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EC number: 800-984-9 | CAS number: 1428547-35-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Media preparation
The test medium used was ASTM medium. It was reconstituted with chemical reagents and deionised water. The test medium was performed several times a week.
Test solution preparation
Since the test item is poorly soluble under the test conditions, a saturated solution of test item (limit of solubility LS) was prepared. A solution with a loading rate of approximately 1g/L was prepared and stirred with an agitator for several days. Then, this stock solution was filtered with cellulose acetate filters at 0.45 µm. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Fertilised eggs of Danio rerio produced from the laboratory genitors were used as soon as possible after fertilisation. At the laboratory, male and female genitors were separated in aquariums with a continuous renewal of the water.
The day before the experiment, male and females were added in the nest box. Egg laying and fertilisation occurred at « dawn », simulated with lighting. Eggs were harvested and a selection was done under microscope in order to select fertilised eggs according to Annex 3 of OECD 236 guideline.
Breeding conditions for genitors were the following:
Medium: water from the water system (mix of tapwater and deionized water)
Temperature: 23 °C ± 2
Light/dark cycle: 16 h/8 h
Dissolved oxygen concentration: = 80 % of the air saturation value
Feeding: twice a day ad libidum (once with small granular and once with freezing food (chironomus, artemia) - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 34 d
- Post exposure observation period:
- Exposure took place on embryo as well as for 30 days post-hatch.
- Test temperature:
- 26.1 - 27.1°C (min - max)
- pH:
- 7.2 - 7.8 (min-max)
- Dissolved oxygen:
- 69.8 - 103.0% (min-max)
- Nominal and measured concentrations:
- Nominal: Five concentrations at LS/81 – LS/27 – LS/9 – LS/3 and LS were prepared in ASTM medium. A control was set up with the test medium only. LS = limit of water solubility (i.e. saturated solu33tion)
Measured: see table 1. - Details on test conditions:
- Test containers
The test was performed in glass crystallizer of 50 mL, filled to 25 mL during the five first days, in glass crystallizer of 400 mL filled to 125 mL between days 5 and days 14, in glass aquarium of 1.5L filled to 250 mL between days 14 and days 21 and filled to 500 mL until the end of the test. Fish loading rate was less than 5 g.L-1. The maximal fish weight at the end of the test was 0.2666g per aquarium for a volume of 500 mL.
The test was performed under semi-static conditions (renewal three times a week). The test organisms were retained in the test vessels with the exception of days of change of test containers whilst a proportion (at least two thirds) of the test solution / control volume was changed.
Performing the ecotoxicity test
Five concentrations at LS/81 – LS/27 – LS/9 – LS/3 and LS were prepared in ASTM medium. A control was set up with the test medium only. There were 4 replicates for each treatment with 20 eggs per replicate. The test was performed in an experimental room maintained at 26° C ± 1.5 with a day / night cycle of 16h / 8h. pH and oxygen of test solutions were recorded before and after renewal. Before hatching, the number of coagulated eggs was noted, and after hatching, the number of deaths in each replicate was recorded daily. The test was performed during 30 days post hatching. At the end of the test, fish were measured individually and then weighed in batches. Means of lengths and weights were calculated by replicate. Two days post hatching, fish were fed with Nobilfluid ® and live rotifera (Brachionus plicatilis) twice or three times a day. Six days post hatching, Nobilfluid ® was stopped and live artemia (Artemia salina) were added.
Euthanasia
Fish were euthanized in order to carry out the measurements of weight and length. For this, fish were placed for about two minutes in a crystallizer containing sodium methanesulfonate or MS222 (CAS number 886-86-2) at a lethal concentration of 300 mg/L. - Reference substance (positive control):
- no
- Duration:
- 34 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 81.2 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Key result
- Duration:
- 34 d
- Dose descriptor:
- EC10
- Effect conc.:
- 5.75 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 34 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.67 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- weight
- Remarks:
- dry weight
- Details on results:
- See Table 1 to 15.
- Reported statistics and error estimates:
- The hatching, mortality and abnormality percentage were determined for each concentration. EC10 (Effect Concentration for 10% effect), EC50 and confidence limits were obtained by calculation according to Regtox® Macro (“Vindimian”), using the Hill model.
The equation of Hill (1910) was described to model the binding of oxygen to haemoglobin. It was shown to be relevant for many other mechanisms like binding of a ligand to a receptor, Michaelian or non Michaelian enzymatic kinetics, dose or concentration response in toxicology and ecotoxicology (Vindimian 1983, Garric 1990). The logistic model is also used which is similar to the Hill model.
