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Diss Factsheets
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EC number: 229-352-5 | CAS number: 6485-40-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from Committee for Risk Assessment RAC -ECHA
- Qualifier:
- according to guideline
- Guideline:
- other: ISO standard proposal: “water quality – Duckweed growth inhibition test” (2000) and a draft OECD guideline:
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- not specified
- Details on test solutions:
- Test solutions were renewed every 2 days.
- Test organisms (species):
- Lemna minor
- Test type:
- semi-static
- Water media type:
- other: SIS medium
- Limit test:
- no
- Total exposure duration:
- 8 d
- Nominal and measured concentrations:
- 4.6, 10, 22, 46 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of colonies per vessel: 4 plants
- No. of fronds per colony: 10
- No. of vessels per concentration (replicates): 3 (highest concentration, which had six replicates)
- No. of vessels per control (replicates): 6
TEST MEDIUM: SIS test medium was used
OTHER TEST CONDITIONS
- Photoperiod: continuous lighting
- Light intensity and quality: 85-96 µE m-2 s-1.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol.
- Key result
- Duration:
- 4 d
- Dose descriptor:
- EC50
- Effect conc.:
- 52 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0-4 days
- Duration:
- 6 d
- Dose descriptor:
- EC50
- Effect conc.:
- 65 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0-6 days
- Duration:
- 8 d
- Dose descriptor:
- EC50
- Effect conc.:
- 75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0-8 days
- Duration:
- 8 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: other details not available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The EC 50 value was determined to be 52 mg/L, 65 mg/L and 75 mg/L after exposure of test chemical for 0-4, 0- 6- and 0-8 days respectively on aquatic plant Lemna minor. The NOEC value was determined to be 10 mg/L.
- Executive summary:
An experiment was conducted to determine the toxicity of test chemical to aquatic plants according to the ISO standard proposal: “water quality – Duckweed growth inhibition test” (2000) and a draft OECD guideline. The aquatic plant used in this experiment was Lemna minor. The test solutions were renewed every 2 days. The nominal concentration used in study were 4.6, 10, 22, 46 and 100 mg/L along with the control the reference substance (positive control) used was 3,5-dichlorophenol. SIS medium is used as test medium. Three replicates were used for each concentration except the highest concentration and control, which had six replicates. Each replicate (vessel) contains four plants with 10 fronds. The experiment was conducted by using continuous light with intensity of 85-96 µE m-2 s-1. The concentration of test chemical was measured at initiation of experiment after 2 and 6 days of exposure. Frond numbers were counted at the start, after 4, 6 days and at the end of the test of 8 days, simultaneously numbers of affected fronds were recorded. Average growth rate at each test substance concentration was compared with the control value and the percentage reduction in growth was calculated. The mean analytical recovery was 98.1%. Mean actual recoveries were between 91 and 98% of nominal in samples in the period 0-2 days. At day 6 concentrations were above 84%, except at the lowest level (72%). The EC 50 value was determined to be 52 mg/L, 65 mg/L and 75 mg/L after exposure of test chemical for 0-4, 0- 6- and 0-8 days respectively. The NOEC value was determined to be 10 mg/L.
Reference
Average growth rate at each test substance concentration was compared with the control value and the percentage reduction in growth was calculated
Description of key information
The EC 50 value was determined to be 52 mg/L, 65 mg/L and 75 mg/L after exposure of test chemical for 0-4, 0- 6- and 0-8 days respectively on aquatic plant Lemna minor. The NOEC value was determined to be 10 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater plants:
- 75 mg/L
- EC10 or NOEC for freshwater plants:
- 10 mg/L
Additional information
An experiment was conducted to determine the toxicity of test chemical to aquatic plants according to the ISO standard proposal: “water quality – Duckweed growth inhibition test” (2000) and a draft OECD guideline. The aquatic plant used in this experiment was Lemna minor. The test solutions were renewed every 2 days. The nominal concentration used in study were 4.6, 10, 22, 46 and 100 mg/L along with the control the reference substance (positive control) used was 3,5-dichlorophenol. SIS medium is used as test medium. Three replicates were used for each concentration except the highest concentration and control, which had six replicates. Each replicate (vessel) contains four plants with 10 fronds. The experiment was conducted by using continuous light with intensity of 85-96 µE m-2 s-1. The concentration of test chemical was measured at initiation of experiment after 2 and 6 days of exposure. Frond numbers were counted at the start, after 4, 6 days and at the end of the test of 8 days, simultaneously numbers of affected fronds were recorded. Average growth rate at each test substance concentration was compared with the control value and the percentage reduction in growth was calculated. The mean analytical recovery was 98.1%. Mean actual recoveries were between 91 and 98% of nominal in samples in the period 0-2 days. At day 6 concentrations were above 84%, except at the lowest level (72%). The EC 50 value was determined to be 52 mg/L, 65 mg/L and 75 mg/L after exposure of test chemical for 0-4, 0- 6- and 0-8 days respectively. The NOEC value was determined to be 10 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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