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Diss Factsheets
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EC number: 210-323-0 | CAS number: 612-83-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
Additional information
Data on toxicokinetics were reported from secondary sources. However, often no clear distinction was made whether the testing was performed with the submission subtance (3,3'-dichlorobenzidine dihydrochloride) or the free base (3,3'-dichlorobenzidine). In the following it is assumed, that the findings for the free base also apply to the submission substance.
Reliable data on the toxicokinetics of the submission substance in humans are missing. Based on animal data, mainly in the rat, it can be concluded that the submission substance is absorbed after oral and dermal exposure. After oral exposure absorption is nearly complete. Dermal exposure for 24 hours to the base resulted in about 50% absorption which can also be assumed for the submission substance. Information on absorption in animals following inhalative exposure are missing.
The submission substance is rapidly distributed throughout the body and there is evidence that the test item can cross the placental barrier. There is no information whether it can be transferred to the breast milk.
N-acetylation seems to be the major pathway for metabolism of the submission substance in mammals. The metabolites N,N'-diacetyl-3,3'dichlorobenzidine and N-acetyl-3,3'-dichlorobenzidine have been identified in the urine. Additionally, there is indirect evidence for the formation of N-oxidation, nitroso-formation in the rat. Glucuronidation has been described.
The submission substance and its metabolites are mainly excreted via feces and partially via urine. Mainly unconjugated metabolites are excreted via urine whereas most of the material found in bile and feces consisted of conjugated metabolites. The submission substance seems to be subject to enterohepatic recirculation. Biphasic elimination has been described for plasma, liver, kidneys and lungs of rats. The half lives in plasma were 6 hours and 14 hours for the rapid and slow phase. For the individual organs even longer half-lives were reported.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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