The model used in this macro is written in the form proposed by Duggleby in 1981. It fits different cases where the measurement of a biological parameter is plotted versus concentration or dose. Two parameters: Hill number and EC50 are characteristics of the probability function written as follows:
f(x) = 1 / [ 1 + (EC50 / x)^(nh)]
It should be noted that f(x)=1/2 when x = EC50.
The fitting is based on the algorithm of Marquardt (1963) which is robust and fast. It needs initial estimates of the parameters and optimize them by successive iterations.
The confidence intervals on the parameters are estimated by a bootstrap simulation which is entirely non parametric and is considered to be well adapted to non linear models (Efron 1990).
The NOEC (No Observed Effect Concentration) were determined by statistical test from replicate data on hatching, mortality and on length and weight. Statistical calculations were made using R statistical software. - Validity criteria fulfilled:
- yes
- Conclusions:
- 34d-EC10 was 5.75 µg/L for larval survival.
- Executive summary:
Long-term toxicity of the registered substance to fish was evaluated in a GLP study according to OECD 210 TG. Embryo and larvae were exposed to graded concentrations of the test item for 34 days in quadruplicate of 20 eggs each under semi-static conditions (control ; limit of solubility LS ; LS/3 ; LS/9 ; LS/27 ; LS/81). Solutions were renewed 3 times a week. Actual concentrations were monitored on a regular basis. Only one larvae hatched at LS and died the following day. All larvae died in the LS/27 treatment. NOEC/EC10 were determined for hatching rate, survival rate and growth (dry weight). The lowest of the three was EC10 for survival rate, which was 5.75 µg/L.
Reference
Table 1: Test concentrations expressed as geometrical means
|
Test item geometrical mean (µg/L) |
|
|
After hatching |
End of the test |
LS/81 |
5.11 |
4.38 |
LS/27 |
9.41 |
6.67 |
LS/9 |
22.07 |
12.13 |
LS/3 |
81.20 |
34.12 |
LS |
651.79 |
651.79 |
Control | <LoD | < LoD |
Table 2: Hatching and survival rates.
Replicate |
Number of hatched larvae on D5 |
Hatching rate per replicate (%) |
Mean hatching rate (%) |
Number of survivors at the end of the test |
Survival rate per replicate (%) |
Mean survival rate (%) |
||
Control |
R1 |
19 |
95.00 |
93.8 |
15 |
78.9 |
76.0 |
|
R2 |
20 |
100.00 |
15 |
75.0 |
||||
R3 |
17 |
85.00 |
13 |
76.5 |
||||
R4 |
19 |
95.00 |
14 |
73.7 |
||||
LS/81 |
R1 |
20 |
100.00 |
97.5 |
15 |
75.0 |
75.7 |
|
R2 |
18 |
90.00 |
14 |
77.8 |
||||
R3 |
20 |
100.00 |
14 |
70.0 |
||||
R4 |
20 |
100.00 |
16 |
80.0 |
||||
LS/27 |
R1 |
17 |
85.00 |
93.8 |
4 |
23.5 |
21.6 |
|
R2 |
20 |
100.00 |
3 |
15.0 |
||||
R3 |
18 |
90.00 |
5 |
27.8 |
||||
R4 |
20 |
100.00 |
4 |
20.0 |
||||
LS/9 |
R1 |
20 |
100.00 |
96.3 |
2 |
