Registration Dossier
Registration Dossier
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EC number: 202-090-9 | CAS number: 91-68-9
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: scientifically acceptable and valid study, limited documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 957
- Report date:
- 1957
Materials and methods
- Principles of method if other than guideline:
- other: BASF-Test
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-diethylaminophenol
- EC Number:
- 202-090-9
- EC Name:
- 3-diethylaminophenol
- Cas Number:
- 91-68-9
- Molecular formula:
- C10H15NO
- IUPAC Name:
- 3-(diethylamino)phenol
- Details on test material:
- - Name of test material (as cited in study report): Diäthyl-m-aminophenol
- Physical state: solid
- Analytical purity: 99 %
- Other: soluble in acetic acid, alcohol, lutrol, ethylenechloride, unsoluble in soda solution and vegetable oil
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Vienna White
Test system
- Vehicle:
- water
- Controls:
- other: untreated eye of same animal
- Amount / concentration applied:
- - approx. 200 mg of the test substance were applied
- concentration: 50 % in water - Duration of treatment / exposure:
- - the eyes were not washed out after 24 hours
- Observation period (in vivo):
- - 2 days
- Number of animals or in vitro replicates:
- 2
- Details on study design:
- Before OECD Guideline 405 was established, eye irritation was tested using an internal method (BASF test).
White Vienna rabbits were used. 200 mg of the test substance were applied to the conjunctival sac of one eye of 2 animals. The saline-treated adjacent eye served as a control.
The animals were observed several times on the treatment day.
The eyes were not washed out after 24 hours as specified in OECD Guideline 405 and the grading of eye effects was done according a BASF scoring system, that has been converted into the presently used numerical grading system.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- other: 10 min
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- other: 10 min
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- other: 1 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- other: 1 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- other: 3 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- other: 3 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- other: 3 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 10 min
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 1 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- other: 1 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 3 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- other: 3 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h
Applicant's summary and conclusion
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