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EC number: 200-198-0 | CAS number: 54-21-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media and from this stock, further test solution was prepared for achieving test concentrations of 100 mg/L.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 145.5 mg of CaCO3
- Test temperature:
- 20±2 °C
- pH:
- 7.3
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
- Conclusions:
- Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia was determine to be > 100 mg/L.
- Executive summary:
Daphnia sp., Acute Immobilization experiment was performed according to OECD Guideline 202, for this the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media (prepared in MilliQ water). A 25 mL of glass beaker was used to perform the experiment which was filled with 20 mL of media including the test chemical.
The test chemical was exposed to daphnia at a limit concentration of 100 mg/L for a period of 48 hours in a static condition.
The median effect Concentration (EC50) of test chemical on Daphnia magna was determined as >100 mg/L on the basis of mobility of the Daphnia magna. Based on the EC50 value it can be consider that the test chemical is nontoxic and can be classified under the category of “not classified chemical” as per the CLP classification criteria.
Reference
Immobility
Nominal Concentration (mg/l) |
Immobility |
|||
Number of Daphnids |
0 h |
24h |
48h |
|
Control |
10 |
- |
- |
- |
100 |
10 |
- |
- |
- |
Any Abnormal Behaviour (if any) |
|
|
|
|
Description of key information
Short term toxicty test was carried out according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) with Daphnia magna as a test organisms.
The effective concentration (EC10) value of test chemical in Daphnia magna in a 24 hr study on mortality effect was determined to be 304 mg/L. Thus,considering the CLP Criteria for aquatic classification of the substance , it is concluded that test substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia magna).
Key value for chemical safety assessment
Additional information
Following experimental studies for the test chemical was summarized to conclude the toxicity of test chemical towards aquatic invertebrate is as follows:
The first experimental study was reviewed from Sewage works journal (1946) in this study the Short term toxicity test was carried out according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) with Daphnia magna as test organisms. The effective concentration (EC10) value of test chemical in Daphnia magna in a 24 hr study on mortality effect was determined to be 304 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance, it is concluded that test substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia magna).
The next study Daphnia sp., Acute Immobilization experiment was performed according to OECD Guideline 202, for this the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media (prepared in MilliQ water). A 25 mL of glass beaker was used to perform the experiment which was filled with 20mL of media including the test chemical. The test chemical was exposed to daphnia at a limit concentration of 100 mg/L for a period of 48 hours in a static condition. The median effect Concentration (EC50) of test chemical on Daphnia magna was determined as >100 mg/L on the basis of mobility of the Daphnia magna. Based on the EC50 value it can be consider that the test chemical is nontoxic and can be classified under the category of “not classified chemical” as per the CLP classification criteria.
To support the above studies another study was reviewed from Ecotoxicology and Environmental Safety journal in this study the short term toxicity test was carried out for 48 hr using daphnia magna as a test organism. Lethal Threshold Concentration value of test chemical in Daphnia magna in a 48 hr study on intoxication effect was determined to be 1450 mg/L. Thus, considering the CLP Criteria for aquatic classification of the substance, it is concluded that test substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia magna)
Thus based on the overall studies the test chemical was concluded to be nontoxic and not classified as per the CLP classification criteria.
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