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EC number: 208-754-4 | CAS number: 540-72-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 25 April 1988 - 6 May 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to GLP and OECD471. Formally there is a deficiency due to lack of strains for capable of detecting cetain oxidising or cross-linking agents. This is not suspected to be of impact to thiocyantes.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- With this test it is not possible to identify certain oxidising mutagens, cross-linking agents and hydrazines. Such substances may be detected by E.coli WP2 strains or S. typhimurium TA102 which have an AT base pair at the primary reversion site in stead of GC base pairs which the strains tested in this study have. No independent repeat was performed.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Details on test material:
- Identification: Ammoniumthiocyanate
Chemical Name: Ammoniumthiocyanate. CAS No. 1762-95-4
Physical appearance: white, crystalline powder
Purity: chemically pure (information of the sponsor)
Solubility: water
Storage: Refrigerator, 4 °C
No further details.
Constituent 1
Method
- Target gene:
- the histidine operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: see: "any other information....."
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- other: see: "any other information....."
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix
- Test concentrations with justification for top dose:
- 50, 100, 500, 1000, 2500 and 5000 µg/plate
- Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: see: "any other information....."
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: not applicable
- Exposure duration: 48 hours
- Expression time (cells in growth medium): 48 hours
- Selection time (if incubation with a selection agent): not applicable
- Fixation time (start of exposure up to fixation or harvest of cells): not applicable
SELECTION AGENT (mutation assays): histidine
NUMBER OF REPLICATIONS: triplicate
NUMBER OF CELLS EVALUATED: The reverted colonies were counted automatically with an Artec Colony Counter (M 880).
DETERMINATION OF CYTOTOXICITY
- Method: number of colonies - Evaluation criteria:
- Not stated in the report. Performed according to OECD471
There are several criteria for determining a positive result, such as a concentration-related increase over the range tested and/or a reproducible increase at one or more concentrations in the number of revertant colonies per plate in at least one strain with or without metabolic activation system.
Biological relevance of the results should be considered first. Statistical methods may be used as an aid in evaluating the test results. However, statistical significance should not be the only determining factor for a positive response. A test substance for which the results do not meet the above criteria is considered nonmutagenic in this test. - Statistics:
- Not stated in the report.
Results and discussion
Test results
- Species / strain:
- other: S. typhimutium TA98, TA100, TA1535, TA1538 and TA1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: no data
- Effects of osmolality: no data
- Evaporation from medium: no data
- Water solubility: water us the vehicle
- Precipitation: not observed
- Other confounding effects: no data
RANGE-FINDING/SCREENING STUDIES: no data
COMPARISON WITH HISTORICAL CONTROL DATA: no data
ADDITIONAL INFORMATION ON CYTOTOXICITY: no data
