effect of Fatty acid, tall-oil, reaction products with diethylenetriamine, maleic anhydride, tetraethylenepentamine to zebra fish.
Nominal
test item concentration
[mg/L]
Replicate
n. of exposed
fish
n. dead fish
2 h
24 h
48 h
72 h
96 h
0.0
(Negative control)
A
10
0
0
0
0
0
0.0
(Negative control)
B
10
0
0
0
0
0
100.0
A
10
0
0
0
0
0
100.0
B
10
0
0
1
1
1
The NOEC and the LOEC were determined directly from the Raw Data by statistical comparison with the control (Fisher’s Exact test) at each observation time.
The NOEC (highest concentration tested without toxic effects after the exposure period) of test item to zebra fish was determined to be equal to the tested concentration, 100.0 mg/L.
Based on the observed effects, the LOEC and the 96-h LC50were determined to be greater than 100.0 mg/L.
As for the test medium appearance, no remarkable observation was made. The test medium was a clear solution throughout the whole test duration.
Light intensity during the test was measured at test initiation, after 48 and 96 hours and it was in the range 208 – 272 Lux, according to the mentioned guidelines (50 – 500 Lux).
The range of temperature in the control tanks was between 20.8 –with a mean value ofand a standard deviation of 0.9; while the temperature values in the test item solutions ranged between 20.4 andwith a mean value ofand a standard deviation of 0.8. The temperature values measured were slightly below the recommended range, however this deviation did not affect the outcome of the study.
During the test period, the dissolved oxygen concentrations in the test medium and the control were always above 60 per cent of the air saturation value as showed in Table 2.
The pH values were in the range 7.03 - 7.45 as required by the mentioned guidelines and did not vary more than 0.11 units (Table 2) between the start and the end of test period, thus meeting the validity criteria for pH (maximum difference = 1.5 pH unit).
Table 2.
Dissolved oxygen concentrations and pH values in the test media at test initiation and during the exposure time.
Nominal test item concentration (mg/L)
(Negative control)
A
(Negative control)
B
100.0
A
100.0
B
Dissolved
oxygen
(%)
0 h
64.5
63.8
63.3
64.9
24 h
77.7
77.1
70.9
72.6
48 h
73.4
72.6
70.2
74.5
72 h
74.5
73.8
72.7
73.1
96 h
76.7
73.7
73.3
71.9
pH
0 h
7.14
7.19
7.28
7.34
24 h
7.00
7.13
7.24
7.28
48 h
7.31
7.42
7.45
7.42
72 h
7.28
7.32
7.38
7.36
96 h
7.03
7.23
7.30
7.32
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item “Fatty acid, tall-oil, reaction products with diethylenetriamine, maleic anhydride, tetraethylenepentamine” caused 0.5% mortality to zebra fish after 96-hours of exposure at the nominal product concentration of 100 mg/L in a static test.
The NOEC, LOEC and LC50 values after 96 h, calculated based on the nominal concentration, were assessed to be as follows:
96 h NOEC: 100.0 mg/L
96 h LOEC: > 100.0 mg/L
96 h LC50: > 100.0 mg/L
96 h LC50: > 100.0 mg/L - Executive summary:
The acute toxicity of the test item Fatty acid, tall-oil, reaction products with diethylenetriamine, maleic anhydride, tetraethylenepentamine and triethylenetetramine to zebra fish (Danio rerio)was determined in a limit, 96‑hour static test according to:
- OECD Guideline for Testing of Chemicals, N. 203. “Fish, acute toxicity test”, 1992
- OPPTS 850.1075 guideline “Fish acute toxicity test, freshwater and marine”, 1996.
For this purpose, juvenile fish of the speciesDanio reriowere exposed, after an acclimation period (21 days), to an aqueous test medium containing the test item at the only nominal concentration of 100.0 mg/L of product. Two replicate for the test concentration and for negative control were prepared.
The test organisms were checked for mortality after 2, 24, 48, 72 and 96 hours from test initiation.
The pH values of the test medium after preparation was in the range 7.28 – 7.34 for test item and in the range 7.14 – 7.19 for negative control. Therefore, the pH of the test medium was not adjusted before the start of the test.
The dissolved oxygen was all the time above 60 per cent of the air saturation value both in each replicate of negative control and test solution.
The temperature during the test was recorded by the mean of PC system and it was in the range 20.7 -for controls replicate and 20.4 -for the test item solutions. The temperature was slightly below the OECD’s recommended range (23.0±2.0 °C), however it did not affect the results of the study.
Light intensity during the test was measured at test initiation, after 48 and 96 hours and it was in the range 208 – 272 Lux according to the mentioned guidelines that provided a range of light intensity of 50 – 500 Lux.
In the control 0% mortality was observed during the test period. This value complies with the validity criteria of the test, according to the mentioned guidelines, that provide a maximum mortality of fishes exposed of 10% in the control medium at the end of the test.
In the test item solution one fish was found to be dead at the end of the test period, therefore the NOEC (highest concentration tested without toxic effects after the exposure period of 96 hours) of Fatty acid, tall-oil, reaction products with diethylenetriamine, maleic anhydride, tetraethylenepentamine and triethylenetetramine to zebra fish was determined to be equal to the tested concentration (nominal 100 mg/L).
Based on the observed effects, the LOEC and the 96-h LC50were determined to be greater than the 100.0 mg/L as product.
During the test period any behaviour and/or pathological signs on exposed fishes were observed.
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