10.0 |
3.9 |
|
R2 |
19 |
95.00 |
0 |
0.0 |
||||
R3 |
20 |
100.00 |
0 |
0.0 |
||||
R4 |
18 |
90.00 |
1 |
5.6 |
||||
LS/3 |
R1 |
20 |
100.00 |
100.0 |
0 |
0.0 |
0.0 |
|
R2 |
20 |
100.00 |
0 |
0.0 |
||||
R3 |
20 |
100.00 |
0 |
0.0 |
||||
R4 |
20 |
100.00 |
0 |
0.0 |
||||
LS |
R1 |
1 |
5.00 |
1.3 |
0 |
0 |
0 |
|
R2 |
0 |
0.00 |
- |
0 |
||||
R3 |
0 |
0.00 |
- |
0 |
||||
R4 |
0 |
0.00 |
- |
0 |
Table 3: Weight and length of surviving larvae at the end of the test
Replicate |
Length (mm) |
Average length (mm) |
Wet weight (g) |
Average wet weight (g) |
Dry weight (g) |
Average dry weight (g) |
||
C01 |
R1 |
12.96 |
13.31 |
17.77 |
17.25 |
3.15 |
3.20 |
|
R2 |
13.27 |
14.94 |
2.99 |
|||||
R3 |
13.55 |
17.55 |
3.60 |
|||||
R4 |
13.45 |
18.75 |
3.06 |
|||||
C02 |
R1 |
13.34 |
13.08 |
17.77 |
16.54 |
3.24 |
3.29 |
|
R2 |
13.28 |
17.57 |
3.71 |
|||||
R3 |
12.60 |
14.86 |
3.24 |
|||||
R4 |
13.10 |
15.96 |
2.97 |
|||||
C03 |
R1 |
10.51 |
10.51 |
10.48 |
10.13 |
2.18 |
1.98 |
|
R2 |
12.03 |
14.20 |
2.97 |
|||||
R3 |
10.85 |
11.68 |
2.20 |
|||||
R4 |
8.64 |
4.15 |
0.57 |
|||||
C04 |
R1 |
9.16 |
9.74 |
5.20 |
6.10 |
0.40 |
0.45 |
|
R4 |
10.32 |
7.00 |
0.50 |
Table 4: Number of dead eggs per day and cumulative hatch per replicate in the control group
|
Control group |
|||||||
Date |
R1 |
R2 |
R3 |
R4 |
||||
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
|
D1 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
0 |
D2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
D3 |
0 |
2 |
0 |
5 |
0 |
3 |
0 |
4 |
D4 |
0 |
19 |
0 |
20 |
0 |
17 |
0 |
19 |
D5 |
1 |
19 |
0 |
20 |
2 |
17 |
1 |
19 |
Table 5: Number of dead eggs per day and cumulative hatch per replicate in the LS/81 group
|
LS/81 |
|||||||
Date |
R1 |
R2 |
R3 |
R4 |
||||
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
|
D1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
20 |
D2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
20 |
D3 |
0 |
5 |
0 |
3 |
0 |
6 |
0 |
20 |
D4 |
0 |
20 |
0 |
18 |
0 |
20 |
0 |
20 |
D5 |
0 |
20 |
1 |
18 |
0 |
20 |
0 |
20 |
Table 6: Number of dead eggs per day and cumulative hatch per replicate in the LS/27 group
|
LS/27 |
|||||||
Date |
R1 |
R2 |
R3 |
R4 |
||||
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
|
D1 |
0 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
D2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
D3 |
0 |
2 |
0 |
3 |
0 |
5 |
0 |
6 |
D4 |
0 |
17 |
0 |
20 |
0 |
18 |
0 |
20 |
D5 |
1 |
17 |
0 |
20 |
1 |
18 |
0 |
20 |
Table 7: Number of dead eggs per day and cumulative hatch per replicate in the LS9 group
|
LS/9 |
|||||||
Date |
R1 |
R2 |
R3 |
R4 |
||||
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
|
D1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
D2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
D3 |
0 |
3 |
0 |