The tester strains,are checked at regular intervals for their genetic markers, according to the methods of Ames et al. ( 4 ) :
1. Histidine-requirement
2. rfa-mutation: crystall violet sensitivity
3. uvrB-mutation: UV-sensitivity
4. R-factor: ampicillin resistance of the strains TA98 and TA100
All strains showed the expected criteria. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Mutation test
strain |
Dose level (µg/plate) |
Metabolic activation |
Mean revertant colony counts |
SD |
TA98 |
0 |
- |
22 |
4 |
50 |
- |
18 |
4 |
|
100 |
- |
21 |
5 |
|
500 |
- |
17 |
1 |
|
1000 |
- |
24 |
2 |
|
2500 |
- |
23 |
6 |
|
5000 |
- |
27 |
4 |
|
0 |
+ |
25 |
3 |
|
50 |
+ |
22 |
5 |
|
100 |
+ |
21 |
2 |
|
500 |
+ |
24 |
2 |
|
1000 |
+ |
33 |
4 |
|
2500 |
+ |
26 |
2 |
|
5000 |
+ |
24 |
1 |
|
TA100 |
0 |
- |
128 |
18 |
50 |
- |
140 |
6 |
|
100 |
- |
131 |
5 |
|
500 |
- |
151 |
11 |
|
1000 |
- |
143 |
15 |
|
2500 |
- |
133 |
10 |
|
5000 |
- |
125 |
11 |
|
0 |
+ |
92 |
10 |
|
50 |
+ |
117 |
8 |
|
100 |
+ |
116 |
2 |
|
500 |
+ |
143 |
7 |
|
1000 |
+ |
147 |
15 |
|
2500 |
+ |
169 |
22 |
|
5000 |
+ |
144 |
5 |
|
TA1535 |
0 |
- |
21 |
10 |
50 |
- |
13 |
3 |
|
100 |
- |
17 |
7 |
|
500 |
- |
21 |
5 |
|
1000 |
- |
18 |
4 |
|
2500 |
- |
18 |
5 |
|
5000 |
- |
20 |
2 |
|
0 |
+ |
14 |
6 |
|
50 |
+ |
14 |
3 |
|
100 |
+ |
11 |
5 |
|
500 |
+ |
15 |
3 |
|
1000 |
+ |
18 |
5 |
|
2500 |
+ |
16 |
6 |
|
5000 |
+ |
15 |
2 |
|
TA1537 |
0 |
- |
7 |
3 |
50 |
- |
7 |
4 |
|
100 |
- |
8 |
2 |
|
500 |
- |
8 |
5 |
|
1000 |
- |
7 |
2 |
|
2500 |
- |
10 |
2 |
|
5000 |
- |
8 |
2 |
|
0 |
+ |
8 |
2 |
|
50 |
+ |
8 |
2 |
|
100 |
+ |
10 |
2 |
|
500 |
+ |
5 |
1 |
|
1000 |
+ |
6 |
3 |
|
2500 |
+ |
9 |
2 |
|
5000 |
+ |
6 |
2 |
|
TA1538 |
0 |
- |
8 |
2 |
50 |
- |
10 |
2 |
|
100 |
- |
10 |
3 |
|
500 |
- |
13 |
3 |
|
1000 |
- |
14 |
4 |
|
2500 |
- |
10 |
2 |
|
5000 |
- |
12 |
4 |
|
0 |
+ |
13 |
2 |
|
50 |
+ |
11 |
4 |
|
100 |
+ |
8 |
2 |
|
500 |
+ |
9 |
4 |
|
1000 |
+ |
8 |
2 |
|
2500 |
+ |
8 |
3 |
|
5000 |
+ |
10 |
1 |
- Absence
+ Presence
SD Standard deviation
Positive control Mutation test 1:
Strain |
Compound |
Dose level (µg/plate) |
Metabolic activation |
Mean revertant colony counts |
SD |
TA98 |
2-nitrofluorene/2-aminoanthracene |
5/1 |
- |
296/21 |
61/2 |
TA100 |
2-nitrofluorene/2-aminoanthracene |
5/1 |
- |
628/153 |
22/23 |
TA1535 |
Sodium azide/2-aminoanthracene |
1/1 |
- |
282/15 |
13/7 |
TA1537 |
9-aminoacridine/2-aminoanthracene |
50/1 |
- |
104/10 |
11/3 |
TA1538 |
2-nitrofluorene/2-aminoanthracene |
5/1 |
- |
66/11 |
1/5 |
TA98 |
2-nitrofluorene/2-aminoanthracene |
5/1 |
- |
0/1021 |
0/139 |
TA100 |
2-nitrofluorene/2-aminoanthracene |
5/1 |
+ |
0/1547 |
0/186 |
TA1535 |
Sodium azide/2-aminoanthracene |
1/1 |
+ |
0/154 |
0/10 |
TA1537 |
9-aminoacridine/2-aminoanthracene |
50/1 |
+ |
0/120 |
0/9 |
TA1538 |
2-nitrofluorene/2-aminoanthracene |
5/1 |
+ |
0/508 |
0/37 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the conditions of this Salmonella/Microsome assay the test material Ammoniumthiocyanate has no mutagenic effects. - Executive summary:
The test material Ammoniumthiocyanate was tested according to GLP and OECD471 in the Salmonella/ Microsome plate incorporation assay in the strains TA 98, TA 100, TA 1535, TA 1537 and TA 1538 in the absence and presence of metabolic activation. Under the conditions of this Salmonella/Microsome assay the test material Ammoniumthiocyanate has no mutagenic effects.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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