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EC number: 212-472-7 | CAS number: 821-06-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP guideline study with concentration control analysis. The analytically determined test concentrations at the end of the experiment were generally below the limit of detection. It is therefore expected that the effect concentrations relate to the hydrolysis product(s) of the test substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (E)-1,4-dibromobut-2-ene
- EC Number:
- 212-472-7
- EC Name:
- (E)-1,4-dibromobut-2-ene
- Cas Number:
- 821-06-7
- Molecular formula:
- C4 H6 Br2
- IUPAC Name:
- 1,4-dibromobut-2-ene
- Details on test material:
- - Name of test substance (as cited in study report): SPP100 PISA P2a
- Description: beige to light brown crystals
- Purity (%): 98.9
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- SAMPLING FOR ANALYSIS
- Samples for possible analysis were taken from all test concentrations and the control according to the schedule below. In addition, the filter used for preparation of the test concentrations was retained for possible analysis of the residue. The method of analysis is described in the appended Analytical Report.
- Frequency: at t=0 h, t=24 h and t=72 h
- Volume: 6 ml
- Storage: Samples were stored in a freezer until analysis.
- At the end of the exposure period, the replicates with algae were pooled at each concentration before sampling.
Test solutions
- Details on test solutions:
- PREPARATION OF TEST SOLUTIONS
- Preparation of test solutions started with a loading rate of 100 mg/l. After a 48-hour stirring period the obtained aqueous mixture was filtered through a 0.45 μm membrane filter (rc 55, Whatman). pH of the filtrate was adjusted from 3.4/3.5 to 6.5 using 1N NaOH (Merck, Darmstadt, Germany). The lower test concentrations were prepared by subsequent dilutions of the filtrate in test medium. The final test solutions were all clear and colourless.
- After preparation, volumes of 50 ml were added to each replicate of the respective test concentration. Subsequently, 1 ml of an algal suspension was added to each replicate providing a cell density of 10^4 cells/ml.
- The test substance was not completely soluble in test medium at the loading rates prepared.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Strain: NIVA CHL 1
- Origin: in-house laboratory culture
STOCK CULTURE
- Algae stock cultures were started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 21 - 24°C
- Light intensity: 60 to 120 µE/m2/s
- Stock culture medium: M1 according to the Nederlandse Praktijk Richtlijn no. 6505
PRE-CULTURE
- 3 days before the start of the test, cells from the algal stock culture were inoculated in culture medium at a cell density of 1 x 10^4 cells/ml. Pre-culture was maintained under the same conditions as used in the test. Cell density was measured immediately before use
- Pre-culture medium: M2 according to OECD 201
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72
Test conditions
- Test temperature:
- 21.8 - 23.7 °C
- pH:
- initial pH: 6.5 - 6.8
final pH: 4.7 - 6.4 - Nominal and measured concentrations:
- Nominal test concentrations: 1.0, 3.2, 10, 32 and 100% of a filtered solution prepared at a loading rate of 100 mg/l
- Details on test conditions:
- Replicates: 3 replicates of each test concentration
6 replicates of the control
1 extra replicate of each test concentration and the control for sampling purposes
2 replicates of the highest test concentration without algae
- Test vessels: 100 ml, all-glass, containing 50 ml of test solution
- Test medium: M2
- Cell density: initial cell density of 1 x 10^4 cells/ml
- Illumination: Continuously using TLD-lamps of the type 'cool-white' of 30 Watt, with a light intensity within the range of 86 to 92 µE.m-2.s-1
- Incubation: Vessels were distributed at random in the incubator. During incubation the algal cells were kept in suspension by continuous shaking - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- EC50 (72 h) 1.2 mg/L based on growth rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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