3 |
0 |
7 |
0 |
4 |
D4 |
0 |
20 |
0 |
18 |
0 |
19 |
0 |
18 |
D5 |
0 |
20 |
1 |
19 |
0 |
20 |
2 |
18 |
Table 8: Number of dead eggs per day and cumulative hatch per replicate in the LS/3 group
|
LS/3 |
|||||||
Date |
R1 |
R2 |
R3 |
R4 |
||||
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
|
D1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
D2 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
D3 |
0 |
5 |
0 |
4 |
0 |
2 |
0 |
3 |
D4 |
0 |
20 |
0 |
20 |
0 |
20 |
0 |
20 |
D5 |
0 |
20 |
0 |
20 |
0 |
20 |
0 |
20 |
Table 9: Number of dead eggs per day and cumulative hatch per replicate in the LS group
|
LS |
|||||||
Date |
R1 |
R2 |
R3 |
R4 |
||||
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
Dead |
Hatched |
|
D1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
D2 |
14 |
0 |
11 |
0 |
19 |
0 |
19 |
0 |
D3 |
3 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
D4 |
0 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
D5 |
2 |
1 |
6 |
0 |
1 |
0 |
1 |
0 |
Table 10: Number of dead larvae per day in the control group
|
Control group |
|||
Date
|
R01 |
R02 |
R03 |
R04 |
Dead |
Dead |
Dead |
Dead |
|
D4 |
0 |
0 |
0 |
0 |
D5 |
0 |
0 |
0 |
1 |
D6 |
0 |
0 |
0 |
0 |
D7 |
0 |
0 |
0 |
0 |
D8 |
0 |
0 |
0 |
0 |
D9 |
0 |
0 |
0 |
0 |
D10 |
0 |
0 |
0 |
0 |
D11 |
0 |
0 |
0 |
0 |
D12 |
0 |
0 |
0 |
0 |
D13 |
0 |
0 |
0 |
0 |
D14 |
3 |
2 |
1 |
3 |
D15 |
0 |
0 |
0 |
0 |
D16 |
0 |
0 |
0 |
0 |
D17 |
0 |
0 |
0 |
0 |
D18 |
0 |
3 |
2 |
0 |
D19 |
0 |
0 |
0 |
0 |
D20 |
0 |
0 |
0 |
0 |
D21 |
0 |
0 |
0 |
0 |
D22 |
0 |
0 |
0 |
0 |
D23 |
1 |
0 |
1 |
1 |
D24 |
0 |
0 |
0 |
0 |
D25 |
0 |
0 |
0 |
0 |
D26 |
0 |
0 |
0 |
0 |
D27 |
0 |
0 |
0 |
0 |
D28 |
0 |
0 |
0 |
0 |
D29 |
0 |
0 |
0 |
0 |
D30 |
0 |
0 |
0 |
0 |
D31 |
0 |
0 |
0 |
0 |
D32 |
0 |
0 |
0 |
0 |
D33 |
0 |
0 |
0 |
0 |
D34 |
0 |
0 |
0 |
0 |
Table 11: Number of dead larvae per day in the LS/81 group
|
LS/81 |
|||
Date
|
R01 |
R02 |
R03 |
R04 |
Dead |
Dead |
Dead |
Dead |
|
D4 |
0 |
0 |
0 |
0 |
D5 |
0 |
0 |
0 |
0 |
D6 |
0 |
1 |
0 |
0 |
D7 |
0 |
0 |
0 |
0 |
D8 |
0 |
0 |
0 |
0 |
D9 |
0 |
0 |
0 |
0 |
D10 |
0 |
0 |
0 |
0 |
D11 |
0 |
0 |
0 |
0 |
D12 |
0 |
0 |
0 |
0 |
D13 |
0 |
0 |
0 |
0 |
D14 |
2 |
2 |
3 |
4 |
D15 |
0 |
0 |
0 |
0 |
D16 |
0 |
0 |
0 |
0 |
D17 |
0 |
0 |
0 |
0 |
D18 |
2 |
0 |
1 |
0 |
D19 |
0 |
0 |
0 |
0 |
D20 |
0 |
0 |
0 |
0 |
D21 |
0 |
0 |
0 |
0 |
D22 |
0 |
0 |
0 |
0 |
D23 |
1 |
1 |
2 |
0 |
D24 |
0 |
0 |
0 |
0 |
D25 |
0 |
0 |
0 |
0 |
D26 |
0 |
0 |
0 |
0 |
D27 |
0 |
0 |
0 |
0 |
D28 |
0 |
0 |
0 |
0 |
D29 |
0 |
0 |
0 |
0 |
D30 |
0 |
0 |
0 |
0 |
D31 |
0 |
0 |
0 |
0 |
D32 |
0 |
0 |
0 |
0 |
D33 |
0 |
0 |
0 |
0 |
D34 |
0 |
0 |
0 |
0 |
Table 12: Number of dead larvae per day in the LS/27 group
|
LS/27 |
|||
Date
|
R01 |
R02 |
R03 |
R04 |
Dead |
Dead |
Dead |
Dead |
|
D4 |
0 |
0 |
0 |
0 |
D5 |
0 |
0 |
0 |
0 |
