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EC number: 255-982-5 | CAS number: 42872-30-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting: 16 March, 2009 - experimental completion: 19 March, 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to international guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-(m-benzoylphenyl)propionitrile
- EC Number:
- 255-982-5
- EC Name:
- 2-(m-benzoylphenyl)propionitrile
- Cas Number:
- 42872-30-0
- Molecular formula:
- C16H13NO
- IUPAC Name:
- 2-(3-benzoylphenyl)propanenitrile
- Details on test material:
- TEST ITEM: Ketoprofen I BPPN
Batch Number: F-CHN-080080-01
Chemical name: 2-(3-Benzoylphenyl)propionitrile
Content: 98.94%
Appearance: Off white powder
Expiry date: 2009-08-12
Recommended storage: Refrigerator, protected from moisture and light
Storage at test facility: 6 ± 2 °C, protected from moisture and light
Retention of test item: At least 1 g has been retained.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: dilution levels 1:1 to 1:8 were diluted with mobile phase
- Sampling method: According to ICH Harmonised Tripartite Guideline (1996) and SANCO 3029/99 rev.4 (2000).
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until start of analysis if necessary.
- Evaluation: Quantification of the active ingredient was calculated by peak area based on the external standard.
Test solutions
- Details on test solutions:
- Stock solution: The stock solution (100 mg/L test item was weighed out) was prepared with dilution water one day prior to application.
Test dilutions of the saturated solution: 1:1 -1:2- 1:4-1:8-1:16 (factor 2) (Equivalent to geometric mean measured test item concentrations: 1.49- 2.90- 6.01 - 12.2- 24.7 mg/L).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUS (Clone 5)
- Source: lnstitut für Wasser-, Boden- und Lufthygiene (WaBolu)
- Age at study initiation: 2 to 24 h old daphnids were used for the definitive study. They were obtained by removing the mother animals twice within 22 h.
- Feeding during test: The daphnids were not fed during the study.
- Food type: a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata,Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of> 10^6 cells/ml.
ACCLIMATION
- Acclimation period: at least 2 h in dilution water
- Acclimation conditions: Diffuse light, illumination strength max. 20 µE.m-2 . s-1, photoperiod: 16/8h ligh/dark cycle
- Type and amount of food: a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata,Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of> 10^6 cells/ml.
- Feeding frequency: At least 5 times per week ad libitum
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Total hardness: 271 mg CaCO3/L
- Test temperature:
- 18 - 22 °C, ± 1 °C constant
- pH:
- At test start: From 7.69 to 7.72
- Dissolved oxygen:
- At test start (0h, new media)
From 6.67 to 8.21 mg/l
Control: 8.32 mg/l - Salinity:
- Conductivity: 656 µS/cm
- Nominal and measured concentrations:
- Geometric Mean Measured Concentration [mg/L]: 1.49, 2.90, 6.01, 12.2, 24.7 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 cm ID x 7 cm H), 50 ml capacity
- Fill volume: 20 mL
- No. of organisms per vessel: 20 animals, divided into 4 parallel samples, each with 5 animals per dilution level and control
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to Directive 92/69/EEC Method C.2, Annex 1
- Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaC03/L.
OTHER TEST CONDITIONS
- Photoperiod: 16h illumination
- Light intensity: illumination strength max. 20 µE.m-2.S-1 .
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.38 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.28 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 6.13 - 6.43
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.17 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 2.90 - 6.01
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 12.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 6.01 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 6.01 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- EC50= 1.95 mg/L (Cl 1.80- 2.12 mg/L). The EC50-value of the reference item potassium dichromate after 24 h is within the prescribed concentration range of 1.0 - 2.5 mg/L of quality criteria according to AQS P 9/2 (05/1996) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.
Any other information on results incl. tables
No data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study Ketoprofen / BPPN was found to be toxic to Daphnia magna after 48 h at concentrations of 6.01 mg/l (LOEC48 h) and higher.
The EC50 (48 h) was determined to be 4.17 mg/l. The NOEC after 48 hours was 2.90 mg/l.
All effect values are given based on the geometric mean measured concentrations of the test item Ketoprofen / BPPN.
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