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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
publication
Title:
OECD eye irritation tests on three alcohols, in: Acute Toxicity Data.
Author:
Jacobs, G.A.
Year:
1990
Bibliographic source:
J Am Coll Toxicol 1: 56-57

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Methanol
EC Number:
200-659-6
EC Name:
Methanol
Cas Number:
67-56-1
Molecular formula:
CH4O
IUPAC Name:
Methyl alcohol

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: untreated eyes of the same animals served as controls
Amount / concentration applied:
TEST MATERIAL
- Amount applied: 0.1 mL
- Concentration: Undiluted
Duration of treatment / exposure:
unspecified (not rinsed, guideline study)
Observation period (in vivo):
1, 4, 24, 48, 72 hours, 8 and 14 days
Number of animals or in vitro replicates:
6
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing: Not rinsed (guideline study)

SCORING SYSTEM: OECD Draize

TOOL USED TO ASSESS SCORE: No data

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Basis:
animal #2
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
2.06
Max. score:
3
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
positive indication of irritation
Irritation parameter:
conjunctivae score
Basis:
animal #1
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
2.06
Max. score:
3
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
positive indication of irritation
Irritation parameter:
chemosis score
Basis:
animal #2
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
0.72
Max. score:
4
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
chemosis score
Basis:
animal #1
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
0.72
Max. score:
4
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
iris score
Basis:
animal #2
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
0.61
Max. score:
2
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
iris score
Basis:
animal #1
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
0.61
Max. score:
2
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
cornea opacity score
Basis:
animal #2
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
0.56
Max. score:
4
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
cornea opacity score
Basis:
animal #1
Remarks:
No individual animal scores available.
Time point:
24/48/72 h
Score:
0.56
Max. score:
4
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
conjunctivae score
Basis:
animal: 1 - 6
Time point:
24/48/72 h
Score:
2.06
Max. score:
3
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
positive indication of irritation
Irritation parameter:
chemosis score
Basis:
animal: 1 - 6
Time point:
24/48/72 h
Score:
0.72
Max. score:
4
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
iris score
Basis:
animal: 1 - 6
Time point:
24/48/72 h
Score:
0.61
Max. score:
2
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Irritation parameter:
cornea opacity score
Basis:
animal: 1 - 6
Time point:
24/48/72 h
Score:
0.56
Max. score:
4
Reversibility:
not fully reversible within: 72 hours
Remarks on result:
probability of weak irritation
Other effects:
not reported

Any other information on results incl. tables

Irritation scores as reported:

 

 

Mean Scores

 

Time after application [h]

 

1

4

24

48

72

Conjunctivitis

0.89

2.00

1.67

2.28

2.22

Chemosis

2.00

2.00

0.67

1.00

0.50

Iritis

0.33

1.00

1.00

1.00

0.33

Corneal opacity

0.00

0.00

0.50

0.50

0.67

 

Applicant's summary and conclusion

Interpretation of results:
not irritating
Conclusions:
The mean scores of the single symptoms did not exceed the respective limits for classification: therefore, this result can be interpreted in terms of a mild irritation potential not requiring classification as eye irritating.