Dimethylammonium chloride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Experimental determination according to national guideline, no information about GLP

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1980

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EPA, National Eutrophication Research Program, Corvallis, Oreg. (1971)

Principles of method if other than guideline:

Algal assay with Selenastrum capricornutum, in order to determine the 96h EC50 (effective concentration inhibiting the growth for 50 % of the population at 96h).

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Dimethylamine

EC Number:

204-697-4

EC Name:

Dimethylamine

Cas Number:

124-40-3

IUPAC Name:

N-methylmethanamine

Details on test material:

dimethylamine was used as 40 % water solution.

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

320 mg CaCO3/L

Test temperature:

15 °C

pH:

7.4

Dissolved oxygen:

more than 95 % saturation

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

EC0

Effect conc.:

2 mg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

9 mg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

96 h

Dose descriptor:

EC100

Effect conc.:

40 mg/L

Nominal / measured:

not specified

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Reported results for Dimethylamine are EC0 (96h) = 2 mg/L, EC50 (96h) = 9 mg/L, and EC100 (96h) = 40 mg/L, respectively.
Hence, the substance Dimethylamine hydrochloride (CAS-No. 506-59-2, EC-No. 208-046-5), which is a near analogue to Dimethylamine (CAS-No. 124-40-3, EC-No. 204-697-4) should also have an EC0 (96h) of 2 mg/L, an EC50 (96h) of 9 mg/L, and an EC100 (96h) of 40 mg/L, respectively.

Executive summary:

Calamari D. et.al. performed an experiment according to EPA, National Eutrophication Research Program, Corvallis, Oreg. (1971)

to test the acute toxicity of dimethylamine (and other amines) to Selenastrum capricornutum (new name: Pseudokirchnerella subcapitata) in 1980. This test establishes the 96h EC50 (effective concentration inhibiting the growth for 50 % of the population at 96h). Reported results for Dimethylamine are EC0 (96h) = 2 mg/L, EC50 (96h) = 9 mg/L, and EC100 (96h) = 40 mg/L, respectively. Hence, the substance Dimethylamine hydrochloride (CAS-No. 506-59-2, EC-No. 208-046-5), which is a near analogue to Dimethylamine (CAS-No. 124-40-3, EC-No. 204-697-4) should also have an EC0 (96h) of 2 mg/L, an EC50 (96h) of 9 mg/L, and an EC100 (96h) of 40 mg/L, respectively.