D6 |
0 |
0 |
2 |
1 |
D7 |
0 |
0 |
0 |
0 |
D8 |
0 |
0 |
0 |
0 |
D9 |
0 |
0 |
0 |
0 |
D10 |
0 |
0 |
0 |
0 |
D11 |
0 |
0 |
0 |
1 |
D12 |
0 |
0 |
0 |
0 |
D13 |
5 |
6 |
3 |
9 |
D14 |
5 |
7 |
2 |
1 |
D15 |
1 |
0 |
0 |
0 |
D16 |
0 |
0 |
0 |
0 |
D17 |
0 |
0 |
0 |
0 |
D18 |
0 |
0 |
3 |
3 |
D19 |
0 |
1 |
2 |
0 |
D20 |
0 |
0 |
0 |
0 |
D21 |
0 |
0 |
0 |
0 |
D22 |
0 |
0 |
0 |
0 |
D23 |
2 |
3 |
1 |
1 |
D24 |
0 |
0 |
0 |
0 |
D25 |
0 |
0 |
0 |
0 |
D26 |
0 |
0 |
0 |
0 |
D27 |
0 |
0 |
0 |
0 |
D28 |
0 |
0 |
0 |
0 |
D29 |
0 |
0 |
0 |
0 |
D30 |
0 |
0 |
0 |
0 |
D31 |
0 |
0 |
0 |
0 |
D32 |
0 |
0 |
0 |
0 |
D33 |
0 |
0 |
0 |
0 |
D34 |
0 |
0 |
0 |
0 |
Table 13: Number of dead larvae per day in the LS/9 group
|
LS/9 |
|||
Date
|
R01 |
R02 |
R03 |
R04 |
Dead |
Dead |
Dead |
Dead |
|
D4 |
0 |
0 |
0 |
0 |
D5 |
0 |
0 |
0 |
0 |
D6 |
0 |
1 |
0 |
0 |
D7 |
0 |
0 |
0 |
0 |
D8 |
0 |
0 |
0 |
0 |
D9 |
0 |
0 |
0 |
0 |
D10 |
0 |
0 |
0 |
0 |
D11 |
3 |
0 |
0 |
3 |
D12 |
4 |
6 |
4 |
6 |
D13 |
8 |
8 |
1 |
5 |
D14 |
2 |
1 |
11 |
0 |
D15 |
0 |
0 |
0 |
0 |
D16 |
0 |
0 |
0 |
0 |
D17 |
0 |
0 |
0 |
0 |
D18 |
1 |
0 |
0 |
2 |
D19 |
0 |
1 |
1 |
0 |
D20 |
0 |
0 |
0 |
0 |
D21 |
0 |
0 |
0 |
0 |
D22 |
0 |
0 |
0 |
0 |
D23 |
0 |
2 |
2 |
1 |
D24 |
0 |
1 |
0 |
|
D25 |
0 |
0 |
||
D26 |
0 |
0 |
||
D27 |
0 |
0 |
||
D28 |
0 |
0 |
||
D29 |
0 |
0 |
||
D30 |
0 |
0 |
||
D31 |
0 |
0 |
||
D32 |
0 |
0 |
||
D33 |
0 |
0 |
||
D34 |
0 |
0 |
Table 14: Number of dead larvae per day in the LS/3 group
|
LS/3 |
|||
Date
|
R01 |
R02 |
R03 |
R04 |
Dead |
Dead |
Dead |
Dead |
|
D4 |
0 |
0 |
0 |
1 |
D5 |
0 |
1 |
0 |
0 |
D6 |
0 |
2 |
0 |
0 |
D7 |
0 |
0 |
0 |
0 |
D8 |
6 |
0 |
0 |
0 |
D9 |
0 |
0 |
0 |
0 |
D10 |
3 |
0 |
0 |
1 |
D11 |
7 |
3 |
1 |
1 |
D12 |
3 |
7 |
6 |
11 |
D13 |
2 |
3 |
5 |
6 |
D14 |
3 |
5 |
|
|
D15 |
1 |
1 |
|
|
D16 |
0 |
0 |
|
|
D17 |
1 |
1 |
|
|
D18 |
|
1 |
|
Table 15: Number of dead larvae per day in the LS group
|
LS |
|||
Date
|
R01 |
R02 |
R03 |
R04 |
Dead |
Dead |
Dead |
Dead |
|
D3 |
1 |
|
|
|
Description of key information
Long-term toxicity of the registered substance to fish was evaluated in a GLP study according to OECD 210 TG. Embryo and larvae were exposed to graded concentrations of the test item for 34 days in quadruplicate of 20 eggs each under semi-static conditions (control ; limit of solubility LS ; LS/3 ; LS/9 ; LS/27 ; LS/81). Solutions were renewed 3 times a week. Actual concentrations were monitored on a regular basis. Only one larvae hatched at LS and died the following day. All larvae died in the LS/27 treatment. NOEC/EC10 were determined for hatching rate, survival rate and growth (dry weight). The lowest of the three was EC10 for survival rate, which was 5.75 µg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.75 µg/L
Additional information
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