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Diss Factsheets
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EC number: 500-036-1 | CAS number: 25214-70-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: A GLP study carried out in accordance with an OECD Guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 4,4'-methylenedianiline
- EC Number:
- 202-974-4
- EC Name:
- 4,4'-methylenedianiline
- Cas Number:
- 101-77-9
- Molecular formula:
- C13H14N2
- IUPAC Name:
- 4,4'-methylenedianiline
- Details on test material:
- 4,4'-methylenedianiline (4,4'-MDA)
Uniformly [14C] ring labeled
Lot no CFQ8060, Amersham Life Science, Piscataway, New Jersey, USA.
Specific activity: 2.42 +/- 0.00 mCi/mmole (mean +/-1 std. Dev., n=3)
Radiochemical purity: 96.0 +/- 0.7% (mean +/- 1std. Dev., n=3)
Approx. chemical purity: 94.9% based on peak area percentage from HPLC-UV analysis.
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Municipal activated sludge from the wastewater treatment plant Mannheim/Baden Württemberg (Germany) was collected on 06 July 2007 from the aeration tank of the plant and aerated in the laboratory until further use. A suitable aliquot of the activated sludge suspension was sieved using a finely woven mesh with a mesh size of about 1 mm. After settling, the supernatant was discarded and the sludge suspension was filled up with tap water. This washing procedure was repeated three times. The suspended solids concentration of the resulting sludge suspension will be determined, and a suitable portion was used to inoculate the mineral medium at a final suspended solids concentration of 30 mg/L. The inoculum was pre-adapted to the test conditions by stirring and aeration with CO2-free air at the test temperature for about five days. After this adaptation period, the inoculated mineral medium (1.5 L) was dispensed into the various test vessels.
Reason for the choice of the biological test system: The test guideline recommends the usage of activated sludge from a wastewater treatment plant, treating municipal sewage. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 500 µg/L
- Based on:
- other: corresponding to a total radioactivity of about 11 microCi in the test assay.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- radiochem. meas.
- Details on study design:
- Test temperature 22+/-2 degree C
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 46
- St. dev.:
- 3.96
- Sampling time:
- 28 d
- Details on results:
- 46% biodegradation after exposure of 28 days
53% (standard deviation +/-3.54%) after exposure of 63 days.
BOD5 / COD results
- Results with reference substance:
- 66% degradation after 14 days (%CO2/ThCO2)
Any other information on results incl. tables
The test substance was partly or moderately biodegradable. The study was based on OECD Guideline 301B and measured the evolution of CO2 to determine the degree of biodegradation. Therefore, no BOD was determined.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: moderately biodegradable
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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